In Vitro Characterisation Revealed Himalayan Dairy Kluyveromyces marxianus PCH397 as Potential Probiotic with Therapeutic Properties

Nag, Deepika; Goel, Abhishek; Padwad, Yogendra; Singh, Dharam

doi:10.1007/s12602-021-09874-5

Cited by 15 publications

(15 citation statements)

References 54 publications

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“…12 The assessment of the safety of K. marxianus PCH 3 97 isolated from kefir was conducted in vitro and in vivo, and the results demonstrated that short-term oral consumption of K. marxianus was safe and well tolerated in a healthy animal model. 13,14 Surprisingly, K. marxianus A4 and A5 decreased the levels of pro-inflammatory cytokines, such as interleukin-1β † Electronic supplementary information (ESI) available. See DOI: https://doi.org/ 10.1039/d3fo01796f a College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, China.…”

Section: Introductionmentioning

confidence: 99%

“…17 Besides, K. marxianus shows β-galactosidase activities, which are useful for prebiotics synthesis, such as galactooligosaccharides (GOS). 13 We isolated K. marxianus from Tibetan kefir grains (TKGs), which are natural starter cultures containing abundant yeast and bacteria. Our previous study has shown that K. marxianus YG-4 is the dominant fungal taxon in TKGs.…”

Section: Introductionmentioning

confidence: 99%

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Kluyveromyces marxianus supplementation ameliorates alcohol-induced liver injury associated with the modulation of gut microbiota in mice

Cui,

Guo,

Ning

et al. 2023

Food Funct.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Kluyveromyces marxianus supplementation ameliorates alcohol-induced liver injury associated with the modulation of gut microbiota in mice

Cui,

Guo,

Ning

et al. 2023

Food Funct.

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“…The unbound stain was washed and micrographs were captured at 20X magnification by EVOS FL Auto2 microscope (Thermo Fischer Scientific, Waltham, MA, USA). Lipid‐bound ORO was extracted in 100 % isopropanol, and absorbance was measured at 492 nm using a Synergy H1 microplate reader [42] …”

Section: Methodsmentioning

confidence: 99%

“…Preadipocytes were split at 1×10 5 cells per well into 12 well plates and allowed to differentiate with a 25 μM dose of each (1–5) BSAS analogues. Following differentiation, cells were processed from each well to extract total triglycerides by using LPL buffer, and triglyceride (TG) levels were estimated using a commercially available colorimetric Kit (ERBA, Mannheim, Germany), and absorbance was recorded at 505 nm using a Synergy H1 microplate reader [43] …”

Section: Methodsmentioning

confidence: 99%

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Synthesis, Anti‐adipogenic, and Insulin‐sensitizing Potential of Benzosuberene‐alkyl Sulfone (BSAS) Analogues

Goel

Yamini

Gusain

et al. 2023

Chemistry An Asian Journal

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Obesity and diabetes are the leading cause of mortality worldwide. Promising modulators exhibiting insulin sensitivity and anti-adipogenic actions are highly desirable. Organic sulfone analogues are prominent structural units present in diverse bioactive molecules. However, sulfonesderived anti-adipogenic agents are currently unavailable. Therefore, different benzosuberene-alkyl sulfone (BSAS) analogues were synthesized inspired by the molecular diversity of the essential oil of Cedrus deodara. Among the series, five BSAS analogues were assessed in-silico for their binding towards native Peroxisome Proliferator-Activated Receptor Gamma (PPARγ) and further validated in-vitro for their anti-adipogenic potential in differentiated 3T3-L1 pre-adipocytes. Their ability to improve insulin sensitivity was also examined in L6 myotubes to ascertain their role in insulin resistance. Our results demonstrated that BSAS-1 was the partial PPARγ agonist. It has reduced adipogenesis efficiently by downregulating adipogenic genes, resulting in lower triglyceride levels. BSAS-1 lowered oxidative stress, protected myotubes from insulin resistance, and improved glucose uptake by promoting GLUT4 translocation. Overall, BSAS-1 has the potential to treat obesity and obesity-induced insulin resistance.

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Formononetin‐Vitamin E Conjugate Synergistically Supports Adipogenesis, Attenuates Oxidative Stress, and Restores Insulin Sensitization in Differentiated Preadipocytes

Kumar Dhritlahre,

Goel,

Rana

et al. 2024

ChemistrySelect

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In this study, a hybrid conjugate of formononetin (FMN) and vitamin E (VESylated‐FMN) was synthesized through succinic acid linker and investigated for its synergistic effect in diabetes. The conjugate was characterized through Ultra Performance Liquid Chromatography – Mass Spectrometry (UPLC‐MS), High‐Resolution Mass Spectrometry (HRMS), Nuclear Magnetic Resonance (NMR), and Differential Scanning Calorimetry (DSC). The ability of VESylated‐FMN to promote adipogenesis and ameliorate insulin resistance brought on by oxidative stress was evaluated in differentiated preadipocytes. Interestingly, VESylated‐FMN promoted intracellular glucose absorption, which in turn increased lipid and triglyceride accumulation and thereby encouraged adipogenesis. VESylated‐FMN attenuated oxidative stress and promoted cell survival by reducing reactive oxygen species (ROS) generation and reversing apoptosis. Additionally, VESylated‐FMN improved insulin sensitization by upregulating the gene and protein expression of insulin‐sensitizing markers. Overall, FMN in conjugation with vitamin E can effectively augment prior insulin sensitivity by reducing oxidative stress for efficient glycemic control in hyperglycemia.

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In Vitro Characterisation Revealed Himalayan Dairy Kluyveromyces marxianus PCH397 as Potential Probiotic with Therapeutic Properties

Cited by 15 publications

References 54 publications

Kluyveromyces marxianus supplementation ameliorates alcohol-induced liver injury associated with the modulation of gut microbiota in mice

Kluyveromyces marxianus supplementation ameliorates alcohol-induced liver injury associated with the modulation of gut microbiota in mice

Synthesis, Anti‐adipogenic, and Insulin‐sensitizing Potential of Benzosuberene‐alkyl Sulfone (BSAS) Analogues

Formononetin‐Vitamin E Conjugate Synergistically Supports Adipogenesis, Attenuates Oxidative Stress, and Restores Insulin Sensitization in Differentiated Preadipocytes

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