2020
DOI: 10.1590/0103-8478cr20190729
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In vitro conservation of Amburana cearensis (Fabaceae)

Abstract: ABSTRACT: Slow-growth tissue culture allows in vitro conservation of plant genetic resources and is a complementary technique to conventional preservation methods. The objective of this study was to evaluate the effect of the culture medium supplemented with sucrose, mannitol and sorbitol osmotic agents on the induction of in vitro slow growth of A. cearensis, seeking to establish alternative techniques for ex situ conservation of the species. Plants with age of 10 days were inoculated in woody plant m… Show more

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Cited by 3 publications
(5 citation statements)
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References 19 publications
(24 reference statements)
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“…Root formation was observed at a higher percentage in the presence of sucrose and lower for sorbitol and mannitol. Matos et al (2020) reported similar results in the Amburana cearensis cultivar. Water stress caused by the presence of mannitol and sorbitol does not stimulate root development (Gopal and Chauhan 2010), as it reduces the turgor pressure necessary for cell division, as observed in Psychotria ipecac (Urrea-Trujillo et al…”
Section: Root Formationsupporting
confidence: 63%
“…Root formation was observed at a higher percentage in the presence of sucrose and lower for sorbitol and mannitol. Matos et al (2020) reported similar results in the Amburana cearensis cultivar. Water stress caused by the presence of mannitol and sorbitol does not stimulate root development (Gopal and Chauhan 2010), as it reduces the turgor pressure necessary for cell division, as observed in Psychotria ipecac (Urrea-Trujillo et al…”
Section: Root Formationsupporting
confidence: 63%
“…Penelitian terkait konservasi in vitro dengan penerapan teknik slow growth telah dilakukan untuk tanaman kelapa sawit (Elaeis guineensis dan E. oleifera) [10], Senecio macrophyllus [11], anggrek endemik di Meksiko Laelia anceps Lindl. [12], Vanilla planifoliaJacks [13], tanaman obat Dioscorea floribunda [14] dan Amburana cearensis [15].…”
Section: Pendahuluanunclassified
“…The oxidation observed can be attributed to regulator levels or phenolic oxidation. High concentrations of growth regulators can cause intoxication and oxidation of explants, indicative of inadequate concentrations (Almeida et al, 2020), as well as modify or inhibit explant growth (Lima et al, 2020). The browning resulting from phenol oxidation is due to polyphenol oxidase activity, which results in the production of quinones, which are melanic compounds that contribute to browning and death of explants (Huh et al, 2017).…”
Section: /7mentioning
confidence: 99%
“…Using MS culture medium, with activated charcoal and combinations of 2.2 μM of BAP and 2.68 μM of NAA, Huamán et al (2012) obtained, after five weeks of C. odorata culture, 100% rooting, 8.13 roots per explant and 10.43 cm in length. Activated carbon can be used in the composition of the rooting medium, for its capacity to absorb inhibiting and toxic residual substances from the previous phase of micropropagation, promoting better rooting rates (Lima et al, 2020).…”
Section: Root Induction and Acclimatizationmentioning
confidence: 99%
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