In vitro Comparative Evaluation of the Antibacterial and Antifungal Activities of Different Root Canal Sealers against Endodontic Pathogens

Ramachandra, Praveen KM; Krishnegowda, Sahadev C; Jaganath, Bharath Makonahalli; Rudranaik, Sandeep; Manjula, CG; Kurup, Nisha B; Madanan, Sajin

doi:10.5005/jp-journals-10052-0058

Cited by 4 publications

(4 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Este cemento no libera formaldehído en su proceso de polimerización, mejorando así su biocompatibilidad, pero disminuyendo relativamente su actividad antimicrobiana. (Asawaworarit et al;Ramachandra et al, 2016). El cemento sellador a base de hidróxido de calcio (Sealer 26), obtiene su actividad antimicrobiana debido a la separación de los iones hidroxilo y calcio, alterando el gradiente de pH de la membrana citoplásmica microbiana, produciendo efectos como la desnaturalización de las proteínas, (por la elevada concentración de iones hidroxilo sobre las proteínas), biosíntesis de lípidos, daño del ADN y de la membrana citoplasmática microbiana (Pérez et al;Mohammadi et al, 2011).…”

Section: Discussionunclassified

“…Se estima que el fracaso del tratamiento endodóntico puede alcanzar entre el 14 al 18% de los casos y puede ocurrir inclusive desde los seis meses post tratamiento y hasta luego de 10 años (Solanki et al, 2019). Su ocurrencia está asociada a una variedad de factores como la persistencia de bacterias (intracanal y extracanal), llenado inadecuado del canal, sobre extensión en la obturación, sellado coronal inadecuado, canales accesorios no tratados, diseño deficiente de la cavidad de acceso y complicaciones en la instrumentación (escalones, perforaciones o instrumentos separados) (Toledo et al, 2016;Tabassum & Khan, 2016;Yamaguchi et al, 2018).…”

Section: Introductionunclassified

See 1 more Smart Citation

Efecto Antimicrobiano de Tres Cementos Selladores Endodónticos frente a Streptococcus mutans, Enterococcus faecalis y Candida albicans

Chambilla-Torres

Sánchez-Tito

2021

Int. J. Odontostomat.

View full text Add to dashboard Cite

RESUMEN:El objetivo del estudio fue evaluar el efecto antimicrobiano de tres cementos selladores endodónticos frente a Streptococcus mutans, Enterococcus faecalis y Candida albicans. Se usó el método de difusión en agar, realizando pozos de 5 mm de diámetro por 4 mm de profundidad, en donde se colocaron los diferentes cementos que fueron preparados según las indicaciones del fabricante. Para S. mutans y E. faecalis se empleó agar cerebro-corazón, mientras que para C. albicans se empleó agar tripticasa soya como medios de cultivo. Las placas fueron incubadas por 48 horas a 37 ºC. Los halos de inhibición de crecimiento microbiano se midieron con un compás Vernier. Los resultados mostraron diferencias significativas entre todos los grupos p < 0,05. El cemento a base de óxido de zinc y eugenol (Endofill) mostró el mejor efecto antimicrobiano contra las tres cepas con halos de inhibición de 23,283 ± 5,497 mm para S. mutans, 8,468 ± 0,755 mm para E. faecalis y 21,965 ± 1,187 mm para C. albicans. Mientras que el cemento a base de resina (Vioseal) tuvo una actividad similar a Endofill solo para S. mutans (21,262 ± 2,602 mm) (p > 0,05), siendo bastante inferior para E. faecalis (6,642 ± 0,659 mm) y C. albicans (8,781 ± 0,735 mm). El cemento a base de hidróxido de calcio (Sealer 26) fue más efectivo frente a S. mutans (13,010 ± 2,006 mm) que ante E. faecalis (6,165 ± 0,978 mm) y C. albicans (5,640 ± 0,280 mm). Como conclusión, el cemento a base de óxido de zinc y eugenol (Endofill) mostró el mejor efecto antimicrobiano contra las tres cepas, seguido de los cementos a base de resina (Vioseal) y finalmente hidróxido de calcio (Sealer 26).

show abstract

Section: Discussionunclassified

Section: Introductionunclassified

Efecto Antimicrobiano de Tres Cementos Selladores Endodónticos frente a Streptococcus mutans, Enterococcus faecalis y Candida albicans

Chambilla-Torres

Sánchez-Tito

2021

Int. J. Odontostomat.

