In vitro characterization of a human neural progenitor cell coexpressing SSEA4 and CD133

Barraud, Perrine; Stott, Simon; Møllgård, Kjeld; Parmar, Malin; Björklund, Anders

doi:10.1002/jnr.21116

Cited by 88 publications

(69 citation statements)

References 43 publications

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“…In this study, the neuroepithelium of 6 th wpc embryos was YKL-40-positive with cytoplasmic staining and demonstrated a gradient with a strong reactivity confined to the midline corresponding to the distribution of a subset of SSEA (stage-specific embryonic antige)4-positive neuroepithelial and early radial glial cells; this was also shown previously in the same material (Barraud et al 2007). Although subtle, the cytoplasmic YKL-40 staining spanned the entire VZ, with processes into the PP and increased reactivity in the outermost part of the PP, suggesting that YKL-40 is expressed by neuroepithelial cells/radial glial cells (neural stem cells) in the early VZ.…”

Section: Neuroepithelial Cells Radial Glial Cells and Astroglial Prosupporting

confidence: 87%

Brain Barriers and a Subpopulation of Astroglial Progenitors of Developing Human Forebrain Are Immunostained for the Glycoprotein YKL-40

Bjørnbak

Brøchner

Larsen

et al. 2014

J Histochem Cytochem.

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SummaryYKL-40, a glycoprotein involved in cell differentiation, has been associated with neurodevelopmental disorders, angiogenesis, neuroinflammation and glioblastomas. We evaluated YKL-40 protein distribution in the early human forebrain using double-labeling immunofluorescence and immunohistochemistry. Immunoreactivity was detected in neuroepithelial cells, radial glial end feet, leptomeningeal cells and choroid plexus epithelial cells. The subpial marginal zone was YKL-40-positive, particularly in the hippocampus, from an early beginning stage in its development. Blood vessels in the intermediate and subventricular zones showed specific YKL-40 reactivity confined to pericytes. Furthermore, a population of YKL-40-positive, small, rounded cells was identified in the ventricular and subventricular zones. Real-time quantitative RT-PCR analysis showed strong YKL-40 mRNA expression in the leptomeninges and the choroid plexuses, and weaker expression in the telencephalic wall. Immunohistochemistry revealed a differential distribution of YKL-40 across the zones of the developing telencephalic wall. We show that YKL-40 is associated with sites of the brain barrier systems and propose that it is involved in controlling local angiogenesis and access of peripheral cells to the forebrain via secretion from leptomeningeal cells, choroid plexus epithelium and pericytes. Furthermore, we suggest that the small, rounded, YKL-40-positive cells represent a subpopulation of astroglial progenitors, and that YKL-40 could be involved in the differentiation of a particular astrocytic lineage. (J Histochem Cytochem 62:369-388, 2014)

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Section: Neuroepithelial Cells Radial Glial Cells and Astroglial Prosupporting

confidence: 87%

Brain Barriers and a Subpopulation of Astroglial Progenitors of Developing Human Forebrain Are Immunostained for the Glycoprotein YKL-40

Bjørnbak

Brøchner

Larsen

et al. 2014

J Histochem Cytochem.

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“…Stage-specific embryonic antigen-4 is commonly used as a cell surface marker to identify the pluripotent human ESC and SSEA-4 expressing cells enriched in the neural stem/progenitor cell fraction. 21 It was strongly expressed in neuronal cell bodies but virtually absent in extending processes (black vs. white arrowhead, Fig. 1K).…”

Section: Resultsmentioning

confidence: 99%

Steroid-mediated differentiation of neural/neuronal cells from epithelial ovarian precursors in vitro

