In vitro clonal analysis of murine pluripotent stem cells isolated from skeletal muscle and adipose stromal cells

Case, Jamie; Horváth, Tamara; Ballas, Christopher B.; March, Keith L.; Srour, Edward F.

doi:10.1016/j.exphem.2007.09.003

Cited by 22 publications

(18 citation statements)

References 46 publications

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“…In accord with several previous studies [24,25], the current study confirmed the mesenchymal nature (CD29-, CD90-positive expression) and non-hematopoietic, non-endothelial origin of the cells, based on their lack of cell surface expression of CD45, CD34 and CD31. The fibroblast-like spindle morphology, cell surface epitopes, and capacity for multilineage mesenchymal differentiation confirmed that the cells used in this study were pMDSCs.…”

Section: Discussionsupporting

confidence: 77%

Inhibition of adipogenic differentiation by myostatin is alleviated by arginine supplementation in porcine-muscle-derived mesenchymal stem cells

Lei

Yang

et al. 2011

Sci. China Life Sci.

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Porcine mesenchymal stem cells in postnatal muscle have been demonstrated to differentiate into adipocytes. This increases adipocyte number and lipid accumulation, and is thought to be the origin of intramuscular fat. In this study, the effects of myostatin and arginine on adipogenic differentiation in mesenchymal stem cells derived from porcine muscle (pMDSCs) were investigated in vitro. Intracellular triglyceride levels were reduced by exogenous myostatin and increased by arginine supplementation or myostatin antibody (P<0.01). The inhibition of lipid accumulation by myostatin in pMDSCs was alleviated by arginine supplementation (P<0.01). Expression patterns of adipogenic transcription factors showed that exogenous myostatin suppressed PPARγ2 and aP2 expression (P<0.01), while supplemental arginine or myostatin antibody promoted ADD1 expression (P<0.01). Furthermore, compared with the addition of either myostatin protein or antibody alone, ADD1 and PPARδ expression were promoted by the combination of arginine and myostatin (P<0.01), and arginine combined with myostatin antibody promoted the expression of ADD1, PPAR, C/EBP, PPAR2 and LPL in pMDSCs (P<0.05). These results suggest that myostatin inhibits adipogenesis in pMDSCs, and that this can be alleviated by arginine supplementation, at least in part, through promoting ADD1 and PPAR expression. myostatin, arginine, adipogenic differentiation, mesenchymal stem cells, porcine Citation:Lei H L, Yu B, Yang X R, et al. Inhibition of adipogenic differentiation by myostatin is alleviated by arginine supplementation in porcine-muscle-derived mesenchymal stem cells. Sci China Life Sci, 2011, 54: 908 -916,

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Section: Discussionsupporting

confidence: 77%

Inhibition of adipogenic differentiation by myostatin is alleviated by arginine supplementation in porcine-muscle-derived mesenchymal stem cells

Lei

Yang

et al. 2011

Sci. China Life Sci.

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“…Disagree with some authors [24] that demonstrated that mouse adipose tissue-derived stem cells do not express the OCT-4 markers and agree with Izadpanah et al [25] that showed that OCT4 and SOX2 are abundantly expressed in cultured rhesus and human AD-MSC, our results showed the presence of a significative expression of stemness marker genes, OCT4, SOX2 and NANOG. The study of gene expression levels, was performed on the basis of related mRNA levels, evaluated during five subsequent subcultures.…”

Section: Discussionsupporting

confidence: 80%

Gene Expression Analysis of Rat Adipose Tissue-Derived Stem Cells

Cannella¹,

Casella²,

Altomare³

et al. 2015

IJST

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“…Similarities in surface marker profiles, morphology, and differentiation potentials assayed in vitro led to suggestions that each of these multipotent cell types arise from a common adult progenitor cell (37) and then may adopt tissue-specific attributes according to its particular niche (38). Adipose tissue represents a rich source of such cells.…”

Section: Discussionmentioning

confidence: 99%

Adipocytes as Immune Cells: Differential Expression of TWEAK, BAFF, and APRIL and Their Receptors (Fn14, BAFF-R, TACI, and BCMA) at Different Stages of Normal and Pathological Adipose Tissue Development

Alexaki

Notas

Pelekanou

et al. 2009

The Journal of Immunology

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Adipose tissue represents a rich source of multipotent stem cells. Mesenchymal cells, isolated from this source, can differentiate to other cell types in vitro and therefore can be used for a number of regenerative therapies. Our view of adipose tissue has recently changed, establishing adipocytes as new members of the immune system, as they produce a number of proinflammatory cytokines (such as IL-6 and TNFα and chemokines, in addition to adipokines (leptin, adiponectin, resistin) and molecules associated with the innate immune system. In this paper, we report the differential expression of TNF-superfamily members B cell activating factor of the TNF Family (BAFF), a proliferation inducing ligand (APRIL), and TNF-like weak inducer of apoptosis (TWEAK) in immature-appearing and mature adipocytes and in benign and malignant adipose tissue-derived tumors. These ligands act through their cognitive receptors, BAFF receptor, transmembrane activator and calcium signal-modulating cyclophilic ligand (TACI), B cell maturation Ag (BCMA), and fibroblast growth factor-inducible 14 (Fn14), which are also expressed in these cells. We further report the existence of functional BCMA, TACI, and Fn14 receptors and their ligands BAFF, APRIL, and TWEAK on adipose tissue-derived mesenchymal cells, their interaction modifying the rate of adipogenesis. Our data integrate BAFF, APRIL, and TWEAK and their receptors BCMA, TACI, and Fn14 as novel potential mediators of adipogenesis, in addition to their specific role in immunity, and define immature and mature adipocytes as source of immune mediators.

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In vitro clonal analysis of murine pluripotent stem cells isolated from skeletal muscle and adipose stromal cells

Abstract: These data demonstrate that phenotypically defined PSCs remain functionally heterogeneous at the single-cell level and illustrate that morphologic lineage commitment may be independent of exclusive expression and/or loss of associated lineage specific genes.

Cited by 22 publications

References 46 publications

Inhibition of adipogenic differentiation by myostatin is alleviated by arginine supplementation in porcine-muscle-derived mesenchymal stem cells

Inhibition of adipogenic differentiation by myostatin is alleviated by arginine supplementation in porcine-muscle-derived mesenchymal stem cells

Gene Expression Analysis of Rat Adipose Tissue-Derived Stem Cells

Adipocytes as Immune Cells: Differential Expression of TWEAK, BAFF, and APRIL and Their Receptors (Fn14, BAFF-R, TACI, and BCMA) at Different Stages of Normal and Pathological Adipose Tissue Development

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