In vitro biofilm growth on modern voice prostheses

Leonhard, Matthias; Zatorska, Beata; Tan, Yulong; Moser, Doris; Schneider‐Stickler, Berit

doi:10.1002/hed.25053

Cited by 10 publications

(6 citation statements)

References 34 publications

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“…As reported in [ 16 , 35 ], the esophageal surfaces of the investigated prosthesis–the esophageal flange, the valve flap, and the valve seating–were mostly covered by biofilm infestation. There were similar structures, like stiff and compact bulges in the biofilm layers, on the flanges described in previous studies [ 36 , 37 , 38 , 39 ] as we observed in our setting.…”

Section: Discussionmentioning

confidence: 63%

Biofilm Growth Causes Damage to Silicone Voice Prostheses in Patients after Surgical Treatment of Locally Advanced Laryngeal Cancer

et al. 2020

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Voice prosthesis implantation with the creation of a tracheoesophageal fistula is the gold standard procedure for voice rehabilitation in patients after a total laryngectomy. All patients implanted with a voice prosthesis (VP) have biofilms of fungi and bacteria grow on their surface. Biofilm colonization is one of the main reasons for VP degradation that can lead to VP dysfunction, which increases the high risk of pneumonia. In a 20-month evaluation period, 129 cases of prostheses after replacement procedures were investigated. Microbiological examination of the biofilms revealed that there were four of the most common fungi species (Candida spp.) and a large variety of bacterial species present. We studied the relationship between the time of proper function of Provox VP, the microorganism composition of the biofilm present on it, and the degradation level of the silicone material. Evaluation of the surface of the removed VP using an atomic force microscope (AFM) has demonstrated that biofilm growth might drastically change the silicone’s mechanical properties. Changes in silicone stiffness and thermal properties might contribute to the failure of VP function. Our data can serve in future studies for the development of methods to prevent or inhibit biofilm formation on the VP surface that would translate to an increase in their durability and safety.

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Section: Discussionmentioning

confidence: 63%

Biofilm Growth Causes Damage to Silicone Voice Prostheses in Patients after Surgical Treatment of Locally Advanced Laryngeal Cancer

et al. 2020

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“…Four different culture media commonly used in yeast biofilm studies (Lackey et al, 2013;Pereira et al, 2015;Tan et al, 2016;Tan et al, 2017;Leonhard et al, 2018;Goḿez-Molero et al, 2021) were selected and ranked in terms of nutritional content, from highest to lowest: 1) YPD, a complex medium that supports yeast growth without selective conditions (Tan et al, 2016). It contains yeast extract and peptone that stimulate fungal replication.…”

Section: Bacterial Strains and Growth Conditionsmentioning

confidence: 99%

Culture media influences Candida parapsilosis growth, susceptibility, and virulence

Arévalo-Jaimes,

Admella,

Blanco-Cabra

et al. 2023

Front. Cell. Infect. Microbiol.

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IntroductionCandida parapsilosis, a pathogenic yeast associated with systemic infections, exhibits metabolic adaptability in response to nutrient availability.MethodsWe investigated the impact of RPMI glucose supplemented (RPMId), TSB, BHI and YPD media on C. parapsilosis growth, morphology, susceptibility (caspofungin and amphotericin B), and in vivo virulence (Galleria mellonella) in planktonic and biofilm states.ResultsHigh-glucose media favors growth but hinders metabolic activity and filamentation. Media promoting carbohydrate production reduces biofilm susceptibility. Virulence differences between planktonic cells and biofilm suspensions from the same media shows that biofilm-related factors influence infection outcome depending on nutrient availability. Pseudohyphal growth occurred in biofilms under low oxygen and shear stress, but its presence is not exclusively correlated with virulence.DiscussionThis study provides valuable insights into the intricate interplay between nutrient availability and C. parapsilosis pathogenicity. It emphasizes the importance of considering pathogen behavior in diverse conditions when designing research protocols and therapeutic strategies.

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“…An in vitro study on biofilm growth was conducted on various choices of the modern TEP voice prostheses, which concluded that use of silver oxide coating and Teflon as valve flap materials showed significantly less surface biofilm formation. 17 It might more effectively protect users against the current pandemic, since viral particles could penetrate the exopolymeric matrix of mucoid biofilms and thus benefit from a protective environment against external aggressions. 18 A dedicated brush should be used during cleansing of an indwelling device while facing a mirror to prevent colonization of pathogens with drinking water.…”

Section: Voice Rehabilitation Devicesmentioning

confidence: 99%

“…TEP voice prostheses could be indwelling or nonindwelling and made with different materials. An in vitro study on biofilm growth was conducted on various choices of the modern TEP voice prostheses, which concluded that use of silver oxide coating and Teflon as valve flap materials showed significantly less surface biofilm formation 17 . It might more effectively protect users against the current pandemic, since viral particles could penetrate the exopolymeric matrix of mucoid biofilms and thus benefit from a protective environment against external aggressions 18 .…”

Section: Protocol For Care Of Laryngectomy Stoma In Covid‐19mentioning

confidence: 99%

Care of Patients With a Laryngectomy During the COVID‐19 Pandemic

Yeung

Lai

Wong

et al. 2020

Otolaryngol.--head neck surg.

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Patients with a laryngectomy are at increased risk for droplet-transmitted diseases and, therefore, COVID-19, which has now caused a worldwide pandemic. Adaptive measures to protect patients with a laryngectomy and their families were designed and implemented in the Hong Kong SAR (HK). Driven by the fear of severe acute respiratory syndrome in 2003, hospitals in HK have since modified infection control routines to prevent a repeat public health nightmare. To face COVID-19, caused by SARS-CoV-2, we have adapted guidelines for our patients with a laryngectomy. Contact precautions, droplet precautions with physical barriers, and hand and equipment hygiene are our mainstays of prevention against COVID-19, and sharing these routines is the aim of this article. The COVID-19 pandemic is still roaring ahead. Awareness and precautions for patients with a laryngectomy who may be at higher risk are outlined here and should be maintained during the current pandemic.

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In vitro biofilm growth on modern voice prostheses

Abstract: The use of silver oxide and Teflon as valve flap materials proves to reduce long-term biofilm formation in vitro. The applied model allows rapid screening for novel biofilm-inhibitive materials and durable coatings designated for more biofilm resistant medical devices.

Cited by 10 publications

References 34 publications

Biofilm Growth Causes Damage to Silicone Voice Prostheses in Patients after Surgical Treatment of Locally Advanced Laryngeal Cancer

Biofilm Growth Causes Damage to Silicone Voice Prostheses in Patients after Surgical Treatment of Locally Advanced Laryngeal Cancer

Culture media influences Candida parapsilosis growth, susceptibility, and virulence

Care of Patients With a Laryngectomy During the COVID‐19 Pandemic

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