Bukovský¹,

Caudle²,

Svetlikova³

2008

Cell Cycle

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We reported earlier that occasional neurons evolve in human cultures of pluripotent ovarian epithelial stem cells. In subsequent experiments, frequent transdifferentiation into neural stem cells (NSC) and differentiating neurons was observed in human ovarian epithelial stem cells and porcine granulosa cells after exposure to certain combinations of sex steroids. Testosterone (TS), progesterone (PG) or estradiol (E2) alone do not increase the emergence of neurons. However, a mixture of TS + PG after E2 pretreatment converted a majority of ovarian epithelial stem cells or porcine granulosa cells into NSC and differentiating neuronal cells within one to three hours. Cultured neurons manifested an interconnectivity resembling primitive neuronal pathways in culture. These converted cells expressed the cell markers SSEA-1, SSEA-4, NCAM and Thy-1 glycoconjugates of NSC and neurons, and differentiating cells showed characteristic neuronal morphology. Emergence of NSC and neuronal cells was associated with significant cellular depletion of L-glutamic acid (glutamate), which serves as the major excitatory neurotransmitter in the vertebrate CNS and its fast removal is essential for preventing glutamate excitotoxicity. These observations suggest that certain sequential systemic treatment with common sex steroids and their mixture might be effective in the treatment or prevention of degenerative CNS disorders. The ovarian stem cell cultures readily obtainable from human ovaries regardless of the woman's age have the potential to produce NSC for autologous regenerative treatment of neurologic diseases in aging women. Finally, the proper combination of sex steroids could possibly be employed for transdifferentiation of adult bone marrow stem cells or mobilized peripheral blood cells into autologous NSC and stimulate their neuronal differentiation after homing in the CNS.

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“…CD133 was first recognized in CD34 + progenitor populations from adult blood and bone marrow and high levels of CD133 expression is now accepted as a characteristic for hematopoietic and neural stem cells (33)(34)(35). We detected low levels of CD133 in the MeT5A non-malignant human pleural cells.…”

Section: Discussionmentioning

confidence: 99%

Putative cancer stem cells in malignant pleural mesothelioma show resistance to cisplatin and pemetrexed

Schmid¹

2010

Int J Oncol

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Abstract. Malignant pleural mesothelioma (MPM) is a lethal cancer of the mesothelium with high chemotherapeutic resistance via unknown mechanisms. A prevailing hypothesis states that cancer stem cells (CSCs) persist in tumors causing relapse after chemotherapy, thus, rendering these cells as critical targets responsible for tumor resistance and recurrence. We selected candidate CSC markers based on expansion under hypoxic conditions, a hallmark for the selection of chemoresistant cells; and investigated the expression of CSC markers: CD133, Bmi-1, uPAR and ABCG2 in three MPM cell lines and normal mesothelial cells by quantitative RT-PCR. Furthermore, we evaluated the chemotherapeutic resistance associated with each CSC marker by determining the change in CSC marker-mRNA levels as an index of drugresistance following treatment with either cisplatin or pemetrexed. We demonstrate the expression of CSC markers: CD133, Bmi-1, uPAR and ABCG2 in both normal and MPM cell lines. Bmi-1 + , uPAR + and ABCG2 + cells show a distinct role in conferring chemoresistance to cisplatin and pemetrexed in the malignant setting. By contrast, these markers have no apparent participation in chemoresistance to drug treatments in normal mesothelial cells. Intriguingly, CD133 revealed chemoresistant properties in both normal mesothelial and malignant pleural mesothelioma cells. This study provides evidence of putative CSCs conferring drug-resistance to cisplatin and pemetrexed in MPM cell lines. Specific targeting of these drug-resistant cells, while considering the functional heterogeneity of the MPM subtypes, may contribute to more focused and effective chemotherapeutic regimens for malignant pleural mesothelioma.

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In vitro characterization of a human neural progenitor cell coexpressing SSEA4 and CD133

Cited by 88 publications

References 43 publications

Brain Barriers and a Subpopulation of Astroglial Progenitors of Developing Human Forebrain Are Immunostained for the Glycoprotein YKL-40

Brain Barriers and a Subpopulation of Astroglial Progenitors of Developing Human Forebrain Are Immunostained for the Glycoprotein YKL-40

Steroid-mediated differentiation of neural/neuronal cells from epithelial ovarian precursors in vitro

Putative cancer stem cells in malignant pleural mesothelioma show resistance to cisplatin and pemetrexed

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