In vitro callus and shoot regeneration in Enterolobium cyclocarpum (Jacq.) Grised. - a fast timber yielding species

Abstract

This study was conducted to investigate the in vitro callus induction and rapid shoot regeneration potential in Enterolobium cyclocarpum, a plant native to central Mexico but widely introduced into Africa. The leaf, stem and nodal explants of E. cyclocarpum were cultured on full strength Murashige and Skoog (MS) medium supplemented with different concentrations of Cytokinins - Benzyladenine (BA) and/or Kinetin and Auxins - Naphthalene acetic acid (NAA) and/or 2,4-Dichlorophenoxylacetic acid (2,4-D) each alone and in combination. The leaf explants did not respond to these treatments. The Nodal explants were best for caulogenesis, while the explant responses were in the order- nodal > stem > cotyledon for callogenesis in MS medium supplemented with BA and/or Kin combined with NAA and/or 2,4-D. The varied combinations induced white compact callus. The highest callus production was observed on MS medium supplemented with 2.7 µM NAA + 2.2 µM BA and 5.4 µM NAA alone. Nodal and cotyledon explants developed callus and multiple shoots on MS supplemented with a combination of cytokinin (BA and/or Kin.) and auxin (NAA and/or 2,4-D). The maximum number of 3.98 ± 0.37 and 2.1±0.11 shoots/explants were recorded for nodal and cotyledon explants on MS medium supplemented with a combination of 8.8 µM BA+2.7 µM NAA and 2.2µM BA+2.7 µM NAA respectively. On the basal medium, 10% of the excised shoots rooted successfully. Thus, this in vitro method can be exploited for conservation and mass propagation of this fast timber yielding tree and also utilized for embryogenesis studies.

Cultivo in vitro de Peltophorum dubium (Spreng.) Taub y Enterolobium contortisiliquum (Vell.) Morong

“…Como mencionamos más arriba, las publicaciones relacionadas a la multiplicación vía axilar, organogénesis y/o embriogénesis somática en E. contortisiliquum, es inexistente, con excepción al trabajo publicado por Rodrigues et al (2022), quienes describen el cultivo de cotiledones de E. contortisiliquum en medio MS suplementado con 0,5 mg/l 2,4-D + 2,0 mg/l picloram + 0,5 mg/l Kn + 2,0 mg/l BAP, para estudiar la curva de crecimiento in vitro de la especie, reportando solamente la formación de callos y su evolución. Mientras que en E. cyclocarpum, Akinropo et al (2020), observaron que los segmentos nodales cultivados en un medio MS suplementado con 0,5 mg/l de BAP y ANA, generaron la mayor frecuencia de callos. Cuando cultivaron segmentos cotiledonares y nodales en 2 mg/l de BAP combinados con 0,5 mg/l de ANA, produjeron 3,98 brotes adventicios/explantos y el 10% brotes de estos brotes fueron enraizados.…”

“…En el caso particular del género Peltophorum, podemos mencionar el trabajo publicado por Uddin et al (2005) y Bassan et al (2006), quienes demostraron la capacidad de producción de brotes in vitro de P. pterocarpum y P. dubium partiendo de segmentos nodales, brotes apicales y cotiledones de plántulas germinadas in vitro. En el género Enterolobium existen escasos reportes de multiplicación in vitro; pudiéndose mencionar los estudios desarrollado en Enterolobium cyclocarpum a partir de segmentos nodales de plántulas germinadas in vitro (Sahagún et al, 2007;Thirunavoukkarasu et al, 2007;Akinropo et al, 2020).…”

Trypsin inhibitor in Enterolobium contortisiliquum calli grown in the presence of plant growth regulators

“…In general, auxins and cytokinins are the most used regulators for the majority of plants, acting in the development and accumulation pathways of bioactive molecules (Chaâbani et al, 2015). Akinropo et al (2020) used these PGRs in experiments with plants of the Enterolobium genus, and Shim et al (2010) found that the callus culture is currently an important bioprospecting strategy for natural products, which has been used as an alternative in the production of several secondary metabolites.…”