View full text Add to dashboard Cite

show abstract

“…After different periods of exposure, the zones of inhibition diameter were measured because the diffusion of a critical inhibitory concentration of the antibacterial agent into the agar media and the bacteria meeting a particular density were time related, altering the size of zone of inhibition. 17 …”

Section: Discussionmentioning

confidence: 99%

Comparison of Antimicrobial Efficacy of Four Different Plant Extracts against Cariogenic Bacteria: An In Vitro Study

Rai

Tripathi

Saha

et al. 2020

International Journal of Clinical Pediatric Dentistry

View full text Add to dashboard Cite

A bstract Background Dental caries is an oral disease which is infectious in nature associated with various pathogenic microorganisms and is prevalent across the world. Aim To evaluate and compare the anticariogenic properties of the different plant extracts against various cariogenic microorganisms. Materials and methods In phase I, the anticariogenic efficacy of four different herbal extracts, namely Ocimum sanctum ( Tulsi ), Terminalia chebula ( Harad ), Tinospora cordifolia ( Guduchi ), and Glycyrrhiza glabra (Licorice), was evaluated against two strains of bacteria viz. Streptococcus mutans and Lactobacillus acidophilus. In the second phase of the study, the overall decay depth was compared. Results In phase I, zone of inhibition was significantly higher in G. glabra (Licorice) followed the descending order by O. sanctum ( Tulsi ), T. c hebula ( Harad ), and T. cordifolia ( Guduchi ), respectively. In the second phase, it was found that the decay depth was significantly higher in T. cordifolia ( Guduchi ) followed the descending order by T. chebula ( Harad ), O. sanctum ( Tulsi ), and G. glabra (Licorice), respectively. Conclusion Glycyrrhiza glabra (Licorice) extract had potent antibacterial efficacy against S. mutans and L. acidophilus. Clinical significance Synthetic drugs have many adverse effects, so more attention has been paid to natural remedies because they are safe and effective. Today, the main interest is shifted toward the drugs which is derived from plants, which has led to the screening of many herbal plants for their potential antimicrobial activity and the same can be used clinically as an alternative of synthetic drugs used in dentistry. How to cite this article Rai A, Tripathi AM, Saha S, et al . Comparison of Antimicrobial Efficacy of Four Different Plant Extracts against Cariogenic Bacteria: An In Vitro Study. Int J Clin Pediatr Dent 2020;13(4):361–367.

show abstract

“…Ramachandra et al . [ 11 ] have demonstrated that AH Plus sealer showed greater zone of inhibition for C. albicans than Enterococcus faecalis and Escherichia coli , whereas some studies concluded that AH Plus sealer had no antimicrobial action. [ 12 13 ]…”

Section: Discussionmentioning

confidence: 99%

In vitro comparative evaluation of antifungal efficacy of three endodontic sealers with and without incorporation of chitosan nanoparticles against Candida albicans

2019

View full text Add to dashboard Cite

Aim: The aim of this study was to compare the antifungal efficacy of three endodontic sealers (AH Plus, Apexit Plus, and MTA Fillapex) with and without the incorporation of chitosan nanoparticles against Candida albicans. Materials and Methods: The present study was carried out by the Kirby–Bauer method. C. albicans were cultured in Sabouraud Dextrose Agar plates. Filter papers ( n = 10) were placed in the cultured Petri dishes and the sealers were mixed according to the manufacturer's instructions and placed on the top of the filter papers. Group division of sealers is as follows: Group I – AH Plus, Group II – Apexit Plus, and Group III – MTA Fillapex. Group IC, Group IIC, and Group IIIC were the addition of 2% chitosan nanoparticles with respective sealers. Plates were incubated for 18 h, and the zone of inhibition was measured with a measuring scale and values (in millimeter) were recorded. Statistical analysis was done by one-way analysis of variance followed by post hoc multiple pair-wise comparisons. Results: All the tested groups showed statistically significant difference ( P < 0.05) from each other. Two percent chitosan-incorporated groups showed superior zone of inhibition compared to sealers used alone. Group IC (16.35 ± 0.71 mm) had the highest zone of inhibition followed by Group I (13.8 ± 0.86 mm). For the remaining groups, the zone of inhibition was in the following order: Group IIC > Group II > Group IIIC > Group III. Conclusion: AH Plus sealer mixed with 2% chitosan showed significantly higher antifungal property. Mixing of 2% chitosan with endodontic sealer provides an added advantage so that endodontic re-infections can be minimized and will be helpful in retreatment cases.

show abstract

In vitro Comparative Evaluation of the Antibacterial and Antifungal Activities of Different Root Canal Sealers against Endodontic Pathogens

Cited by 4 publications

References 18 publications

Efecto Antimicrobiano de Tres Cementos Selladores Endodónticos frente a Streptococcus mutans, Enterococcus faecalis y Candida albicans

Efecto Antimicrobiano de Tres Cementos Selladores Endodónticos frente a Streptococcus mutans, Enterococcus faecalis y Candida albicans

Comparison of Antimicrobial Efficacy of Four Different Plant Extracts against Cariogenic Bacteria: An In Vitro Study

In vitro comparative evaluation of antifungal efficacy of three endodontic sealers with and without incorporation of chitosan nanoparticles against Candida albicans

Contact Info

Product

Resources

About