In vitro compatibility of microbial agents for simultaneous application to control strawberry powdery mildew (Podosphaera aphanis)

Sylla, Justine; Alsanius, Beatrix; Krüger, Erika; Becker, Dorit; Wohanka, W.

doi:10.1016/j.cropro.2013.04.011

Cited by 20 publications

(7 citation statements)

References 32 publications

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“…The three BCAs B. amyloliquefaciens, A. pullulans and B. bassiana have already been tested for their compatibility in vitro in a previous study and only inhibitory effects of B. amyloliquefaciens on the mycelial growth of A. pullulans were observed in vitro (Sylla et al 2013a). However, that study was not aimed to elucidate their modes of action.…”

Section: Discussionmentioning

confidence: 97%

Control of Botrytis cinerea in strawberries by biological control agents applied as single or combined treatments

et al. 2015

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There is little knowledge about the effects of simultaneously applied biological control agents (BCAs) on the incidence of grey mould (Botrytis cinerea) in strawberries under field conditions. Our objective was, therefore, to investigate the use of three BCAs (Bacillus amyloliquefaciens FZB42, Aureobasidium pullulans DSM 14940 and DSM 14941 and Beauveria bassiana ATCC 74040) with respect to their efficacy against B. cinerea in field grown strawberries following single compared to combined application. In two subsequent years (2011 and 2012), improved control of B. cinerea resulted from combined BCA treatments, whereas the single BCAs were not efficient. Efficient combinations of BCA treatments were not the same in 2011 and 2012, however. The development of B. cinerea was reduced by single treatments of B. amyloliquefaciens and A. pullulans as well as by most combined BCA treatments during storage for one of two fruit samples in 2011. In 2012, however, no effects on B. cinerea during storage resulted from BCA treatments. The results of the present study suggest that the use of combined BCA treatments only cannot overcome inconsistent efficacies of BCAs against strawberry grey mould under field conditions. Further investigations on the interactions between the BCAs in combined BCA treatments as well as between the BCAs and the pathogen in dependence on multiple factors prevailing in the phyllosphere, however, need to be included to tailor combined BCA treatments with constant efficacies in different years.

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Section: Discussionmentioning

confidence: 97%

Control of Botrytis cinerea in strawberries by biological control agents applied as single or combined treatments

et al. 2015

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“…Bacteria are the most abundant group of plant colonizers, and the number of bacteria differs depending on the plant part (rhizosphere: 10 7 -10 10 colony-forming units [CFU] g 1 root; phyllosphere: 10 6 -10 9 CFU g 1 leaf [Timms- ]; endosphere: 10 3 -10 5 CFU g 1 tissue [Lodewyckx et al 2002]; carposphere: 10 5 CFU g 1 fruit). These microenvironments may be colonized by specific bacterial communities, and shifts occur as physiological age increases (Berg et al 2005;Sylla et al 2013a;Rosberg et al 2014a,b). The microbial community structure varies between different plant species (Garbeva et al 2004;Costa et al 2007).…”

Section: Microorganisms In Organically Grown Horticultural Cropsmentioning

confidence: 99%

Advances and Trends in Organic Fruit and Vegetable Farming Research

Dorais

Alsanius

2015

Horticultural Reviews: Volume 43

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“…ciceris in chickpeas and found more pronounced disease control in plants treated with dual inoculation. Sylla et al (2013) also reported a high level of disease suppression on treatment with multiple strains ( B. subtilis FZB24 and T. harzianum T58) against strawberry powdery mildew. This synergism can be attributed to the fact that the biocontrol agents probably use different mechanisms of biocontrol and therefore complement each other.…”

Section: Discussionmentioning

confidence: 95%

Enhanced Biocontrol of Cucumber Fusarium Wilt by Combined Application of New Antagonistic Bacteria Bacillus amyloliquefaciens B2 and Phenolic Acid-Degrading Fungus Pleurotus ostreatus P5

Wang

Cai

et al. 2021

Front. Microbiol.

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Continuous monoculture of cucumber (Cucumis sativus L.) typically leads to the frequent incidence of Fusarium wilt caused by Fusarium oxysporum f. sp. cucumerinum (FOC). As potent allelochemicals, phenolic acids are believed to be associated with soilborne diseases. This study aimed to investigate the effect of single or co-inoculation of antagonistic bacteria Bacillus amyloliquefaciens B2 and phenolic acid-degrading fungus Pleurotus ostreatus P5 on the suppression of cucumber Fusarium wilt. The strain B2 was identified as B. amyloliquefaciens based on biochemical, physiological, and 16S rDNA and gyrB gene sequence analyses. Strain B2 showed indole-3-acetic acid (IAA) and siderophore production and phosphate solubilization in in vitro assays. Scanning electron microscope (SEM) imaging showed the ability of strain B2 to adhere to the root surface of cucumber. P. ostreatus P5 could effectively degrade mixed phenolic acids as its sole source of carbon and energy for growth in liquid medium. In a pot experiment, four treatments were established as follows: (1) CK, uninoculated control; (2) B2, inoculation of strain B2; (3) P5, inoculation of strain P5; and (4) B2 + P5, co-inoculation of strain B2 and strain P5. At the end of the 60-day pot experiment, the B2, P5, and B2 + P5 treatments significantly reduced disease incidence by 48.1, 22.2, and 63.0%, respectively, compared to the CK treatment (p < 0.05). All three inoculation treatments significantly increased the growth of cucumber seedlings and suppressed the FOC population compared to the control (p < 0.05). High-performance liquid chromatography (HPLC) analysis showed that total phenolic acids were decreased by 18.9, 35.9, and 63.2% in the B2, P5, and B2 + P5 treatments, respectively. The results from this study suggest that combined application of B. amyloliquefaciens B2 and P. ostreatus P5 could be a promising strategy for suppressing Fusarium wilt and improving plant growth of cucumber seedlings under continuous cropping conditions.

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In vitro compatibility of microbial agents for simultaneous application to control strawberry powdery mildew (Podosphaera aphanis)

Cited by 20 publications

References 32 publications

Control of Botrytis cinerea in strawberries by biological control agents applied as single or combined treatments

Control of Botrytis cinerea in strawberries by biological control agents applied as single or combined treatments

Advances and Trends in Organic Fruit and Vegetable Farming Research

Enhanced Biocontrol of Cucumber Fusarium Wilt by Combined Application of New Antagonistic Bacteria Bacillus amyloliquefaciens B2 and Phenolic Acid-Degrading Fungus Pleurotus ostreatus P5

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