In vitro comparison between antimicrobial and antibiofilm effects of Green Propolis and Baccharis dracunculifolia against Staphylococcus pseudintermedius isolate

Abstract

Staphylococcus pseudintermedius is the leading cause of canine pyoderma.Honeybee products are common to treat this and other types of infections. High average annual population loss of bees has been observed. This study evaluated antibacterial and antibiofilm profile of Green Propolis and Baccharis dracunculifolia against S. pseudintermedius and the chemical similarities among both. Ethanolic extracts were produced and chemically characterized. The isolates were subjected to treatment with the extracts in both planktonic and sessile forms. Green propolis minimum inhibitory concentration (MIC) was 0.156 mg / mL, and minimum bactericidal concentration (MBC) was 0.312mg / mL. Baccharis dracunculifolia extract MIC and MBC was 0.312mg / mL and 2.5 mg / mL, respectivelly. Both extracts reduced SD55 formation of biofilm at minimum inhibitory concentration and at 1/8 minimum inhibitory concentration. The results observed in relation to ED99, were similar for both extracts. Besides that, similar chemical indicators between both extracts, including the presence of Artepellin C, suggest that the Baccharis dracunculifolia extract could be an alternative to the Green Propolis extract in the treatment of staph infections.

Multiplicative Effects of Essential Oils and Other Active Components on Skin Tissue and Skin Cancers

“…Baccharis dracunculifolia (B. dracunculifolia) is a domestic plant which is widely used as an immunomodulator and an anti-bacterial and anti-diabetic agent, but it possesses many other properties [10,11]. Several studies have evaluated the medicinal actions of B. dracunculifolia, especially its anti-microbial effects [12][13][14]. Dos Santos et al reported the anti-inflammatory effects of B. dracunculifolia leaves extract on a paw edema model, which is induced by carrageenan and formalin [15].…”

Ethnomedicinal, phytochemical and pharmacological investigations of Baccharis dracunculifolia DC. (ASTERACEAE)

“…Hydroalcoholic extract (70%) of dried leaves showed antimicrobial activity against Staphylococcus pseudintermedius, with MIC of 0.312 mg/ml and MBC of 2.5 mg/ml, in addition to having the ability to inhibit biofilm formation in vitro (Barbosa et al, 2022). This activity may be related to phenolic acid derivatives and flavonoids (Barbosa et al, 2022). The commercial hydroalcoholic extract of B. dracunculifolia was obtained from Ciclo Farma Indústria Química Ltda (Serrana, SP, Brazil), with the majority of artepillin C (Figure 5), rutin, and caffeic acid (Supplementary Figure S4) has been evaluated by Timbé et al (2021), presenting antimicrobial activity against all tested bacteria, with the highest inhibition halo for B. cereus (20.5 mm).…”

“…Hydroalcoholic extract (70%) of dried leaves showed antimicrobial activity against Staphylococcus pseudintermedius , with MIC of 0.312 mg/ml and MBC of 2.5 mg/ml, in addition to having the ability to inhibit biofilm formation in vitro ( Barbosa et al, 2022 ). This activity may be related to phenolic acid derivatives and flavonoids ( Barbosa et al, 2022 ).…”

“… Barbosa et al (2022) evaluated the hydroalcoholic extract (70%) of green propolis. They found antimicrobial activity against Staphylococcus pseudintermedius , with MIC of 0.156 mg/ml and MBC of 0.312 mg/ml, with a chemical composition similar to the extract of the leaves of B. dracunculifolia (derived from phenolic acids and flavonoids) ( Barbosa et al, 2022 ).…”

“…Research carried out with B. dracunculifolia indicates biological activities such as anti-inflammatory ( Santos et al, 2010 ; Brandenburg et al, 2020 ; França et al, 2022 ), antiulcerogenic ( Klopell et al, 2007 ; Lemos et al, 2007 ; Massignani et al, 2009 ; Costa et al, 2019 ; Boeing et al, 2021 ), immunomodulatory ( Burgos et al, 2022 ), neuroprotective ( França et al, 2022 ), antioxidant ( Veiga et al, 2017 ; Casagrande et al, 2018 ; Tomazzoli et al, 2021 ; Luchesi et al, 2022 ; Monteiro et al, 2022 ); antimicrobial ( Veiga et al, 2017 ; Casagrande et al, 2018 ; Salazar et al, 2018 ; Cazella et al, 2019 ; Pedrotti et al, 2019 ; Bonin et al, 2020 ; Timbé et al, 2021 ; Barbosa et al, 2022 ; Bernardes et al, 2022 ; Monteiro et al, 2022 ), anti-phytopathogenic ( Luchesi et al, 2022 ), insecticide and acaricide ( Chaaban et al, 2018 ; Cazella et al, 2020 ).…”