In vitro comparison of embryonic myoblasts and myogenic cells isolated from regenerating adult rat skeletal muscle

Jones, Paul H.

doi:10.1016/0014-4827(82)90266-x

Cited by 30 publications

(5 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A given weight of soleus muscle provided 50% more satellite cells than did EDL. The yields of satellite cells from each muscle were equivalent to those obtained by other authors (Bischoff 1974;Jones 1982;Düsterhöft and Pette 1993).…”

Section: Satellite Cell Yields From Edl and Soleus Rat Musclesmentioning

confidence: 80%

“…Satellite cells dissociated from adult muscle and grown in culture proliferate and differentiate into myotubes, thus mimicking several early steps of myogenesis occurring in vivo during muscle growth and regeneration (Jones 1982;Allen et al 1984;Feldman and Stokdale 1991). Several authors have approached the question of the possible existence of intrinsically programmed satellite cells by analysing the expression of myosin isotypes by cultured satellite cells (Miller 1990;Hartley et al 1991;Hartley and Yablonka-Reuveni 1992;Stockdale 1992;Edom et al 1994).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Differential myogenicity of satellite cells isolated from extensor digitorum longus (EDL) and soleus rat muscles revealed in vitro

Lagord

Soulet

Bonavaud

et al. 1998

Cell and Tissue Research

View full text Add to dashboard Cite

Following muscle damage, fast- and slow-contracting fibers regenerate, owing to the activation of their satellite cells. In rats, crush-induced regeneration of extensor digitorum longus (EDL, a fast muscle) and soleus (a slow muscle) present different characteristics, suggesting that intrinsic differences exist among their satellite cells. An in vitro comparative study of the proliferation and differentiation capacities of satellite cells isolated from these muscles is presented there. We observed several differences between soleus and EDL satellite cell cultures plated at high density on gelatin-coated dishes. Soleus satellite cells proliferated more actively and fused into myotubes less efficiently than EDL cells. The rate of muscular creatine kinase enzyme appeared slightly lower in soleus than in EDL cultures at day 11 after plating, when many myotubes were formed, although the levels of muscular creatine kinase mRNA were similar in both cultures. In addition, soleus cultures expressed higher levels of MyoD and myogenin mRNA and of MyoD protein than EDL satellite cell cultures at day 12. A clonal analysis was also carried out on both cell populations in order to determine if distinct lineage features could be detected among satellite cells derived from EDL and soleus muscles. When plated on gelatin at clonal density, cells from both muscles yielded clones within 2 weeks, which stemmed from 3-15 mitotic cycles and were classified into three classes according to their sizes. Myotubes resulting from spontaneous fusion of cells from the progeny of one single cell were seen regardless of the clone size in the standard culture medium we used. The proportion of clones showing myotubes in each class depended on the muscle origin of the cells and was greater in EDL- than in soleus-cell cultures. In addition, soleus cells were shown to improve their differentiation capacity upon changes in the culture condition. Indeed, the proportions of clones showing myotubes, or of cells fusing into myotubes in clones, were increased by treatments with a myotube-conditioned medium, with phorbol ester, and by growth on extra-cellular matrix components (Matrigel). These results, showing differences among satellite cells from fast and slow muscles, might be of importance to muscle repair after trauma and in pathological situations.

show abstract

Section: Satellite Cell Yields From Edl and Soleus Rat Musclesmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Differential myogenicity of satellite cells isolated from extensor digitorum longus (EDL) and soleus rat muscles revealed in vitro

Lagord

Soulet

Bonavaud

et al. 1998

Cell and Tissue Research

View full text Add to dashboard Cite

show abstract

“…We know from the literature that satellite cells can differentiate into 'mosaic myotubes' by fusing with autologous myogenic cells in vitro (Cossu etal., 1980;Jones, 1982) or in vivo (Lipton & Schultz, 1979;Jones, 1979;Watt, 1982;Watt et al, 1982Watt et al, , 1984. Thus, satellite cells present in a mince are presumably able to fuse with autologous myogenic cells isolated from another muscle.…”

