In vitro clonal propagation and regeneration of the commercially important plant Mexican oregano (Lippia graveolens)

Evaluation of Somaclonal and Ethyl Methane Sulfonate-Induced Genetic Variation of Mexican Oregano (Lippia graveolens H.B.K.)

“…The cultivation of nodal explants in MS medium supplemented with 0.5 mg L −1 of BA resulted in averages of 3. The average number of shoots obtained from axillary buds by adding BA to the medium, was higher than that reported by Castellanos-Hernández et al [14], who describe an average of 2.8 shoots per explant, using the same conditions. In addition, that report only showed activation of buds, in comparison with the results presented here.…”

“…The number of shoots generated in each stage of micro-propagation of the three genotypes did not present significant changes, although the length of the shoots decreased in successive propagations. The number of shoots can be increased in each number of sub-cultivations or kept The average number of shoots obtained from axillary buds by adding BA to the medium, was higher than that reported by Castellanos-Hernández et al [14], who describe an average of 2.8 shoots per explant, using the same conditions. In addition, that report only showed activation of buds, in comparison with the results presented here.…”

“…al. [14]. The nodal segments were obtained from three plants germinated in vitro, designated B, C, and D (mother plants), which were derived from collection A.…”

“…The use of cytokinins, such as BA, in the proliferation of axillary buds by nodal segments has been reported in several propagation studies [13,14,25]. Islam et al [26] report the use of BA at a concentration of 0.05 mg L −1 in the in vitro propagation of Vitex negundo L., showing production of multiple shoots.…”

“…The application of tissue culture techniques has allowed the massive propagation of plants under aseptic and regulated conditions [13]. The regeneration of Mexican oregano has been developed through the in vitro establishment of leaves, with callus formation, and through axillary bud multiplication [14].…”