In vitro comparison of myometrial contractility induced by aglepristone-oxytocin and aglepristone-PGF2alpha combinations at different stages of the estrus cycle in the bitch

Gogny, Anne; Mallem, Yassine; Destrumelle, S.; Thorin, Chantal; Desfontis, Jean‐Claude; Gogny, Marc; Fieni, F.

doi:10.1016/j.theriogenology.2010.06.023

Cited by 16 publications

(13 citation statements)

References 28 publications

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“…Our finding of stronger PTGS2 protein signals in the longitudinal compared with the circular myometrial layer is new and may indicate different contractile abilities of the two myometrial layers. This has been shown by Gogny et al using canine non-pregnant uterine strips in organbath and confirming a stronger response of the longitudinal compared with the circular myometrial layer to PGF2α (Gogny et al 2010). In contrast to our findings, in pregnant rats, strongest myometrial staining was localized in the circular muscle layer, while only negligible staining was found in the longitudinal layer (Dong et al 1996).…”

Section: Discussionsupporting

confidence: 87%

Do uterine PTGS2, PGFS, and PTGFR expression play a role in canine uterine inertia?

et al. 2021

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The aetiology of primary uterine inertia (PUI), which is the most common cause of canine dystocia, is still not elucidated. Prostaglandins (PGs) play a crucial role in parturition. We hypothesized that the expression of prostaglandin endoperoxidase synthase 2 (PTGS2), PGF2α synthase (PGFS), and corresponding receptor (PTGFR) is altered in PUI. We investigated PTGS2, PGFS, and PTGFR mRNA expression, and PTGS2 and PGFS protein expression in interplacental (IP) and uteroplacental sites (UP) in bitches with PUI, obstructive dystocia (OD), and prepartum (PC). PTGS2, PGFS, and PTGFR mRNA expression did not differ significantly between PUI and OD (IP/UP). PTGFR ratio in UP was higher in PC than in OD (p = 0.014). PTGS2 immunopositivity was noted in foetal trophoblasts, luminal and superficial glandular epithelial cells, smooth muscle cells of both myometrial layers, and weakly and sporadically in deep uterine glands. PGFS was localized in luminal epithelial cells and in the epithelium of superficial uterine glands. PTGS2 and PGFS staining was similar between PUI and OD, while PGFS protein expression differed between OD and PC (p = 0.0215). For PTGS2, the longitudinal myometrial layer of IP stained significantly stronger than the circular layer, independent of groups. These results do not support a role for PTGS2, PGFS, and PTGFR in PUI. Reduced PGFS expression in IP during parturition compared with PC and the overall lack of placental PGFS expression confirm that PGFS is not the main source of prepartal PGF2alpha increase. The difference in PTGS2 expression between IP myometrial layers warrants further investigation into its physiological relevance.

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Section: Discussionsupporting

confidence: 87%

Do uterine PTGS2, PGFS, and PTGFR expression play a role in canine uterine inertia?

et al. 2021

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“…Ibuki, Haga, Muramatsu, Mizumoto, and Itoh () described uterine contractions in non‐pregnant bitches during anoestrus and dioestrus, and administration of OT stimulated the contractile activity. In vitro, oxytocin‐induced contractions are more evident in myometrial longitudinal than circular fibres in the uterus of non‐pregnant bitches (Gogny et al., ).…”

Section: Discussionmentioning

confidence: 99%

Oxytocin receptors in dioestrous and anoestrous canine uteri

Tamminen

Sahlin

Masironi

et al. 2016

Reprod Domestic Animals

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The aim of the study was to localize oxytocin receptors (OTR) and measure mRNA expression of OTR in the canine uterus with and without the influence of progesterone. Uterine samples were taken from nine anoestrous and eight dioestrous bitches during ovariohysterectomy. Histological changes were evaluated in haematoxylin and eosin (HE)-stained samples. Purified polyclonal antibody for OTR was used in immunohistochemistry to localize receptors in uterine layers. Relative mRNA concentration of OTR was evaluated with real-time PCR from full-thickness uterine samples taken from the middle horn and the body. Myometrial smooth muscle cells, endometrial luminal epithelium (LE) and deep and superficial glandular epithelium were positively stained for oxytocin receptors in non-pregnant animals. No significant difference in staining intensity was detected between uterine middle horn and body. However, the staining intensity of LE was significantly higher in dioestrous than in anoestrous uteri (p < .05). Leucocytes and endothelium of blood vessels were also positively stained for OTR. Real-time PCR showed no significant differences in OTR mRNA expression between the middle horn and the body of the uterus, or between anoestrous and dioestrous uterus. No correlation was noted between OTR mRNA expression and blood progesterone concentration. In conclusion, despite the apparent inactivity, the uterus of the non-pregnant bitch expresses OTR. The distribution or relative expression of OTR does not differ between uterine horn and body in dioestrus or anoestrus except in LE. LE may have more oxytocin-dependent activity during dioestrus than anoestrus.

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“…In the cases presented, the authors chose to use the administration of aglepristone alone [ 7 , 23 ], in order to avoid the adverse effects of cloprostenol, applying the modified scheme proposed by Contri et al [ 23 ] for the bitch. In the cited paper, the efficacy of the anti-progestin in monotherapy was explained with the possibility that blocking progesterone receptors enhances myometrial contractility in the presence of relatively low levels of the endogenous prostaglandin F2α [ 23 ], which is released because of the pathologic inflammatory conditions of the uterus [ 31 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of a Modified Administration Protocol for the Medical Treatment of Feline Pyometra

Attard

Bucci

Parrillo

et al. 2022

Veterinary Sciences

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Pyometra is a common uterine disease of dogs and cats, typical of the luteal phase. Traditionally, ovariohysterectomy was considered the elective treatment for pyometra, but in some cases, such as breeding subjects or patients with a high anesthesiologic risk, medical treatment is preferred. Aglepristone is a progesterone receptor blocker and its use proved to be effective for the medical treatment of pyometra in bitches and queens. The aim of this work is to report the effectiveness, in the feline species, of a modified aglepristone administration protocol. Five intact queens were referred to veterinary care centers for pyometra. Aglepristone (15 mg/kg) was administered at D0, D2, D5, and D8, as described by Contri and collaborators for dogs. An antibiotic treatment (marbofloxacin, 3 mg/kg) was associated, and uterine conditions were checked with regular ultrasonographic exams. The uterus returned to its normal condition 10 days after starting the treatment and no adverse effects were reported. After treatment, three queens had an uneventful pregnancy. Even if the treated group was restricted and homogeneous, the proposed modified protocol proved to be useful and promising for the medical treatment of pyometra in cats; further studies are planned to verify its effectiveness in the long-term prevention of recurrence.

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In vitro comparison of myometrial contractility induced by aglepristone-oxytocin and aglepristone-PGF2alpha combinations at different stages of the estrus cycle in the bitch

Cited by 16 publications

References 28 publications

Do uterine PTGS2, PGFS, and PTGFR expression play a role in canine uterine inertia?

Do uterine PTGS2, PGFS, and PTGFR expression play a role in canine uterine inertia?

Oxytocin receptors in dioestrous and anoestrous canine uteri

Effectiveness of a Modified Administration Protocol for the Medical Treatment of Feline Pyometra

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