In Vitro Comparison of Chen Medium and Optisol-GS Medium for Human Corneal Storage

Nelson, Leif R.; Hodge, David O.; Bourne, William M.

doi:10.1097/00003226-200011000-00005

Cited by 40 publications

(30 citation statements)

References 22 publications

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“…In a toxicity trial of Optisol-GS, which contains GM at a concentration of 100 μg/ml, SEM studies of human corneas after 7 days of storage in the solution detected no toxicity. 27 In our study, a 200 μg/ml dose of GM resulted in some endothelial cell damage, although not severe. It may be of great benefi t if new corneal storage media could be developed that cause less damage to endothelial cells than do currently available products.…”

Section: Discussionsupporting

confidence: 43%

Comparison of the influence of intracameral gentamicin, gatifloxacin, and moxifloxacin on the corneal endothelium in a rabbit model

et al. 2010

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Section: Discussionsupporting

confidence: 43%

Comparison of the influence of intracameral gentamicin, gatifloxacin, and moxifloxacin on the corneal endothelium in a rabbit model

et al. 2010

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“…Optisol-GS is a storage medium normally used to preserve postmortem human donor corneal tissue for the purpose of transplantation 31,32. It maintains epithelial, stromal, and endothelial viability for up to 14 days after corneal extraction 31,32.…”

Section: Discussionmentioning

confidence: 99%

Potentiation of Femtosecond Laser Intratissue Refractive Index Shaping (IRIS) in the Living Cornea with Sodium Fluorescein

Nagy

Ding

et al. 2010

Invest. Ophthalmol. Vis. Sci.

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Purpose To assess the effectiveness of intratissue refractive index shaping (IRIS) in living corneas and test the hypothesis that it can be enhanced by increasing the two-photon absorption (TPA) of the tissue. Methods Three corneas were removed from adult cats and cut into six pieces, which were placed in preservative (Optisol-GS; Bausch & Lomb, Inc., Irvine, CA) containing 0%, 0.25%, 1%, 1.5%, or 2.5% sodium fluorescein (Na-Fl). An 800-nm Ti:Sapphire femtosecond laser with a 100-fs pulse duration and 80-MHz repetition rate was used to perform IRIS in each piece, creating several refractive index (RI) modification lines at different speeds (between 0.1 and 5 mm/s). The lines were 1 μm wide, 10 μm apart, and ~150 μm below the tissue surface. The RI change of each grating was measured using calibrated, differential interference contrast microscopy. TUNEL staining was performed to assess whether IRIS or Na-Fl doping causes cell death. Results Scanning at 0.1 mm/s changed the RI of undoped, living corneas by 0.005. In doped corneas, RI changes between 0.01 and 0.02 were reliably achieved with higher scanning speeds. The magnitude of RI changes attained was directly proportional to Na-Fl doping concentration and inversely proportional to the scanning speed used to create the gratings. Conclusions IRIS can be efficiently performed in living corneal tissue. Increasing the TPA of the tissue with Na-Fl increased both the scanning speeds and the magnitude of RI changes in a dose-dependent manner. Ongoing studies are exploring the use of IRIS to alter the optical properties of corneal tissue in situ, over an extended period.

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“…Nelson and associates compared Chen medium with Optisol-GS in their study. 8 Although corneas stored in Chen medium were found thicker during storage than those stored in Optisol-GS medium, they reported no difference between 2 media regarding endothelial cell loss or keratocyte density during storage.…”

Section: Discussionmentioning

confidence: 92%

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Yüksel

Uzunel²,

Küsbeci³

2016

Exp Clin Transplant

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Objectives: To evaluate the efficacy of Eusol-C as a corneal storage medium on the survival of donor endothelium. Materials and Methods: Twenty-seven corneas not suitable for transplant were included in this study. All donor corneas were stored in Eusol-C at 4°C. Daily donor corneal endothelial cell counting was performed with an eye bank specular microscope. All corneas were discarded after the study process. Results: Mean donor age was 51.3 ± 18.8 years (range, 25-94 y). The mean duration between death and corneal excision was 9.5 ± 6.7 hours (range, 3-23 h). Mean endothelial cell density was 2195 ± 383 cells/mm 2 at the beginning of the preservation (range, 1361-2899 cells/mm 2 ). Donor endothelial cell density was between 1500 to 2000 cells/mm 2 in 9 corneas, 2000 to 2500 in 11 corneas, 2500 to 3000 in 5, and higher than 3000 in 2 corneas at baseline. Mean endothelial cell density was found 1658 cells/mm 2 on the eighth day of storage, with a mean endothelial cell loss rate of 24.5%. Corneas stored 9 to 24 days in Eusol-C had a rate of endothelial cell damage of 3.1% per day. Conclusions: Although our results revealed a higher endothelial cell loss than previous reports, overall performance of Eusol-C in preserving the donor endothelium may be satisfactory for clinical use.

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In Vitro Comparison of Chen Medium and Optisol-GS Medium for Human Corneal Storage

Abstract: Corneas stored in CM were thicker during storage than those stored in OM. The two storage media did not differ with respect to endothelial cell loss during storage or to the percentage of TUNEL-positive cells or keratocyte density at the end of the storage period.

Cited by 40 publications

References 22 publications

Comparison of the influence of intracameral gentamicin, gatifloxacin, and moxifloxacin on the corneal endothelium in a rabbit model

Comparison of the influence of intracameral gentamicin, gatifloxacin, and moxifloxacin on the corneal endothelium in a rabbit model

Potentiation of Femtosecond Laser Intratissue Refractive Index Shaping (IRIS) in the Living Cornea with Sodium Fluorescein

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