In Vitro Conservation of<i>Turbinicarpus</i>(Cactaceae) Under Slow Growth Conditions

Pérez-Molphe-Balch, Eugenio; Pérez-Reyes, Martha Evelia; Rosa-Carrillo, Ma. de Lourdes de la

doi:10.2985/1070-0048-17.1.6

Cited by 17 publications

(3 citation statements)

References 17 publications

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“…Nevertheless, Tacátzcuaro Especial has the highest number of regenerated plants. These results are in accordance with other minimal growth reports in which lower explant regeneration than explant survival at the end of the conservation period was obtained [44,45]. This suggests that plant survival is not sufficient to choose an adequate number of initial plants for medium-term conservation.…”

Section: Discussionsupporting

confidence: 91%

In Vitro Conservation of Mexican Garlic Varieties by Minimal Growth

Tirado,

Gómez-Rodríguez,

Cruz-Cárdenas

et al. 2023

Plants

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Garlic (Allium sativum L.) is one of the 30 crops that are essential for world food; therefore, its conservation should be considered a priority. There are two main plant conservation strategies: in situ and ex situ conservation. Both strategies are important; nevertheless, ex situ field conservation is affected by biotic and abiotic factors. A complementary strategy to preserve garlic germplasm in the medium term is through in vitro culture by minimal growth. The aim of this study was to evaluate the in vitro conservation of three Mexican garlic varieties by minimal growth. Garlic plants obtained from in vitro garlic bulbs were preserved in six culture media at 25, 18, and 5 °C. A randomized design was used and an analysis of the variance of the survival, contamination, and shoot height of the explants was performed at 30, 60, 90, 180, 270, and 365 days of culture. The results showed that the in vitro conservation of Pebeco, Tacátzcuaro Especial, and Huerteño garlic varieties was optimally obtained for one year at 5 °C in a basal Murashige and Skoog (MS) culture medium with 68.46 g L−1 sucrose and 36.43 g L−1 sorbitol. Thus, the achieved protocol can be adapted to other varieties of garlic for medium-term storage in germplasm banks.

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Section: Discussionsupporting

confidence: 91%

In Vitro Conservation of Mexican Garlic Varieties by Minimal Growth

Tirado,

Gómez-Rodríguez,

Cruz-Cárdenas

et al. 2023

Plants

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“…The high %S under stress conditions observed for both species of cacti might be related to the morphophysiological mechanisms that M. paucispinus and M. glaucescens have in order to survive under stress conditions where their populations are exposed in their natural environment, characterized by long drought periods (Pérez-Molphe-Balch et al 2012). Both species have a small genome that: (i) allows rapid cycles of cell division in the root cells when water is available; and (ii) allows up to four rounds of endoreduplication of the cortex cells, which are capable of major expansion to optimize water storage (Torres- Silva et al 2020).…”

Section: Discussionmentioning

confidence: 97%

“…Molecular characterization allows the identi cation of duplicates and reduction in the number of samples to be conserved, which is necessary to reduce the costs of in vitro conservation (Rao and Hodgkin 2002;Nick et al 2010;Martín et al 2013). Genetic diversity analysis also provides information that may positively contribute to the use of germplasm successfully stored in future ecosystem restoration programs, including the reintroduction of species that have ex situ conservation (Rao and Hodgkin 2002;Volis and Blecher 2010;Pérez-Molphe-Balch et al 2012). Among DNA molecular markers, Inter Simple Sequence Repeats (ISSR) have been frequently used for molecular characterization of plants from ex situ conservation (Luna-Paez et al 2007;Oliveira et al 2013), due to the low cost and little infrastructure demanded, high reproducibility and polymorphism, and good e ciency in distinguishing variability between individuals (Ganopoulos et al 2015;Grover and Sharma 2016).…”

Section: Introductionmentioning

confidence: 99%

In vitro conservation and genetic diversity of threatened species of Melocactus (Cactaceae)

et al. 2021

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The high endemism, the natural habitat degradation, and the over-collection for ornamental purposes have led some species such as Melocactus paucispinus and M. glaucescens to be threatened with extinction. The use of in vitro conservation techniques, such as slow growth storage, promotes the preservation of genetic diversity with integrity. The goal of this study was to establish a strategy for in vitro conservation of apical segments of the cladode of M. paucispinus and M. glaucescens and evaluate the genetic diversity of individuals from in vitro germinated plants. For such purpose, different concentrations of the plant regulator ancymidol and the osmotic agent sucrose on the inhibition of the in vitro growth were tested, and the genetic diversity of M. paucispinus and M. glaucescens individuals stored in vitro was evaluated. Sucrose showed higher e ciency in the reduction of growth than ancymidol for both species. However, due to the reduction in survival percentage, the use of sucrose over 75 g L − 1 in the in vitro conservation of both species for 360 days is not recommended. In the genetic diversity analysis, 76.92% of polymorphic loci (P), expected heterozygosity (He) = 0.276 and Shannon index (S) = 0.414 were observed for M. paucispinus. For M. glaucescens, the observed values were P = 95.38%, He = 0.228 and S = 0.369. These values observed here were higher than those previously found for the natural populations of these species, which demonstrated that this in vitro collection showed genetic diversity and can be used in management and reintroduction programs of these species.

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