In vitro cultivation of Babesia canis canis parasites isolated from dogs in Poland

Adaszek, Łukasz; Winiarczyk, S.

doi:10.1007/s00436-010-2181-7

Cited by 9 publications

(6 citation statements)

References 21 publications

(24 reference statements)

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“…Moreover, two species, Babesia felis -like and Babesia microti -like (now considered as Theileria annae ), were detected in Germany in dogs imported from Romania, and in Spain, respectively (Hamel et al 2012; Matijatko et al 2012). Among these Babesia species, only B. canis has been detected in Poland (Adaszek and Winiarczyk 2011). …”

Section: Introductionmentioning

confidence: 99%

Increased concentration of serum TNF alpha and its correlations with arterial blood pressure and indices of renal damage in dogs infected with Babesia canis

Zygner

Gójska-Zygner²,

Bąska

et al. 2014

Parasitol Res

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Canine babesiosis is a tick-borne disease caused by parasites of the genus Babesia. Tumour necrosis factor alpha (TNF-α) is a cytokine that plays a role in the pathogenesis of canine babesiosis. In this study, the authors determined the concentration of serum TNF-α in 11 dogs infected with Babesia canis and calculated Spearman’s rank correlations between the concentration of TNF-α and blood pressure, and between TNF-α and indices of renal damage such as: fractional excretion of sodium (FE(Na+)), urinary creatinine to serum creatinine ratio (UCr/SCr), renal failure index (RFI), urine specific gravity (USG) and urinary protein to urinary creatinine ratio (UPC). The results demonstrated statistically significant strong negative correlations between TNF-α and systolic arterial pressure (r = −0.7246), diastolic arterial pressure (r = −0.6642) and mean arterial pressure (r = −0.7151). Serum TNF-α concentration was also statistically significantly correlated with FE(Na+) (r = 0.7056), UCr/SCr (r = −0.8199), USG (r = −0.8075) and duration of the disease (r = 0.6767). The results of this study show there is an increase of serum TNF-α concentration during canine babesiosis, and the increased TNF-α concentration has an influence on the development of hypotension and renal failure in canine babesiosis. This probably results from the fact that TNF-α is involved in the production of nitric oxide and induction of vasodilation and hypotension, which may cause renal ischaemia and hypoxia, and finally acute tubular necrosis and renal failure.

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Section: Introductionmentioning

confidence: 99%

Increased concentration of serum TNF alpha and its correlations with arterial blood pressure and indices of renal damage in dogs infected with Babesia canis

Zygner

Gójska-Zygner²,

Bąska

et al. 2014

Parasitol Res

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“…Species such as Babesia canis, B. vogeli, B. gibsoni, B. felis-like and B. microti-like have been detected in dogs in Europe (Cacci o et al 2002;Hamel et al 2012;Matijatko et al 2012). Among these species only B. canis has been detected in dogs in Poland (Welc-Falęciak et al 2009;Adaszek & Winiarczyk 2011).…”

Section: Introductionmentioning

confidence: 99%

Hyperaldosteronism and its association with hypotension and azotaemia in canine babesiosis

Gójska-Zygner¹,

Zygner

2014

Veterinary Quarterly

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Background: The previous work on canine babesiosis revealed hypokalaemia and increased fractional excretion of potassium in infected dogs suggesting a role for aldosterone in the loss of potassium via the kidneys in affected dogs. Moreover, hypotension, which is one of the complications of canine babesiosis leading to renal failure, may induce secondary hyperaldosteronism. Animals and methods: In this study, the serum aldosterone concentration was determined in 14 dogs infected with Babesia canis. The MannÀWhitney U test was used to compare serum aldosterone concentration and blood pressure between these dogs and 10 healthy dogs. Spearman's rank correlations between serum aldosterone concentration and blood pressure and between serum aldosterone and urea and creatinine concentrations were calculated. Results: Increased concentrations of aldosterone above reference intervals were observed in only 4 out of the 14 dogs. The results showed significantly (p < 0.05) higher serum aldosterone concentrations and lower blood pressures in infected dogs in comparison to healthy dogs, and significantly negative correlations between aldosterone concentration and systolic arterial pressure (r D ¡0.63), diastolic arterial pressure (r D ¡0.67) and mean arterial pressure (r D ¡0.65). Serum aldosterone concentration was also significantly correlated with serum urea concentration (r D 0.72), serum creatinine concentration (r D 0.69) and serum potassium concentration (r D ¡0.57). Conclusion:The results of this study show hyperaldosteronism in some cases of canine babesiosis as a possible response to hypotension. However, both the hypotension and severe azotaemia observed in dogs infected with B. canis and associated hyperaldosteronaemia suggest that this response is insufficient.

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“…The process for determining and breeding Babesia canis strains 18S RNA-A and 18S RNA-B was presented in an earlier study (4). Briefly, supernatant media from in vitro culture were collected and mixed when parasitemias reached the level of 0.6%.…”

Section: Methodsmentioning

confidence: 99%

Preliminary Study on the Safety of a New Vaccine Against Canine Babesiosis Containing Soluble Parasitic Antigen (SPA)

Adaszek

Wernicka-Furmaga

Winiarczyk

2012

Bulletin of the Veterinary Institute in Pulawy

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The aim of this study was to assess the safety of a new vaccine, containing soluble parasitic antigen (SPA), against canine babesiosis. Fifteen dogs were included in the experiment. Five controls received only the adjuvant and 10 dogs were vaccinated with Babesia canis canis SPA twice, at a two weeks interval. For the whole period of the study all animals were under constant clinical observation. Haematological and biochemical tests were performed. Flu-like symptoms and local reactions at the injection site were observed in three animals from the vaccinated group and in two dogs from the control group. These events were transient, receded spontaneously and did not require any appropriate treatment. In 50% of the vaccinated dogs, a slight and spontaneously receding thrombocytopenia developed. However, in none of the animals used in the experiment shock symptoms were observed. Administration of the SPA did not affect the functions of internal organs, which was confirmed by normal results of biochemical tests. The obtained Babesia canis SPA can be considered safe and well tolerated by dogs, and therefore it can be used in further studies on the immunisation of animals against babesiosis.

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In vitro cultivation of Babesia canis canis parasites isolated from dogs in Poland

Cited by 9 publications

References 21 publications

Increased concentration of serum TNF alpha and its correlations with arterial blood pressure and indices of renal damage in dogs infected with Babesia canis

Increased concentration of serum TNF alpha and its correlations with arterial blood pressure and indices of renal damage in dogs infected with Babesia canis

Hyperaldosteronism and its association with hypotension and azotaemia in canine babesiosis

Preliminary Study on the Safety of a New Vaccine Against Canine Babesiosis Containing Soluble Parasitic Antigen (SPA)

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