In Vitro Cellular Immune Responses to Recombinant Antigens of<i>Mycobacterium avium</i>subsp.<i>paratuberculosis</i>

Shin, Sung Jae; Chang, Chin Sung; Chang, Chao-Pei Betty; McDonough, Sean P.; Thompson, Belinda; Yoo, Han Sang; Chang, Yung Fu

doi:10.1128/iai.73.8.5074-5085.2005

Cited by 40 publications

(22 citation statements)

References 52 publications

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“…Among the vaccine antigens used in this study, secreted proteins MAP0217 and MAP1609c (Ag85B) are expected to be highly immunogenic due to their extracellular environment and high likelihood of encountering immune cells (35,36). However, we found that postvaccination immune responses of the animals with these antigens were weak compared to responses with the other recombinant proteins.…”

Section: Discussioncontrasting

confidence: 49%

Cell-Mediated and Humoral Immune Responses after Immunization of Calves with a Recombinant Multiantigenic Mycobacterium avium subsp. paratuberculosis Subunit Vaccine at Different Ages

Thakur

Aagaard

Stockmarr

et al. 2013

Clin Vaccine Immunol

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cNeonates and juvenile ruminants are very susceptible to paratuberculosis infection. This is likely due to a high degree of exposure from their dams and an immature immune system. To test the influence of age on vaccine-induced responses, a cocktail of recombinant Mycobacterium avium subsp. paratuberculosis proteins (MAP0217, MAP1508, MAP3701c, MAP3783, and MAP1609c/Ag85B) was formulated in a cationic liposome adjuvant (CAF01) and used to vaccinate animals of different ages. Male jersey calves were divided into three groups that were vaccinated at 2, 8, or 16 weeks of age and boosted twice at weeks 4 and 12 relative to the first vaccination. Vaccine-induced immune responses, the gamma interferon (IFN-␥) cytokine secretion and antibody responses, were followed for 20 weeks. In general, the specific responses were significantly elevated in all three vaccination groups after the first booster vaccination with no or only a minor effect from the second booster. However, significant differences were observed in the immunogenicity levels of the different proteins, and it appears that the older age group produced a more consistent IFN-␥ response. In contrast, the humoral immune response is seemingly independent of vaccination age as we found no difference in the IgG1 responses when we compared the three vaccination groups. Combined, our results suggest that an appropriate age of vaccination should be considered in vaccination protocols and that there is a possible interference of vaccine-induced immune responses with weaning (week 8).

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Section: Discussioncontrasting

confidence: 49%

Cell-Mediated and Humoral Immune Responses after Immunization of Calves with a Recombinant Multiantigenic Mycobacterium avium subsp. paratuberculosis Subunit Vaccine at Different Ages

Thakur

Aagaard

Stockmarr

et al. 2013

Clin Vaccine Immunol

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“…The findings suggest that not all M. avium subsp. paratuberculosis proteins known to be differentially regulated under stress conditions are of potential diagnostic value, as found by other studies (33,34). There is a need to investigate other M. avium subsp.…”

Section: Discussionmentioning

confidence: 90%

Lymphoproliferative and Gamma Interferon Responses to Stress-Regulated Mycobacterium avium subsp. paratuberculosis Recombinant Proteins

Gurung

Begg

Purdie

et al. 2014

Clin Vaccine Immunol

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bJohne's disease in ruminants is a chronic infection of the intestines caused by Mycobacterium avium subsp. paratuberculosis. An important strategy to control disease is early detection, and a potentially efficient method for early detection is measurement of cell-mediated immune responses developed by the host in response to exposure or infection. One method is to measure lymphoproliferation and cytokine release from the host cells when exposed to the organism or parts of the organism. In this study, 10 recombinant M. avium subsp. paratuberculosis proteins known to be upregulated under in vitro stress conditions were evaluated by examining their ability to evoke memory as a result of exposure by vaccination or oral challenge with live Mycobacterium avium subsp. paratuberculosis. Out of 10 proteins, MAP2698c was found to induce higher cell-mediated immune responses in vaccinated and challenged sheep in comparison to healthy controls. The findings suggest that not all stress-regulated proteins have the diagnostic potential to detect cell-mediated immune responses in ovine paratuberculosis.

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“…One of the antigens recognized by M. avium subsp. paratuberculosis infected cattle is a 35-kDa major membrane protein (MMP) (33,34) that most likely corre- sponds to the protein detected with sera from two of the calves in the current study (Fig. 2).…”

Section: Discussionmentioning

confidence: 99%

Divergent Immune Responses to Mycobacterium avium subsp. paratuberculosis Infection Correlate with Kinome Responses at the Site of Intestinal Infection

et al. 2013

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In Vitro Cellular Immune Responses to Recombinant Antigens ofMycobacterium aviumsubsp.paratuberculosis

Cited by 40 publications

References 52 publications

Cell-Mediated and Humoral Immune Responses after Immunization of Calves with a Recombinant Multiantigenic Mycobacterium avium subsp. paratuberculosis Subunit Vaccine at Different Ages

Cell-Mediated and Humoral Immune Responses after Immunization of Calves with a Recombinant Multiantigenic Mycobacterium avium subsp. paratuberculosis Subunit Vaccine at Different Ages

Lymphoproliferative and Gamma Interferon Responses to Stress-Regulated Mycobacterium avium subsp. paratuberculosis Recombinant Proteins

Divergent Immune Responses to Mycobacterium avium subsp. paratuberculosis Infection Correlate with Kinome Responses at the Site of Intestinal Infection

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