In vitro cultivation of early schistosomula of nasal and visceral bird schistosomes (Trichobilharzia spp., Schistosomatidae)

Chanová, Marta; Bulantová, Jana; Máslo, Petr; Horák, Petr

doi:10.1007/s00436-009-1343-y

Cited by 12 publications

(15 citation statements)

References 35 publications

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“…The surfaces of 3 and 5 days old schistosomula developed in duck, mouse or in vitro differ morphologically from the surface of 1 day old worms (spiniferous tegument of newly transformed schistosomula changes to a surface with pits, holes, and canals; [15]). Nevertheless, the antibody binding pattern of all schistosomula remained similar (Figure 2b-d).…”

Section: Resultsmentioning

confidence: 99%

“…In cercariae, the most intensive specific IgG reaction occurred with glycocalyx ( Figure 2a); cercarial glycocalyx was proven to bind the antibody to a larger extent than any structure of the other stages. The remnants of cercarial glycocalyx still present on 1-day old schistosomula transformed in vitro were labeled with specific antibody, too (for description of T. regenti development in vitro see [15]). This agrees with the results of Kouřilová et al [6].…”

Section: Resultsmentioning

confidence: 99%

“…Released worms were picked up, repeatedly washed with fresh buffer and concentrated by centrifugation. For in vitro cultivation, concentrated cercariae were processed as previously published [15] and then placed into SCM 169 cultivation medium supplied with 0.5% Penicillin/Streptomycin/Amphotericin B (100X; BioWhittaker 17-745E). Schistosomula were collected from the medium after desired period post transformation (p.t.)…”

Section: Parasitesmentioning

confidence: 99%

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Trichobilharzia regenti: Antigenic structures of intravertebrate stages

Chanová

Lichtenbergová²,

Bulantová

et al. 2011

Open Life Sciences

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Like several other bird schistosomes, neurotropic schistosome of Trichobilharzia regenti can invade also mammals, including humans. Repeated infections cause cercarial dermatitis, a skin inflammatory reaction leading to parasite elimination in non-specific mammalian hosts. However, in experimentally primo-infected mice, the worms escape from the skin and migrate to the central nervous system. In order to evade host immune reactions, schistosomes undergo cercaria/schistosomulum transformation accompanied with changes of surface antigens. The present study is focused on localization of the main antigens of T. regenti; cercariae, schistosomula developed under different conditions and adults were compared. Antigens were localized by immunofluorescence and ultrastructural immunocytochemistry using sera of mice repeatedly infected with T. regenti. Detected antibody targets were located in glycocalyx and penetration glands of cercariae and in tegument of cercariae, schistosomula and adults. Shedding of cercarial glycocalyx significantly reduced surface reactivity; further decrease was reported during ongoing development of schistosomula. Spherical bodies, probably transported from subtegumental cell bodies to worm surface, were identified as the most reactive tegumental structures. Based on similar results for schistosomula developed in specific, non-specific hosts and in vitro, it seems that the ability of T. regenti to decrease the surface immunoreactivity during ontogenesis is independent on the host type.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Parasitesmentioning

confidence: 99%

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Trichobilharzia regenti: Antigenic structures of intravertebrate stages

Chanová

Lichtenbergová²,

Bulantová

et al. 2011

Open Life Sciences

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“…Disruption of hemocyte signaling pathways, such as protein kinase C (PKC) and extracellular signal-regulated kinase (ERK) pathways, may also influence hemocyte activities (150)(151)(152). For example, carbohydrates known to be present on larval surfaces of T. szidati and T. regenti, such as D-galactose and L-fucose (153)(154)(155)(156), affected hemocyte PKC and ERK signaling in L. stagnalis, which suggests an immunosuppressive role (157,158). However, experiments investigating the direct effect of Trichobilharzia larvae on hemocyte signaling have not been performed.…”

Section: Molluscan and Avian Host Specificitymentioning

confidence: 99%

Avian Schistosomes and Outbreaks of Cercarial Dermatitis

et al. 2015

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SUMMARY Cercarial dermatitis (swimmer's itch) is a condition caused by infective larvae (cercariae) of a species-rich group of mammalian and avian schistosomes. Over the last decade, it has been reported in areas that previously had few or no cases of dermatitis and is thus considered an emerging disease. It is obvious that avian schistosomes are responsible for the majority of reported dermatitis outbreaks around the world, and thus they are the primary focus of this review. Although they infect humans, they do not mature and usually die in the skin. Experimental infections of avian schistosomes in mice show that in previously exposed hosts, there is a strong skin immune reaction that kills the schistosome. However, penetration of larvae into naive mice can result in temporary migration from the skin. This is of particular interest because the worms are able to migrate to different organs, for example, the lungs in the case of visceral schistosomes and the central nervous system in the case of nasal schistosomes. The risk of such migration and accompanying disorders needs to be clarified for humans and animals of interest (e.g., dogs). Herein we compiled the most comprehensive review of the diversity, immunology, and epidemiology of avian schistosomes causing cercarial dermatitis.

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“…Recent in vitro cultivation studies have shown experimental approaches using animal sera for growth and maturation of worms; gymnophallids [11], microphallids [12,13], and schistosomatids [14]. Stewart et al [15] developed in vitro strigeid metacercariae of Apatemon cobitidis proterorhini and Cotylurus erraticus to ovigerous adults in 5 days at 41℃, using a semi-solid culture medium of chicken serum and egg albumen.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Cultivation of Cymatocarpus solearis (Brachycoeliidae) Metacercariae to Obtain the Adult Stage without the Marine Turtle Definitive Host

Grano-Maldonado

Álvarez-Cadena

2010

Korean J Parasitol

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Abstract:In vitro cultivation of trematodes would assist studies on the basic biology of the parasites and their hosts. This is the first study to use the yolk of unfertilized chicken eggs as a simple and successful method of ovocultivation and the first time to obtain the adult-stage of the trematode Cymatocarpus solearis Braun, 1899 (Digenea: Brachycoeliidae). Chicken eggs were inoculated with metacercariae from the muscle of the spiny lobster, Panulirus argus (Latreille, 1804). The metacercariae were excysted and incubated for 576 hr (24 days) at 38°C to obtain the adult stage. Eggs in utero were normal in shape and light brown color. The metacercariae developed into mature parasites that have been identified as the adultstage found in marine turtles. The adult lobsters collected in Quintana Roo State, Mexico, showed the prevalence of 49.4% and the mean intensity of 26.0 per host (n = 87). A statistical study was performed to determine that no parasitic preference was detected for male versus female parasitized lobsters. Morphometric measurements of the adult-stage of C. solearis obtained in our study have been deposited in the National Helminths Collection of the Institute of Biology of the National Autonomous University of Mexico. This study is significant because it is the first time that a digenean of the family Brachycoeliidae has been demonstrated to develop in vitro from metacercariae into adults capable of producing eggs using the yolk of unfertilized chicken eggs. Secondly, this technique allows to obtain the adult stage of C. solearis without the presence of its marine turtle host, allows us to describe the mature parasites, and thus contribute to our understanding of the biology of C. solearis.

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In vitro cultivation of early schistosomula of nasal and visceral bird schistosomes (Trichobilharzia spp., Schistosomatidae)

Cited by 12 publications

References 35 publications

Trichobilharzia regenti: Antigenic structures of intravertebrate stages

Trichobilharzia regenti: Antigenic structures of intravertebrate stages

Avian Schistosomes and Outbreaks of Cercarial Dermatitis

In Vitro Cultivation of Cymatocarpus solearis (Brachycoeliidae) Metacercariae to Obtain the Adult Stage without the Marine Turtle Definitive Host

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