In vitro conservation of Amburana cearensis (Fabaceae)

Abstract

<jats:p>ABSTRACT: Slow-growth tissue culture allows in vitro conservation of plant genetic resources and is a complementary technique to conventional preservation methods. The objective of this study was to evaluate the effect of the culture medium supplemented with sucrose, mannitol and sorbitol osmotic agents on the induction of in vitro slow growth of A. cearensis, seeking to establish alternative techniques for ex situ conservation of the species. Plants with age of 10 days were inoculated in woody plant medium (WPM) supplemented with different concentrations of sucrose (87.64, 131.46, 175.28 or 262.92 mM) combined with mannitol or sorbitol at varying concentrations (0.0, 43.8, 65.73, 87.64 or 131.46 mM), solidified with 0.7% agar. The following parameters were evaluated: survival percentage, number of senescent leaves, aerial part length, root length, aerial part dry mass, root dry mass; and number of shoots. The conservation of A. cearensis was viable for up to 300 days in WPM supplemented with 262.92 mM of sucrose or the combination of 87.64 mM of sucrose with 43.80 mM of mannitol, because besides achieving a survival percentage greater than 55.0%, the plants conserved in these media presented normal development and the best appearances, with predominance of uniform green leaves during the storage process.</jats:p>

Cinchona officinalis L. in vitro conservation by means of slow-growth storage and cryopreservation techniques

“…Root formation was observed at a higher percentage in the presence of sucrose and lower for sorbitol and mannitol. Matos et al (2020) reported similar results in the Amburana cearensis cultivar. Water stress caused by the presence of mannitol and sorbitol does not stimulate root development (Gopal and Chauhan 2010), as it reduces the turgor pressure necessary for cell division, as observed in Psychotria ipecac (Urrea-Trujillo et al…”

Konservasi In Vitro Kantong Semar (Nepenthes rafflesiana Jack.) dengan Metode Slow Growth

“…Penelitian terkait konservasi in vitro dengan penerapan teknik slow growth telah dilakukan untuk tanaman kelapa sawit (Elaeis guineensis dan E. oleifera) [10], Senecio macrophyllus [11], anggrek endemik di Meksiko Laelia anceps Lindl. [12], Vanilla planifoliaJacks [13], tanaman obat Dioscorea floribunda [14] dan Amburana cearensis [15].…”

Use of micropropagation in the vegetative rescue of adult trees of Cedrela odorata L.

“…The oxidation observed can be attributed to regulator levels or phenolic oxidation. High concentrations of growth regulators can cause intoxication and oxidation of explants, indicative of inadequate concentrations (Almeida et al, 2020), as well as modify or inhibit explant growth (Lima et al, 2020). The browning resulting from phenol oxidation is due to polyphenol oxidase activity, which results in the production of quinones, which are melanic compounds that contribute to browning and death of explants (Huh et al, 2017).…”

“…Using MS culture medium, with activated charcoal and combinations of 2.2 μM of BAP and 2.68 μM of NAA, Huamán et al (2012) obtained, after five weeks of C. odorata culture, 100% rooting, 8.13 roots per explant and 10.43 cm in length. Activated carbon can be used in the composition of the rooting medium, for its capacity to absorb inhibiting and toxic residual substances from the previous phase of micropropagation, promoting better rooting rates (Lima et al, 2020).…”

“…The rescue of forest species by vegetative propagation can be influenced by the ontogenetic age of the tissue, and various techniques are applied to rejuvenate and/or invigorate plants (Stuepp et al, 2018). According to Alvim et al (2020), in forest species with low propagative rate by seeds, micropropagation enables in vitro conservation of invigorated material, mass multiplication and the selection of genetically superior matrices.…”