In vitro cartilage tissue engineering with 3D porous aqueous-derived silk scaffolds and mesenchymal stem cells

Wang, Yongzhong; Kim, Ung-Jin; Blasioli, Dominick J.; Kim, Hyeon-Joo; Kaplan, David L.

doi:10.1016/j.biomaterials.2005.05.022

Cited by 405 publications

(296 citation statements)

References 65 publications

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“…On the contrary, collagen is not only a natural molecule but also the main component of the extracellular matrix of IVD. Also natural materials, when not prepared properly, might be unsuitable as supports for in vivo transplantation because of fast degradation rates, possible transmission of pathogens and viruses from the derivation source of the material, and undesired breakdown products [29]. We might be able to overcome some of these problems by using bio-materials already approved for human use, such as wound healing or in surgery, which will have the advantages of being supplied sterile, validated to be non- toxic and biocompatible, thus simplifying lengthy and expensive procedures for translation of research into clinic.…”

Section: Discussionmentioning

confidence: 99%

“…To induce MSC chondrogenesis growth factors such as transforming growth factor (TGF)-b1, 2 or 3 [18], bone morphogenetic protein-2 [19], -4 [20], -7 [21] or -14 (GDF-5) [22], have been described to facilitate MSCs differentiation, however the aim of this project was to focus on the differences caused by variety of matrixes. In particular, many studies already described culture of MSCs embedded in several types of scaffolds, such as agarose gels [23], alginate beads [24][25][26], synthetic polymers [27,28] and other biomaterials [29][30][31][32]. These studies provide clear evidence that there is a need of a cell support for enhancing cell-cell and cell-matrix interactions and creating a 3D environment.…”

Section: Introductionmentioning

confidence: 99%

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Influence of different commercial scaffolds on the in vitro differentiation of human mesenchymal stem cells to nucleus pulposus-like cells

et al. 2011

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Introduction Cell-based therapies for regeneration of the degenerated intervertebral disc (IVD) are an alternative to current surgical intervention. Mesenchymal stem cells (MSCs), in combination with a scaffold, might be ideal candidates for regenerating nucleus pulposus (NP), the pressure-distributing part of the IVD. While the use of growth factors for MSCs differentiation currently receives major attention, in this study we compare the performance of sponge-like matrixes in supporting cell differentiation into NP-like cells. Materials and methods Four types matrixes approved as medical devices for other applications were tested as scaffolds for MSCs: two made of equine or porcine collagen, one of gelatin and one of chitosan. Bone marrowderived human MSCs were seeded in these scaffolds or embedded in alginate, as a three-dimensional control. After five weeks in culture, NP-like differentiation of the cellscaffold constructs was analyzed by qRT-PCR, histology, total DNA quantification, proteoglycan accumulation and immunohistochemistry. Results MSCs in collagen matrixes and gelatin produced more mRNA and proteins of the chondrogenic markers collagen type I, collagen type II (COL2) and aggrecan (ACAN), when compared with cells embedded in alginate or chitosan. Proteoglycan accumulation and cell survival were also higher in collagen and gelatin matrixes. Gene expression results were also confirmed by histological and immunohistochemical staining. In contrast to alginate control, the gene expression of the undesired bone marker osteopontin was lower in all tested groups. In porcine collagen supports, MSC expression ratio between COL2/ ACAN closely resembled the expression of nucleus pulposus cells, but gene expression of recently described NP markers keratin19, PAX1 and FOXF1 was lower. Conclusions Collagen supports provide a readily available, medically approved and effective scaffold for chondrogenic differentiation in vitro, but the phenotype of differentiated MSCs is not yet completely equivalent to that of NP cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influence of different commercial scaffolds on the in vitro differentiation of human mesenchymal stem cells to nucleus pulposus-like cells

et al. 2011

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“…Hypoxia inducible factor (HIF) mediates transcription factors to allow chondrocytes to adapt to low oxygen tension [120]. Hypoxia has been shown to increase the synthesis of ECM proteins in vitro in both chondrocytes as well as hypoxia-induced chondrogenic differentiation of MSCs [67,121,122]. Hypoxia has also been shown to inhibit the expression of collagen Type X, present in fibrocartilage and a marker of chondrocyte hypertrophy [123,124].…”

Section: Oxygen Tensionmentioning

confidence: 99%

Bioengineering of articular cartilage: past, present and future

Felimban

Moulton

et al. 2013

Regenerative Medicine

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The treatment of cartilage defects poses a clinical challenge owing to the lack of intrinsic regenerative capacity of cartilage. The use of tissue engineering techniques to bioengineer articular cartilage is promising and may hold the key to the successful regeneration of cartilage tissue. Natural and synthetic biomaterials have been used to recreate the microarchitecture of articular cartilage through multilayered biomimetic scaffolds. Acellular scaffolds preserve the microarchitecture of articular cartilage through a process of decellularization of biological tissue. Although promising, this technique often results in poor biomechanical strength of the graft. However, biomechanical strength could be improved if biomaterials could be incorporated back into the decellularized tissue to overcome this limitation.

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“…92 A second class of scaffolds is naturally occurring organic materials that provide a bio-mimetic environment for stem cells. Human BMSCs regenerate bone in marine sponge skeletons, 93 cartilage in silk fibroin scaffolds, 94 and adipose tissue in gelatin. 95 In addition to providing mechanical strength, natural scaffolds can contain biological agents that influence stem cell fate; marine sponge skeletons, for example, contain the cell adhesion proteins fibronectin and tenascin.…”

Section: Scaffoldsmentioning

confidence: 99%

Review:Ex VivoEngineering of Living Tissues with Adult Stem Cells

et al. 2006

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Adult stem cells have the potential to revolutionize regenerative medicine with their unique abilities to self-renew and differentiate into various phenotypes. This review examines progress and challenges in ex vivo tissue engineering with adult stem cells. These rare cells are harvested from a variety of tissues, including bone marrow, adipose, skeletal muscle, and placenta, and differentiate into cells of their own lineage and in some cases atypical lineages. Insight into the stem cell niche leads to the identification of matrix components, soluble factors, and physiological conditions that enhance the ex vivo amplification and differentiation of stem cells. Scaffolds composed of metals, naturally occurring materials, and synthetic polymers organize stem cells into complex spatial groupings that mimic native tissue. Cell signals from covalently bound ligands and slowly released regulatory factors in scaffolds direct stem cell fate. Future advances in stem cell biology and scaffold design will ultimately improve the efficacy of tissue substitutes as implants, in research, and as extracorporeal devices.

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In vitro cartilage tissue engineering with 3D porous aqueous-derived silk scaffolds and mesenchymal stem cells

Cited by 405 publications

References 65 publications

Influence of different commercial scaffolds on the in vitro differentiation of human mesenchymal stem cells to nucleus pulposus-like cells

Influence of different commercial scaffolds on the in vitro differentiation of human mesenchymal stem cells to nucleus pulposus-like cells

Bioengineering of articular cartilage: past, present and future

Review:Ex VivoEngineering of Living Tissues with Adult Stem Cells

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