In vitro chromosome doubling potential of colchicine, oryzalin, trifluralin, and APM in Brassica napus microspore culture

Hansen, Nana Wesley; Andersen, S. B.

doi:10.1007/bf00032447

Cited by 104 publications

(76 citation statements)

References 14 publications

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“…Most importantly, cytoskeletal rearrangements themselves can trigger changes in development, since the utilization of colchicine or cytochalasin D is sufficient to induce embryogenesis in microspores (Zhao et al 1996;Gervais et al 2000). MT depolymerising substances such as colchicine and amiprophosmethyl, applied in order to interrupt mitosis and induce duplication of the haploid genome, have produced inductive effects on microspore embryogenesis in some genotypes of Brassica (Hansen and Andersen 1996;Zhou et al 2002), rice (Alemanno and Guiderdoni 1994), coffee (Herrera et al 2002) and wheat (Barnabás et al 1991;Soriano et al 2007). …”

Section: Introductionmentioning

confidence: 99%

Enhanced induction of microspore embryogenesis after n-butanol treatment in wheat (Triticum aestivum L.) anther culture

2008

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The aim of this study was the improvement of embryo production in wheat anther culture. Three butanol alcohols, n-butanol, sec-butanol and tert-butanol, were evaluated for their effect on microspore embryogenesis in two spring cultivars of wheat, Pavon and Caramba. Application of n-butanol, at 0.1 and 0.2% (v/v) in the induction media for five hours, highly improved embryo production in both cultivars. Sec-and tert-butanol performed similarly to control plates. Regeneration ability was unaffected by any butylalcohol treatment. As a consequence of the higher embryo production after n-butanol treatment, the number of green regenerated plants increased up to 5 times in cultivar Pavon and up to 3 times in cultivar Caramba. The percentage of green plants was improved or unaffected by the treatment. Doubled haploid plant production was between 2 and 4 times higher after n-butanol treatment than in control plates. Therefore, n-butanol was successfully applied in the production of wheat doubled haploids. This primary alcohol is known as an activator of phospholipase D and has been previously reported to disrupt cortical microtubules and detach them from the plasma membrane in plants. Its effects on androgenetic induction could confirm the importance of microtubule regulation in plant cell fate, specifically in microspore development. A possible implication of phospholipase D is discussed.

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Section: Introductionmentioning

confidence: 99%

Enhanced induction of microspore embryogenesis after n-butanol treatment in wheat (Triticum aestivum L.) anther culture

2008

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“…These values fall into the lower to middle range of the diploidization rates of 37-94 % reported in the literature for isolated microspores after colchicine treatment Möllers et al 1994;Iqbal et al 1994;Hansen and Andersen 1996;Klutschewski 2013). Three ISLs had significantly reduced diploidization rates of 23.9, 25.6 and 27.1 % respectively.…”

Section: Discussionmentioning

confidence: 91%

“…In addition, the diploidization rates of the two lines are quite similar to one another and to the rate of ER984, raising the possibility that just one factor, a gene linked to E44M50-147E, may be responsible for the reduced diploidization rates of the three lines. Then again, the broad range of diploidization rates from 37 to 94 % described in the literature Möllers et al 1994;Iqbal et al 1994;Hansen and Andersen 1996;Klutschewski 2013) would argue for a polygenic control of this trait. Taking into account the large genetic distance between the parents of the mapping populations, 'Express 617' being a natural biannual rapeseed type and 'RS239' an annual resynthesized rapeseed, it is quite likely that more than one gene controlling diploidization rates in rapeseed is segregating in our populations.…”

Section: Discussionmentioning

confidence: 99%

“…During the development of DH lines in rapeseed, diploization rates are increased by a treatment with colchicine, a natural alkaloid extracted mainly from the plant Colchicum autumnale, which depolymerises microtubules and disrupts regular mitotic cell division. Möllers et al 1994;Zhao et al 1996aZhao et al , 1996bHansen and Andersen 1996;Zhou et al 2002aZhou et al , 2002bWeber et al 2005;Seguí-Simarro and Nuez 2008). As discussed by Möllers and Iqbal (2009), the most efficient protocol for the colchicine treatment is a treatment of the freshly isolated microspores during the first days of cultivation, which will lead to entirely diploid plants with full fertility and diploidization rates of up to 90 %.…”

