In vitro cell compatibility study of rose bengal–chitosan adhesives

Barton, Matthew; Piller, Sabine C.; Mahns, David A.; Morley, John W.; Mawad, Damia; Longo, Leonardo; Lauto, Antonio

doi:10.1002/lsm.22076

Cited by 23 publications

(20 citation statements)

References 30 publications

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“…It may be speculated that once the RB is photoactivated, and exposed to tissue it may produce toxic reactive oxygen species. Our results however demonstrated that RB toxicity and phototoxicity was not significant, in agreement with previous reports [20,23]. Further still, RB has had a proven clinical history of systemic use for ophthalmic pathological medical diagnosis [34].…”

Section: Discussionsupporting

confidence: 92%

“…Suturing can also be a challenging task especially during minimally invasive procedures. Our group has recently demonstrated that chitosan films, containing RB, can be photochemically bonded to tissue without inducing significant cell toxicity [23].…”

Section: Discussionmentioning

confidence: 99%

“…Preliminary in vitro studies from our laboratory have already shown that RB can be incorporated in a thin chitosan film, which bonded strongly to calf small intestine. During the laser irradiation, the tissue temperature did not exceeded 38 C and no significant cytotoxic effect was observed [22,23]. Therefore, in this study we investigated for the first time the bonding strength, structural integrity (histopathology) and electrophysiogical performance of rat nerves, which were repaired in vivo using our novel RB-chitosan adhesive and compared to nerves repaired by the standard end-to-end suture repair technique.…”

Section: Journal Of Biophotonicsmentioning

confidence: 99%

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Laser‐activated adhesive films for sutureless median nerve anastomosis

Barton

Morley

Stoodley

et al. 2013

Journal of Biophotonics

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A novel chitosan adhesive film that incorporates the dye 'Rose Bengal' (RB) was used in conjunction with a green laser to repair transected rat median nerves in vivo. Histology and electrophysiological recording assessed the impact of the laser-adhesive technique on nerves. One week post-operatively, the sham-control group (laser-adhesive technique applied on un-transected nerves) conserved the average number and size of myelinated fibres in comparison to its contralateral side and electrophysiological recordings demonstrated no significant difference with un-operated nerves. Twelve weeks after the laser-adhesive anastomoses, nerves were in continuity with regenerated axons that crossed the anastomotic site.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Journal Of Biophotonicsmentioning

confidence: 99%

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Laser‐activated adhesive films for sutureless median nerve anastomosis

Barton

Morley

Stoodley

et al. 2013

Journal of Biophotonics

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“…Moreover, photo-activated RB has been revealed to be nontoxic to cells in vitro and in vivo [2,3]. When RB was incorporated into a chitosan bandage for median nerve repair, the bandage immediately and effectively sealed the epineurium, minimised inflammation and scar tissue infiltration, thereby providing the necessary mechanical support to the nerve while the underlying fibroblasts were able to regenerate and close the epineurium [2].…”

Section: Laser-activated Dyesmentioning

confidence: 99%

Nerve repair: toward a sutureless approach

et al. 2014

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Peripheral nerve repair for complete section injuries employ reconstructive techniques that invariably require sutures in their application. Sutures are unable to seal the nerve, thus incapable of preventing leakage of important intraneural fluids from the regenerating nerve. Furthermore, sutures are technically demanding to apply for direct repairs and often induce detrimental scarring that impedes healing and functional recovery. To overcome these limitations, biocompatible and biodegradable glues have been used to seal and repair peripheral nerves. Although creating a sufficient seal, they can lack flexibility and present infection risks or cytotoxicity. Other adhesive biomaterials have recently emerged into practice that are usually based on proteins such as albumin and collagen or polysaccharides like chitosan. These adhesives form their union to nerve tissue by either photothermal (tissue welding) or photochemical (tissue bonding) activation with laser light. These biomaterial adhesives offer significant advantages over sutures, such as their capacity to unite and seal the epineurium, ease of application, reduced invasiveness and add the potential for drug delivery in situ to facilitate regeneration. This paper reviews a number of different peripheral nerve repair (or reconstructive) techniques currently used clinically and in experimental procedures for nerve injuries with or without tissue deficit.

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“…This promotes a significant increase in the binding of the chitosan to the nerve segments, providing a sutureless anastomosis. 16,17 Chitosan with rose bengal appears to be biologically safe 18,19 and provides tensile strength that is similar to microsuture repair. Barton et al 17 compared the strength of microsuture and chitosan repair in a rat median nerve model and found no difference in the acute binding strength (0.42 6 0.08 N vs. 0.37 6 0.15 N), although there was a difference 1 week postoperatively (0.54 6 0.14 N vs. 0.36 6 0.11 N, respectively; P < .008).…”

Section: Introductionmentioning

confidence: 99%