In vitro Cellulose Rich Organic Material Degradation by Cellulolytic Streptomyces albospinus (MTCC 8768)

Abstract

Aims: Cellulosic biomass is the only foreseeable sustainable source of fuels and is also one of the dominating waste materials in nature resulting from human activities. Keeping in view the environmental problems like disposal of large volumes of cellulosic wastes and shortage of fossil fuel in the world, the main aim of the present investigation was to characterize and study the cellulolytic activity of Streptomyces albospinus (MTCC 8768), isolated from municipal wastes, on natural cellulosic substrates viz. straw powder, wood powder and finely grated vegetable peels. Methodology and Result: Stanier's Basal broth with 100 mg of each of the substrates was inoculated separately with S. albospinus (MTCC No. 8768) and incubated at 37 °C for 8 days. The cellulosic substrates were re-weighed at an interval of 2 days and the difference between the initial weight and the final weight gave the amount of substrates degraded by the isolate. It was observed that maximum degradation was observed in the grated vegetable peels (64 mg) followed by straw powder (38 mg) and wood powder (28 mg) over a period of 8 days. Conclusion, significance and impact of study: By the selection of efficient cellulolytic microorganisms and costeffective operational techniques, the production of useful end products from the biodegradation of the low cost enormous stock of cellulose in nature can be very beneficial.

In vitro probiotic profile based selection of indigenous actinobacterial probiont Streptomyces sp. JD9 for enhanced broiler production

“…Biochemical tests were used to differentiate even closely related organisms based on the identification of various metabolic properties of different bacterial species. In this work, the isolate JD9 showed positive and negative reactions for various biochemical tests and they were partially in accordance with the reports of Prasad et al (44) and Rao et al (45).…”

Lignocellulose-Adapted Endo-Cellulase Producing Streptomyces Strains for Bioconversion of Cellulose-Based Materials

“…Some studies have been carried out in order to test the potential of cellulases from Streptomyces strains in the saccharification of different lignocellulose biomasses, such as rice straw (Ball and McCarthy, 1988) and cellulose rich organic materials (Prasad et al, 2012). In the last few years, for sustainable production of biofuels and chemicals, the research interests are focused toward the utilization of crops, such as A. donax (common name giant reed), that are not employed for nutritional uses and completely match the principal objectives of the second generation biofuels (Corno et al, 2014).…”

Fatty acids and survival of bacteria in Hammam Pharaon springs, Egypt

“…The amylolytic, cellulolytic, lipolytic and proteolytic activities of the three isolates were qualitatively assayed according to [15], [16] and [17], respectively. The a-amylase activity was tested by starch hydrolysis was monitored by Iodine reagent [15].…”