In vitro characterization of two lineage-negative CD34+ cell-enriched hematopoietic cell populations from human UC blood

Flores-Guzmán, Patricia; Flores-Figueroa, Eugenia; Martı́nez-Jaramillo, Guadalupe; Mayani, Héctor

doi:10.1080/14653240500238228

Cited by 17 publications

(17 citation statements)

References 35 publications

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“…We have previously demonstrated that this selection system is effective in enriching for CD34 ϩ cells and CFCs, and that the selected cell fraction is devoid of stromal cells and the frequency of mature Lin ϩ cells is extremely low (8,9). In the present study CD34 ϩ cells corresponded to 55-75% of the selected cells, whereas the frequency of CFCs was 6-12% of selected cells.…”

Section: Primitive Cell Enrichmentmentioning

confidence: 49%

“…On the indicated days, CD34 ϩ cells were identified and quantified by immunocytochemistry, both before and after negative selection, as previously described (8,9).…”

Section: Cd34 ϩ Cell Quantificationmentioning

confidence: 99%

“…Primitive, Lin Ϫ cells, including those expressing the CD34 antigen (CD34 ϩ cells) were enriched from MNCs by negative selection (12), using the StemSep™ system (Stem Cell Technologies Inc; STI, Vancouver, Canada), as previously described in detail (8,9). We have previously demonstrated that this selection system is effective in enriching for CD34 ϩ cells and CFCs, and that the selected cell fraction is devoid of stromal cells and the frequency of mature Lin ϩ cells is extremely low (8,9).…”

Section: Primitive Cell Enrichmentmentioning

confidence: 99%

“…Low-density mononuclear cells (MNCs; Ͻ1.077 g/ml) were isolated by standard methods, using Ficoll-Paque Plus (Pharmacia Biotech, Uppsala, Sweden), as previously described (8,9).…”

Section: Cell Processingmentioning

confidence: 99%

“…Recently, we have characterized a hematopoietic cell population, obtained by negative selection, which consists of lineage-negative (Lin Ϫ ) cells, and is enriched for primitive cells, including those expressing the CD34 antigen (CD34 ϩ cells) and those capable of forming hematopoietic colonies in semisolid cultures (colony-forming cells; CFCs) (8). In keeping with the concept mentioned earlier, we have observed that, when cultured in serumfree liquid cultures in the presence of a rich cytokine cocktail, progenitor cell-enriched Lin Ϫ cell populations derived from UCB show significantly higher in vitro growth capacities than equivalent populations from aMPB (9).…”

Section: Introductionmentioning

confidence: 99%

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Cell Cycle Differences in Vitro between Primitive Hematopoietic Cell Populations from Adult and Umbilical Cord Blood

Alvarado-Moreno

Chávez‐González

Cérbulo

et al. 2007

Stem Cells and Development

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Lineage-negative (Lin(-)) cell populations, obtained by negative selection from umbilical cord blood (UCB) and adult mobilized peripheral blood (aMPB), were cultured in serum-free liquid cultures supplemented with a mixture of seven stimulatory cytokines. On specific days, proliferation potential was assessed and cell cycle status was determined by DNA content. Expression of the cell cycle regulators cyclin D3 (cD3), cyclin-dependent kinase 4 (cdk4), p21(cip1/waf1) (p21), and p27(kip1) (p27) was also determined. As expected, UCB cells showed significantly higher proliferation potentials than aMPB cells, particularly during the first 7 days of culture. During this period of time, higher numbers of cell cycles were observed in UCB cells (7-9 cycles), as compared to aMPB cells (5-6 cycles). Higher levels of cD3, cdk4, and p27 were also detected in UCB cells. Our results confirm that UCB cells possess an intrinsically higher proliferation potential, as compared to aMPB cells, and suggest that such a biological difference is due, at least in part, to differences in cell cycle status. This, in turn, seems to result from the differential expression of cell cycle regulatory molecules.

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Section: Primitive Cell Enrichmentmentioning

confidence: 49%

“…On the indicated days, CD34 ϩ cells were identified and quantified by immunocytochemistry, both before and after negative selection, as previously described (8,9).…”

Section: Cd34 ϩ Cell Quantificationmentioning

confidence: 99%

Section: Primitive Cell Enrichmentmentioning

confidence: 99%

“…Low-density mononuclear cells (MNCs; Ͻ1.077 g/ml) were isolated by standard methods, using Ficoll-Paque Plus (Pharmacia Biotech, Uppsala, Sweden), as previously described (8,9).…”

Section: Cell Processingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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