In-vitro comparison release study of novel liposome and conventional formulation containing Rosmarinus officinalis extract

Abstract

The purpose of this research article was to examine the impact of conventional Rosmarinus officinalis (without delivery systems) extract and extract incorporated liposomal delivery system on skin permeation velocity in vitro conditions was to determine whether there was a significant difference (in terms of duration) over the determined parameters.The environment condition was 47 mm diameter with a capacity of 20 ml in a water bath applied to the membrane surface in 37 ° C and using a double walled franz diffusion cell containing a syntetic polycarbonate membrane filter. 1., 3., 5., 10., 20., 40. in minutes and upto 160 minutes 50 rpm rotation speed (mimicking the application speed obtained from the skin) with classical methods and delivery named by calculating the transition rates of Rosmarinus officinalis extracts obtained by the alternative method through the diffusion cell.As a result of the study, it was clearly determined that the mixture of Rosemary (Rosmarinus officinalis) extract in the liposomal carrier system at a volume ratio of 1: 1 had an in vitro release rate of around 30% compared to 100% in 40 minutes, respectively, according to the traditional formula.

Antiproliferative and Antimicrobial Effects of Rosmarinus officinalis L. Loaded Liposomes

“…Carnosic acid and carnosol were the majority diterpenic phenols, and flavonoids were represented by apigenin, chrysin, rutoside, hyperoside, and luteolin-7-O-glucoside. The obtained results provide further arguments that confirm the presence of these compounds in the composition of the species, especially rosmarinic, caffeic, and chlorogenic acids, which were previously identified [1,9,12,23,39]. Carnosic and carnosol were also previously identified as majority compounds in the leaves of rosemary [9,12,21,23,39,40].…”

“…The characterization of the proposed liposomal formulations (Table 1) evidenced the achievement of good delivery systems that are in agreement with previous reports [35,36], recommending them for the assessment of polyphenolic content and their antiproliferative and antimicrobial potentials. Other similar studies were based on encapsulating rosemary essential oil [16,20,21,30], but also on its extracts [23,[36][37][38]. To the best of our knowledge, the present study is among the few that describe the encapsulation of a leaf extract of the species, representing the only study that describes the obtention of special delivery systems aimed at enhancing the antimicrobial and antiproliferative potentials of polyphenols from its derived vegetal medicinal product.…”

“…The phenolic compounds that are responsible for the biological activities of R. officinalis belong to the class of phenolic diterpenes (carnosic acid and carnosol) and to the class of phenolic acids (rosmarinic acid) [4,22]. They are responsible for the antimicrobial, antioxidant, antiproliferative, hepatoprotective, and antihyperglycemic activities [2,4,10,22,23].…”

“…Liposomes have also proven to be effective in the encapsulation of the essential oil obtained from rosemary [21,29,30]. At the same time, these formulations have also proven to improve the release of R. officinalis extract [23] or to encapsulate its main compounds, especially polyphenols, by enhancing their biological potential and limiting their major disadvantages [31]. Through the encapsulation of R. officinalis extract in liposomal carriers, it was proven that these formulations may improve the limitation of using polyphenols.…”

“…Through the encapsulation of R. officinalis extract in liposomal carriers, it was proven that these formulations may improve the limitation of using polyphenols. Existing studies cite better penetration enhancement for topical delivery [23], a more sustained release [20], an increased bioavailability [30], or the enhancement of the biological activities [15], compared to conventional extract systems.…”