In vitro collection methods for<i>Malus</i>shoot cultures used for developing a cryogenic bank in Kazakhstan

Ромаданова, Н.В.; Mishustina, S.A.; Matakova, G.N.; Кушнаренко, С. В.; Rakhimbaev, I. R.; Reed, Barbara M.

doi:10.17660/actahortic.2016.1113.40

Cited by 11 publications

(11 citation statements)

References 4 publications

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“…These recent cases emphasize the importance of explant disinfection and contamination, even though many disinfection protocols are already available. Romadanova et al (2016b) used 523 detection medium (Viss et al 1991) to detect fungal and bacterial contamination in the in vitro culture of apple cultivars, clonal rootstocks and wild M. sieversii (Ledeb. M.…”

Section: Explant Infection and Disinfection And Wounding-induced Bromentioning

confidence: 99%

“…Shoot tip cryotherapy can be used to eradicate viruses from apple. In a study by Romadanova et al (2016b), shoot tips were excised from cold-hardened (22 °C for 8 h/day, then − 1 °C for 16 h/night, for 3 weeks) in vitro shoots and subjected to vitrification cryotherapy, as described by Reed and Yu (1995). Virus-free frequencies were 60-100% for ACLSV in six of the nine genotypes, 25-67% for ASPV in five of the six genotypes, 50% for ASGV in one genotype and 89% for ApMV in one genotype.…”

Section: Cryotherapymentioning

confidence: 99%

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In vitro tissue culture of apple and other Malus species: recent advances and applications

Silva

Gulyás

Magyar-Tábori

et al. 2019

Planta

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Main conclusion Studies on the tissue culture of apple have allowed for molecular, biotechnological and applied breeding research to advance. In the past 8 years, over 100 papers advancing basic biology, genetic transformation and cryobiology have emerged. Apple (Malus × domestica Borkh.; Rosaceae) is an important fruit crop grown mainly in temperate regions of the world. In vitro tissue culture is a biotechnological technique that has been used to genetically improve cultivars (scions) and rootstocks. This updated review presents a synthesis of findings related to the tissue culture of apple and other Malus spp. between 2010 and 2018. Increasingly complex molecular studies that are examining the apple genome, for example, in a bid to identify the cause of epigenetic mutations and the role of transposable elements in this process would benefit from genetically stable source material, which can be produced in vitro. Several notable or curious in vitro culture methods have been reported to improve shoot regeneration and induce the production of tetraploids in apple cultivars and rootstocks. Existing studies have revealed the molecular mechanism underlying the inhibition of adventitious roots by cytokinin. The use of the plant growth correction factor allows hypothetical shoot production from leaf-derived thin cell layers relative to conventional leaf explants to be determined. This updated review will allow novices and established researchers to advance apple and Malus biotechnology and breeding programs.

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Section: Explant Infection and Disinfection And Wounding-induced Bromentioning

confidence: 99%

Section: Cryotherapymentioning

confidence: 99%

In vitro tissue culture of apple and other Malus species: recent advances and applications

Silva

Gulyás

Magyar-Tábori

et al. 2019

Planta

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“…M. Roem. The collection was obtained in the period from 2002 to 2020 from field plants provided by the Kazakh Research Institute of Fruit Growing and Viticulture, "Liza" Farm, "Suzdaleva" Farm, "Integration-Turgen" LLP, wild forms were collected from the Main Botanical Garden (MBG) and from various areas of the Ile-Alatau National Park [13].…”

Section: Methodsmentioning

confidence: 99%

“…Plants were propagated in vitro on Murashige-Skoog (MS) medium [14] containing 30 g/L sucrose, 0.5 mg/L 6-benzylaminopurine (BAP), 0.01 mg/L indolylbutyric acid (IBA), 4.0 g/L Plant TC agar (Phytotechnology Laboratories®, Lenexa, KS), 1.25 g/L Gelrite™ (Phytotechnology Laboratories®), pH 5.7. All cultures were grown in glass culture vessels (237 mL) (Phytotechnology Laboratories®) in a growth room at 24°C±1°C with 16 h photoperiod of white fluorescent lights at 40 µM m -2 s -1 (standard growth room conditions) with two types of OPPLE tubular fluorescent lamps: YK 21RR 16/G 21W 6500K RGB and YK 21RL 16/G 21W 4000K RGB supplied by ElectroComplex in Corporation, Almaty, Republic of Kazakhstan (http://elcor.kz) [13]. Plants were subcultured in vitro every 3-4 weeks.…”

Section: Methodsmentioning

confidence: 99%

Chemotherapy of apple shoots in vitro as method of viruses eradication

Ромаданова

Толеген

Köken

et al. 2021

ijbch

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Studies carried out in Kazakhstan and abroad have shown the widespread prevalence of viral, bacterial and fungal diseases on apple commercial varieties and clonal rootstocks. Current paper presents the results on the viruses eradication from in vitro shoots of varieties and clonal rootstocks of apple (Malus domestica Borkh.) using chemotherapy and obtaining healthy super-elite planting stocks. Ribavirin at concentrations of 75 and 100 mg/L caused severe in vitro shoot necrosis. Three subcultures on Murashige-Skoog medium with 50 mg/L ribavirin was efficient for elimination of Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), and Apple mosaic virus (ApMV) from in vitro shots of Malus varieties and clonal rootstocks. A virus-free in vitro collection (42 accessions) was established, which was used for create a cryobank of shoot tips at -196°C and to obtain virus-free planting stocks. The percentage of in vitro shoots rooting ranged from 50% to 90%. The survival rate of in vitro shoots rooted in the soil substrate is more than 90%.

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“…has been established and is constantly being updated. A list of cultures in the collection was included in the Botanic Gardens Conservation International (BGCI) database (Romadanova et al 2016a). Cryopreservation is not only used for the long-term preservation of the plant genetic diversity in Kazakhstan, but also for the recovery of crops from phytopathogens (Kushnarenko et al 2017;Romadanova et al 2016b).…”

Section: Conservation and Study Of Genetic Resources Of The Kazakhstamentioning

confidence: 99%

Current status and prospects of plant biotechnology in Kazakhstan

Zhambakin

Zhapar

2020

Plant Biotechnol Rep

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Biotechnological methods are becoming an integral part of biological research. This review presents some of the most significant scientific results of Kazakhstan biologists in the field of plant biotechnology over the past 10 years. One of the recent important areas of application of biotechnological methods is the conservation and study of plant genetic resources and bioremediation. Studies on the flora lead to the identification of new sources of previously unknown biologically active materials, especially among wild plants growing in Kazakhstan. In addition, various biotechnological approaches are used to increase the efficiency of breeding practices for the production of new crop varieties.

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In vitro collection methods forMalusshoot cultures used for developing a cryogenic bank in Kazakhstan

Cited by 11 publications

References 4 publications

In vitro tissue culture of apple and other Malus species: recent advances and applications

In vitro tissue culture of apple and other Malus species: recent advances and applications

Chemotherapy of apple shoots in vitro as method of viruses eradication

Current status and prospects of plant biotechnology in Kazakhstan

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