Section: Introductionmentioning

confidence: 99%

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Identification and evaluation of intervarietal substitution lines of rapeseed (Brassica napus L.) with donor segments affecting the diploidization rate of isolated microspores

et al. 2016

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In Brassica species microspore derived doubled haploid lines are an important tool in breeding and research. A limiting step in the production of doubled haploid lines is the diploidization of the microspores. Strong differences have been observed in diploidization rates between different genotypes but little is know about the genetic control of these differences. To identify genomic regions that carry genetic factors controlling the diploidization rate of isolated microspores of rapeseed, marker segregations were compared between segregating populations of diploid and haploid microspore derived embryos and a BC 1 from a cross between 'Express 617' and 'RS239'. After map construction ten intervarietal substitution lines from the same cross were selected with donor segments covering five genomic regions that showed a pattern of skewed marker segregations across the three populations indicative of the segregation of genes controlling the diploidization rates. The diploidization rates of microspores of the ten lines ranged from 23.9 to 58.7 % while the recurrent parent 'Express 617' showed a rate of 52.5 %. For three lines the diploidization rates were significantly lower (P = 0.05) than the rate of 'Express 617'. By comparing donor segments between the significant and the non-significant lines, seven genomic regions that cover just between 4.17 and 6.16 % of the rapeseed genome were identified that may contain genetic factors controlling diploidization rates in rapeseed. In addition, one marker was found that has a high probability to be linked to such a factor. The significant lines represent an ideal material for further in depth studies of this trait.

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“…Several microtubule depolymerising herbicides were also proved to be efficient for in vitro chromosome doubling of microspores. Zhao & Simmonds (1995) tested trifluralin and Hansen & Andersen (1996) trifluralin, oryzalin and amiprophos methyl in the spring rapeseed cultivar Topas. They achieved the mean rate of diploidization from 60% to 65%, which is comparable with the application of colchicine.…”

mentioning

confidence: 99%

Chromosome doubling effects of selected antimitotic agents in Brassica napus microspore culture

Klíma¹,

Vyvadilová²,

Kučera³

2008

CZECH J GENET PLANT

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Effects of microspore culture treatment with antimitotic agents colchicine, trifluralin and oryzalin on the frequency of embryo formation, embryo development, plant regeneration and diploidization rate in three F 1 hybrids of winter rapeseed cultivars were compared. The ploidy level analysis of 1709 flowering microspore-derived plants showed that in vitro applications of all antimitotic drugs increased the rate of doubled haploid (DH) plants significantly. The mean rate of DH plants from the trifluralin treatment was 85.7%, from colchicine 74.1% and 66.5% in the case of oryzalin, while only 42.3% in the untreated control variant whereas in vivo additional application of colchicine at the plantlet stage did not significantly increase the mean rate of DH plants (55.6%). Although there were no significant differences in diploidization efficiency between the in vitro applications of particular antimitotic agents, trifluralin showed to be the most suitable because of its positive effect on embryo development and conversion into whole plants. In addition, the diploidization rate was sufficient and stable in all genotypes tested. The results indicate that the trifluralin treatment of microspore cultures could provide efficient chromosome doubling for the production of doubled haploid lines from winter oilseed rape breeding materials.

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In vitro chromosome doubling potential of colchicine, oryzalin, trifluralin, and APM in Brassica napus microspore culture

Cited by 104 publications

References 14 publications

Enhanced induction of microspore embryogenesis after n-butanol treatment in wheat (Triticum aestivum L.) anther culture

Enhanced induction of microspore embryogenesis after n-butanol treatment in wheat (Triticum aestivum L.) anther culture

Identification and evaluation of intervarietal substitution lines of rapeseed (Brassica napus L.) with donor segments affecting the diploidization rate of isolated microspores

Chromosome doubling effects of selected antimitotic agents in Brassica napus microspore culture

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