2008
DOI: 10.1091/mbc.e08-03-0239
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In Vitro Budding of Intralumenal Vesicles into Late Endosomes Is Regulated by Alix and Tsg101

Abstract: Endosomes along the degradation pathway leading to lysosomes accumulate membranes in their lumen and thus exhibit a characteristic multivesicular appearance. These lumenal membranes typically incorporate down-regulated EGF receptor destined for degradation, but the mechanisms that control their formation remain poorly characterized. Here, we describe a novel quantitative biochemical assay that reconstitutes the formation of lumenal vesicles within late endosomes in vitro. Vesicle budding into the endosome lume… Show more

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Cited by 91 publications
(93 citation statements)
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References 81 publications
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“…Consistent with this possibility, the convex face of the Bro1 domain of a yeast homolog of ALIX possesses a highly basic patch that could interact with anionic phospholipids (26), and this basic patch is to some extent conserved in ALIX (16). Furthermore, ALIX binds to bilayers that contain the unconventional phospholipid lysobisphosphatidic acid, which is thought to regulate the formation of invaginations with negative curvature at the limiting membrane of late endosomes (15,30).…”
Section: Discussionmentioning
confidence: 92%
“…Consistent with this possibility, the convex face of the Bro1 domain of a yeast homolog of ALIX possesses a highly basic patch that could interact with anionic phospholipids (26), and this basic patch is to some extent conserved in ALIX (16). Furthermore, ALIX binds to bilayers that contain the unconventional phospholipid lysobisphosphatidic acid, which is thought to regulate the formation of invaginations with negative curvature at the limiting membrane of late endosomes (15,30).…”
Section: Discussionmentioning
confidence: 92%
“…This phospholipid has been demonstrated to regulate ILV formation and endosome size (Ikonomov et al, 2003(Ikonomov et al, , 2015. In addition, ILV formation is also regulated by the ESCRT complexes, and the ESCRT-0 factor (Hrs) is a PI(3)P and Rab5 effector (Bache et al, 2003;Falguieres et al, 2008;Lindmo and Stenmark, 2006). PIKfyve and the ESCRTs might cooperate to regulate ILV formation, as the ESCRT-III subunit Vps24 interacts with PI(3,5)P 2 (Lindmo and Stenmark, 2006).…”
Section: ) In Vps34mentioning
confidence: 99%
“…[ALG-2 (apoptosis-linked gene 2) interacting protein X], which inhibits formation of intralumenal vesicles (10,46). Knockdown of all candidates was nearly complete at protein level (Fig.…”
Section: The Architecture Of the Egf-dependent Transcriptional Responmentioning
confidence: 99%
“…ESCRTs couple receptor sorting with the membrane deformation and fission process (7,9) during the formation of intralumenal vesicles containing the EGFR (10). Conversely, EGF itself regulates the pathway, because addition of EGF increases the number of multivesicular endosomes that form in an ESCRT-dependent manner (11,12).…”
Section: Introductionmentioning
confidence: 99%