In vitro cardiomyogenic potential of human umbilical vein-derived mesenchymal stem cells

Kadivar, Mehdi; Khatami, Shohreh; Mortazavi, Yousef; Shokrgozar, Mohammad Ali; Taghikhani, Mohammad; Soleimani, Masoud

doi:10.1016/j.bbrc.2005.12.047

Cited by 131 publications

(104 citation statements)

References 26 publications

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“…It has been demonstrated that, in some culture conditions, progenitor cells are able to transdifferentiate into vascular cells or cardiomyocytes [18,19]. We isolated and cultured MSC and EPC that presented the same characteristics and properties as previously reported [20,21].…”

Section: Progenitor Cell Differentiationmentioning

confidence: 92%

Epicardial deposition of endothelial progenitor and mesenchymal stem cells in a coated muscle patch after myocardial infarction in a murine model☆

Derval

Barandon

Dufourcq

et al. 2008

European Journal of Cardio-Thoracic Surgery

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Objectives: To assess, using an in vivo engraftment strategy combining bone marrow cell (BMC) transplantation and tissue cardiomyoplasty, the functional outcome of distinct vascular progenitor cell therapy (endothelial progenitor (EPC) and mesenchymal stem (MSC) cells) at distance of myocardium infarction (MI). The study was also designed to test whether scaffold mixing progenitors with unfractionated BMC could improve progenitor recruitment in the damaged myocardium. Methods: To track engrafted progenitor cells in vivo, cultured murine MSC and EPC were transduced with eGFP lentiviruses. Thirty days after cryogenical induction of MI, C57BL/6J mice were randomized to receive muscle patch placement coated or not (control group), labeled EPC or MSC mixed to the ration of 1:10, or not with unfractionated BMC. Two weeks after transplantation, cardiac function was recorded and heart sections were examined to detect GFP-labeled progenitor cells and analyze cell differentiation. Results: This study showed that either type of mono cell therapy improved angiogenesis and cell survival in the scar but only MSC exhibited the capacity to invade the scar. We found no evidence of myocardial or vascular regeneration from progenitor cells. Engraftment of the progenitors/unfractionated BMC mix increased repopulation and thickness of the scar. Conclusion: Combined therapy with unfractionated BMC and expanded MSC appeared thus promising for scar repopulation. #

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Section: Progenitor Cell Differentiationmentioning

confidence: 92%

Epicardial deposition of endothelial progenitor and mesenchymal stem cells in a coated muscle patch after myocardial infarction in a murine model☆

Derval

Barandon

Dufourcq

et al. 2008

European Journal of Cardio-Thoracic Surgery

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“…These stem cells are capable of differentiating into various cellular lineages, including the osteogenic, chondrogenic, adipogenic, and myogenic lineages (Pittenger et al, 1999;Jiang et al, 2002;Imabayashi et al, 2003;Bosch et al, 2006), and are widely used in clinical investigations (Friedenstein et al, 1966;Horwitz et al, 2002;Kadivar et al, 2006;Yang et al, 2008). Before human clinical trials can be approved, scaled-up cell production and delivery into an animal model with cell doses (number of cells) comparable to those anticipated for human trials are often required in order to satisfy regulatory safety concerns.…”

Section: Introductionmentioning

confidence: 99%

Oct4 and Sox2 overexpression improves the proliferation and differentiation of bone mesenchymal stem cells in Xiaomeishan porcine

Fan¹,

Gu²,

Zhang³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Mesenchymal stem cells derived from bone marrow (BMSCs) are a population of self-renewing multipotent cells that are capable of differentiating into various cellular lineages, and are widely employed in tissue engineering and cell therapy. Recently, clinical research involving BMSCs has become increasingly popular. In order to conduct appropriate research, it is first necessary to amplify large amounts of functional BMSCs in vitro. However, after several passages of expanding in vitro, the proliferation and differentiation potential of BMSCs gradually decline. To determine whether overexpression of Oct4 or Sox2 might prevent this decline, we transfected Oct4 or Sox2, which are essential for the pluripotency and self-renewal of embryonic stem cells, into BMSCs of Xiaomeishan porcine by a lentivirus. The results showed that overexpression of Sox2 or Oct4 BMSCs in culture media containing a basic fibroblast growth factor resulted in higher proliferation and differentiation compared to controls, suggesting that genetic modification of stemness-related genes is an efficient way to maintain the proliferation and differentiation potential of BMSCs.

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“…Similarly, in other studies there wasn't' any evidence for neocardiomyocyte formation after systemic delivery of CB-mononuclear cells, or direct intramyocardial delivery of CB-133+ cardiac membrane cells, suggesting that there is a limitation in the potential for differentiation of unmodified CB-derived stem cells into cardiomyocytes (Murry et al, 2004). However, MSCs isolated from endothelial/subendothelial layers of the human umbilical cord veins have been proved to have the potential of transdifferentiating into cardiomyocyte-like cells with typical ultrastructure and sarcomers as well as expression of several cardiac-specific genes (Kadivar et al, 2006). Further studies are warranted to reduce the discrepancy between these research papers.…”

Section: Differential Potential Into Cardiomyocytesmentioning

confidence: 99%

Human Cord Blood-Derived Stem Cells in Transplantation and Regenerative Medicine

Iancu¹,

Ilie²,

Mocan³

et al. 2011

Stem Cells in Clinic and Research

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In vitro cardiomyogenic potential of human umbilical vein-derived mesenchymal stem cells

Cited by 131 publications

References 26 publications

Epicardial deposition of endothelial progenitor and mesenchymal stem cells in a coated muscle patch after myocardial infarction in a murine model☆

Epicardial deposition of endothelial progenitor and mesenchymal stem cells in a coated muscle patch after myocardial infarction in a murine model☆

Oct4 and Sox2 overexpression improves the proliferation and differentiation of bone mesenchymal stem cells in Xiaomeishan porcine

Human Cord Blood-Derived Stem Cells in Transplantation and Regenerative Medicine

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