Section: Introductionmentioning

confidence: 94%

Implantation of autologous cells in minced and devitalized rat skeletal muscles

Ghins

Schoor

Maréchal

1986

J Muscle Res Cell Motil

View full text Add to dashboard Cite

Triceps surae of 3 week-old female Wistar rats were minced and orthotopically autografted. Thirty days later, the regenerates developed a force of 314 +/- 58 mN during an isometric maximal tetanus and the maximal area of the muscle tissue was 3.2 +/- 0.5 mm2 (n = 9) in a cross-section. At 60 days, their mean maximal isometric force was increased (1159 +/- 367 mN) as also were their maximal muscle cross-section area (6.8 +/- 0.7 mm2;n = 6). If the minces were devitalized by a freeze and thaw procedure, the regenerates neither contracted under electrical stimulation nor contained any muscle fibre. If devitalized minces were seeded with myogenic cells isolated from the contralateral triceps surae, orthotopically autografted and allowed to regenerate for 30 days, the regeneration was weak: 46 +/- 14 mN of force, 1.2 +/- 0.3 mm2 of muscle area in minces devitalized by cold (n = 7) and 23 +/- 8 mN of force, 0.2 +/- 0.1 mm2 of muscle area in minces devitalized by cold supplemented by heat (n = 8). If viable minces were seeded with myogenic cells, there was no improvement in the extent of regeneration: 275 +/- 83 mN of force, 2.0 +/- 0.6 mm2 of muscle area at 30 days (n = 7) and 738 +/- 191 mN of force, 5.6 +/- 0.9 mm2 of muscle area at 60 days (n = 5). Consequently, although it is possible to induce regeneration by grafting myogenic cells into a devitalized mince, this procedure has no effect when applied to a viable mince.

show abstract

“…These “satellite” cells were first identified by electron microscopy and subsequently shown by DNA‐labeling studies in several species to be the source of regenerated muscle 31–34 . After muscle injury, satellite cells recapitulate the embryonic muscle program by proliferating, fusing, and differentiating into myofibers 35,36 . Quiescent satellite cells do not express the bHLH myogenic factors that regulate embryonic myogenesis, but express all of them in culture, although in a different sequence than the one observed during muscle embryogenesis 37 .…”

Section: Stem Cells In Adult Mesodermal Tissuesmentioning

confidence: 99%

Stem cells in regenerative biology and medicine

Stocum

2001

Wound Repair Regeneration

View full text Add to dashboard Cite

Embryonic stem cells of the mammalian blastocyst give rise to all the tissue lineages that begin to emerge at gastrulation. They are pluripotent cells and can be propagated in vitro without loss of pluripotency. Many adult tissues harbor cells that do not complete their differentiation program. These cells serve as self-renewing stem cells whose normal fate is to regenerate site-specific tissue, in response to either physiological cell turnover or damage inflicted by injury or disease. Neural, muscle, and bone marrow stem cells possess developmental potency far greater than their normal lineage-restricted fate. The understanding of the biology of stem cells is leading us into an era of regenerative medicine. The growth potential and pluripotency of embryonic stem cells and the developmental plasticity of adult stem cells, particularly those of bone marrow, make them potentially useful for replacing tissues, via transplantation or construction of bioartificial tissues, that either do not regenerate naturally or are damaged beyond their natural capability for regeneration. In addition to these two ways of replacing tissue, a third strategy of regenerative medicine is to stimulate regeneration in vivo from resident stem cells. Before these approaches become clinical reality, however, a number of basic research issues must be resolved, including the revision of our concept of a regeneration-competent cell.

show abstract

In vitro comparison of embryonic myoblasts and myogenic cells isolated from regenerating adult rat skeletal muscle

Cited by 30 publications

References 9 publications

Differential myogenicity of satellite cells isolated from extensor digitorum longus (EDL) and soleus rat muscles revealed in vitro

Differential myogenicity of satellite cells isolated from extensor digitorum longus (EDL) and soleus rat muscles revealed in vitro

Implantation of autologous cells in minced and devitalized rat skeletal muscles

Stem cells in regenerative biology and medicine

Contact Info

Product

Resources

About