In Vitro Conversion of Thiocyanate to Cyanide in the Presence of Erythrocytes.

Pines, Kermit L.; Crymble, Margaret M.

doi:10.3181/00379727-81-19810

Cited by 31 publications

(4 citation statements)

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“…Although the authors of the study were not able to unequivocally identify the enzyme responsible for this reaction, they named this enzyme thiocyanate oxidase [199]. Independent studies confirmed that cyanide generation could be detected within 10 min after the exposure of human blood cells (but not plasma) to thiocyanate [268]. In a follow‐up study, systemic fractionation of hemolysate showed that cyanide formation was present in all the fractions containing hemoglobin, and it was directly proportional the concentration of the enzyme.…”

Section: Elimination Of Cyanide In Mammalian Cells and Organismsmentioning

confidence: 99%

The two faces of cyanide: an environmental toxin and a potential novel mammalian gasotransmitter

Zuhra

Szabó

2021

The FEBS Journal

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Section: Elimination Of Cyanide In Mammalian Cells and Organismsmentioning

confidence: 99%

The two faces of cyanide: an environmental toxin and a potential novel mammalian gasotransmitter

Zuhra

Szabó

2021

The FEBS Journal

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“…Cyanide is generated from glycine by the reaction with chlorination reagents, such as chloramine T or hypochlorous acid. 12) Glutathione is a peptide containing glycine. Cyanide generated by this reaction may be detected.…”

Section: Separation Of Cyanide Thiocyanate and Selenocyanatementioning

confidence: 99%

“…There are some reports which show the conversion of thiocyanate to cyanide in the presence of red blood cells. 8,13) It has been suggested that the ferric ion (Fe 3+ ) from methemoglobin mediates the degradation reaction of thiocyanate into cyanide. To examine if selenocyanate could be decomposed into cyanide in the same manner as thiocyanate, selenocyanate solution was added to iron(III) chloride methanolic solution.…”

Section: Separation Of Cyanide Thiocyanate and Selenocyanatementioning

confidence: 99%

Detection of Selenocyanate in Biological Samples by HPLC with Fluorescence Detection Using König Reaction

Mochizuki

Higashi

Okamoto

et al. 2019

CHEMICAL & PHARMACEUTICAL BULLETIN

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We developed a simple and sensitive HPLC method for the determination of selenocyanate (SeCN). The König reaction, which is generally used for the determination of cyanide and thiocyanate, was applied for the post-column detection, and using barbituric acid as a fluorogenic reagent made it possible to detect SeCN with high sensitivity. The limits of detection (LOD) and quantification (LOQ) were 73.5 fmol and 245.1 fmol, respectively. Subsequently, the amounts of SeCN in human blood and in cultured cell samples were analyzed, and no SeCN was detected in human whole blood. Interestingly, we have found that some of the spiked SeCN decomposed to cyanide in human whole blood. Ascorbic acid suppressed the decomposition of SeCN to cyanide by reducing the ferric ion, which is typically involved in SeCN decomposition. Then, SeCN was detected in cultured HEK293 cells exposed to selenite. The established HPLC method with fluorescence detection of SeCN is useful for investigating small amounts of SeCN in biological samples.

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“…Later toxicity, manifested by fatigue, weakness, stupor, psychoneurosis, aphasia, slurred speech, muscle twitching, hyperhidrosis, tinnitus, and hypothyroidism (WALD et aI., 1939;PAGE, 1955;NOUROK et aI., 1964;AHEARN and GRIM, 1974), appears to be related to accumulation of thiocyanate (DOMZALSKI et aI., 1953;PAGE, 1955). Cyanide itself could also contribute to such intoxication after prolonged administration (MOISTER and FREIS, 1949;GOLDSTEIN and RIEDERS, 1951;PINES and CRYMBLE, 1952;VESEY et aI., 1974). Nitroprusside administration should be discontinued when plasma thiocyanate levels exceed 10 to 12 mgjdl (PAGE, 1955;GIFFORD, 1961;AHEARN and GRIM, 1974).…”

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confidence: 99%