In vitro clonal propagation through mature nodes of Tinospora cordifolia (Willd.) Hook. F. &amp; Thoms.: An important ayurvedic medicinal plant

Raghu, A. V.; Geetha, S.; Martin, Gerald; Balachandran, Indira; Ravindran, P. N.

doi:10.1079/ivp2006824

Cited by 41 publications

(25 citation statements)

References 8 publications

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“…Early workers reports same difficulty for micropropagtion of this species [13,18]. PG effectively controlled tissue browning in T. cordifolia in present investigation.…”

Section: Discussionsupporting

confidence: 68%

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Phloroglucinol Plays Role in Shoot Bud Induction and In Vitro Tuberization in Tinospora Cordifolia- A Medicinal Plant with Multi- Therapeutic Application

Kumar¹

2015

Adv Tech Biol Med

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This paper shows the potential of phloroglucinol for In Vitro regeneration and tuberization of microshoots of Tinospora cordifolia. Within a week of nodal explants culture, axillary bud in nodal segments sprouted in all the media investigated including basal MS media without any growth regulators. Maximum response (52.2 per cent) evinced by MS media supplemented with 6.98 µM Kin. The same media produced maximum response for multiple shoot formation out of total shoot bud sprouted fostering average 3 shoots with 3.9 cm shoot length and 4.2 number of leaves per shoot. Effect of PG also investigated for mass multiplication and In Vitro rooting. Basal MS + 6.98 µM Kin + 79.4 µM PG increased shoot bud induction from 52.2 per cent to 84.8 per cent and multiple shoot production per cent from 12.9 to as high as 60.3 percent and also supported maximum number of shoot per explants (7.5). The same treatment promoted highest axillary shoot proliferation in terms of shoot length (3.9 cm) and number of leaves per shoot (4.3). Phloglucinol induced In Vitro tuberization in microshoots. Maximum rooting response observed in ½ MS medium supplemented with 7.4 µM IBA and 793.7 µM PG with 81.1 culture responding taking just 8.7 days to sprout and producing maximum number of roots (3.2) with an average length (3.1 cm). Rooted explants successfully hardened in Soil: Vermicompost: Vermiculite (1:1:1) with 100% success.

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“…Early workers reports same difficulty for micropropagtion of this species [13,18]. PG effectively controlled tissue browning in T. cordifolia in present investigation.…”

Section: Discussionsupporting

confidence: 68%

“…Micropropagation may help in propagation and conservation of this plant. Culture of shoot meristems, especially through enhanced axillary branching, permits rapid propagation of certain plants and a high degree of genetic uniformity of the progeny [13,14].…”

Section: Introductionmentioning

confidence: 99%

Phloroglucinol Plays Role in Shoot Bud Induction and In Vitro Tuberization in Tinospora Cordifolia- A Medicinal Plant with Multi- Therapeutic Application

Kumar¹

2015

Adv Tech Biol Med

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“…Similar to our findings, they also observed induction of single shoot by higher concentrations of BAP. However, Raghu et al (2006) reported benzyl adenine to be effective than Kn for auxiliary shoot proliferation while Kn is better for shoot elongation. Cytokinins are one of the most important hormones for shoot proliferation (Lane 1979;Bhojwani 1980).…”

Section: Discussionmentioning

confidence: 99%

<i>In vitro</i> study of <i>Tinospora cordifolia</i> (Willd.) Miers (Menispermaceae)

Singh¹,

Sah²,

Pradhan³

et al. 2010

Botanica Orientalis

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In vitro study was carried out in an important medicinal plant Tinospora cordifolia (Willd.) Miers belonging to the family Menispermaceae.Vegetative parts such as stem, leaf and nodal explants were excised from an elite in vivo grown mature plant and thereafter cultured on Murashige-Skoog (MS) medium supplemented with different hormonal concentrations for callus induction and organogenesis. Callus formation occurred from nodal segments, leaf and inter-node explants when planted on different combinations of hormones. Tinospora cordifolia showed response for in vitro shoot growth from the nodal segment. The best shoot growth was observed on MS medium supplemented with kinetin (1.5 mg/l). Similarly, the best result for root induction was obtained on MS medium supplemented with 6-benzylaminopurine (1.0 mg/l) and naphthaleneacetic acid (2.5 mg/l).

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“…Callus induction and organogenesis has been reported from cultured vegetative parts such as stem, leaf and nodal explants from an elite in vivo grown mature plant of Tinospora cordifolia on MS medium supplemented with different hormonal concentrations [7]. In vitro propagation in T. cordifolia has been reported from nodal segments through axillary shoot proliferation [8,9]. Media composition plays a key role in morphogenesis as nutritional requirement for optimal growth of a tissue under in vitro conditions varies with species [10].…”

Section: Discussionmentioning

confidence: 99%

“…DOI: 10.9790/264X-03036569 www.iosrjournals.org 69 | Page benzyladenine (BA) has been found to be more effective than Kinetin for axillary shoot proliferation in T. cordifolia [8]. BAP combined with auxins exhibit synergistic effect and hence has also been used by number of researchers [11].…”

Section: In Vitro Conservation Of Tinospora Formanii Udayan and Pradementioning

confidence: 99%

In Vitro Conservation of Tinospora formanii Udayan and Pradeep-A Rare Endemic Plant from Western Ghats

P¹,

Bindu²,

Udayan³

et al. 2017

IOSR JBB

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In vitro clonal propagation through mature nodes of Tinospora cordifolia (Willd.) Hook. F. & Thoms.: An important ayurvedic medicinal plant

Cited by 41 publications

References 8 publications

Phloroglucinol Plays Role in Shoot Bud Induction and In Vitro Tuberization in Tinospora Cordifolia- A Medicinal Plant with Multi- Therapeutic Application

Phloroglucinol Plays Role in Shoot Bud Induction and In Vitro Tuberization in Tinospora Cordifolia- A Medicinal Plant with Multi- Therapeutic Application

<i>In vitro</i> study of <i>Tinospora cordifolia</i> (Willd.) Miers (Menispermaceae)

In Vitro Conservation of Tinospora formanii Udayan and Pradeep-A Rare Endemic Plant from Western Ghats

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