In vitro Conservation Technique for Russian Citrus limon

Abstract

In vitro storage conditions of Citrus limon (L.) Burm. cv. Novoafonsky were developed in this study. Three explants were used: nodal regenerants, seedlings, and micrografted plants. The results obtained in present investigation confirmed that slow growth maintenance of lemon microplants on MS medium allows to store micro-grafted plants and seedlings for 8 and 12 months, respectively, without subculturing. It was also noted that low temperature ?10 ± 2°C and light intensity of 1,000 lux maintain seedlings till 12 months and micrografted plants till 8 months on MS medium. The decrease of chlorophyll and carotenoids in leaves was observed during the storage of microplants. It was observed that low temperature and low light intensity were better for the preservation of the photosynthetic apparatus and accumulation of photosynthates in the leaves of lemon regenerants, thereby maintaining a high level of their viability. Thus, the best treatment for in vitro conservation was micrografted plants on MS media in temperature ?10 ± 2°C and light intensity of 1,000 lux.

Minimal Media Strength for In vitro Conservation of Strawberry (Fragaria ananassa) Cultures

“…The results of tables (1 and 2) showed a significant effect of the concentration of medium MS salts on the percentage of cultures viability and regeneration ability (shoots multiplication and rooting), these results agreed with what Ahmed et al (2010), Samarina et al (2014) and Sota & Kongjika (2019) who found that the survival % and shoots multiply decreases with increasing conservation time, but it remained survival.…”

“…One of the storage methods is minimal of nutrient medium by reducing the amount of nutrients. This method has been successfully used in storing different vegetatively propagated horticultural plants, such as, Citrus limon (Samarina et al, 2014), Ziziphus jujube Mill (Sota & Kongjika, 2014), and Prunus webbii (Sota & Kongjika, 2019).…”

Тhe influence of retardants on the reduction of growth rate and the preservation of viability of sterile cultures

“…Deceleration of growth of the culture can be accomplished by lowering of temperature of cultivation (Dale, 1980;Henshaw et al, 1985;Ruredzo& Hanson, 1991;Dorion et al, 1994;HaeBoong et al, 1996;Samarina et al, 2014;Ali et al, 2016), in combination with low illumination or darkness (Mullin and Schlegel, 1976), changes in the concentration of carbonhydrate and mineral composition of the nutrient medium (Ng and Ng, 1991;Samsonova, 1991;Vysotskaya, 1994;Homes et al, 1982;Rubluo and Kartha, 1985;Rolland et al, 2006), a decrease in oxygen in culture vessels (Brindgen and Staby, 1981;Muromtsev et al, 1990;Winthers, 1979;Brindgen and Staby, 1983;Dorion et al, 1994), the induction of osmotic stress (Henshaw et al, 1980;Ng and Ng, 1991;Ruredzo and Hanson, 1991;Tukai et al, 1988;Codaccioni and Vescovi, 1987;Schilde-Renther et al, 1982;Wanas et al, 1986;Pua and Chong, 1985;Rahman et al, 2010;Ciobaniui DOI: http://dx.doi.org/10.22192/ijarbs.2017.04.05.007 andConstantinovici, 2012), the use of retardants (Westcott, 1981;Cathey, 1964;Zadontsev et al, 1973;Chailakhyan, 1967;Kende et al, 1963).…”