In vitro biofilm formation in ESBL producing Escherichia coli isolates from cage birds

Yılmaz, Ebru Şebnem; Güvensen, Nur Ceyhan

doi:10.1016/j.apjtm.2016.10.003

Cited by 6 publications

(5 citation statements)

References 42 publications

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“…Besides companion and food producing animals, scattered reports exist on the isolation of ESBL from domestic animals such as wild birds and dromedaries in the Mediterranean. For instance, CTX-M-producing E. coli was isolated from wild birds in Algeria (Meguenni et al, 2015 ), Turkey (Yilmaz and Guvensen, 2016 ), bla CTX-M-1 in addition to bla CTX-M-15 carrying E. cloacae in France (Bonnedahl et al, 2009 ). Furthermore, in France, CTX-M-1 and CTX-M-15 were detected in ST93, ST124, and ST10 E. coli strains recovered from tawny owls/rock pigeons and domestic geese, respectively.…”

Section: Distribution Of Esbls and Ampc Producers In Animalsmentioning

confidence: 99%

Prevalence and Emergence of Extended-Spectrum Cephalosporin-, Carbapenem- and Colistin-Resistant Gram Negative Bacteria of Animal Origin in the Mediterranean Basin

et al. 2018

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In recent years, extended ESBL and carbapenemase producing Gram negative bacteria have become widespread in hospitals, community settings and the environment. This has been triggered by the few therapeutic options left when infections with these multi-drug resistant organisms occur. The emergence of resistance to colistin, the last therapeutic option against carbapenem-resistant bacteria, worsened the situation. Recently, animals were regarded as potent antimicrobial reservoir and a possible source of infection to humans. Enteric Gram negative bacteria in animals can be easily transmitted to humans by direct contact or indirectly through the handling and consumption of undercooked/uncooked animal products. In the Mediterranean basin, little is known about the current overall epidemiology of multi-drug resistant bacteria in livestock, companion, and domestic animals. This review describes the current epidemiology of ESBL, carbapenemase producers and colistin resistant bacteria of animal origin in this region of the world. The CTX-M group 1 seems to prevail in animals in this area, followed by SHV-12 and CTX-M group 9. The dissemination of carbapenemase producers and colistin resistance remains low. Isolated multi-drug resistant bacteria were often co-resistant to non-beta-lactam antibiotics, frequently used in veterinary medicine as treatment, growth promoters, prophylaxis and in human medicine for therapeutic purposes. Antibiotics used in veterinary medicine in this area include mainly tetracycline, aminoglycosides, fluoroquinolones, and polymyxins. Indeed, it appears that the emergence of ESBL and carbapenemase producers in animals is not related to the use of beta-lactam antibiotics but is, rather, due to the co-selective pressure applied by the over usage of non-beta-lactams. The level of antibiotic consumption in animals should be, therefore, re-considered in the Mediterranean area especially in North Africa and western Asia where no accurate data are available about the level of antibiotic consumption in animals.

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Section: Distribution Of Esbls and Ampc Producers In Animalsmentioning

confidence: 99%

Prevalence and Emergence of Extended-Spectrum Cephalosporin-, Carbapenem- and Colistin-Resistant Gram Negative Bacteria of Animal Origin in the Mediterranean Basin

et al. 2018

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“…A possible explanation for the findings is that E. coli can be classified into four commensals phylogenetic groups, two groups were considered as non-pathogenic while the other two groups were virulent. 24 However, the small sample size tested for biofilm formation (n=65) might be another justification. On the other hand, the present study showed that the association between biofilm formation and the bacterial species was highly significant (P <0.001), and the strength of biofilm was significantly higher among K. pneumoniae.…”

Section: Ethical Approvalmentioning

confidence: 99%

Extended-spectrum beta-lactamases (ESBLs) detection in some uropathogenic bacteria and their correlation with biofilm formation

Hassan¹,

Hanna²

2019

Zanco J. Med. Sci.

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A total of 438 urine specimens were collected from Rizgary Teaching hospital in Erbil, Iraq, from September to December 2017. Extended spectrum beta lactamase was determined using the Vitek-2 automated system and confirmed by using the combination disk diffusion test. Biofilm formation was checked using 96-well flat bottom microtiter plates. Results: Out of the 438 urine specimens, only 37.89% (n =166) developed an infection, the most common isolate was Escherichia coli. The distribution of the bacterial species according to the patients' gender found to be significant (P = 0.014). The proportion of betalactamase producing isolates was 29% (n = 31). The strength of biofilm formation among Klebsiella species was significantly higher than in Escherichia coli (P <0.001), while a non-significant difference (P = 0.163) was observed between beta lactamase production and ability of biofilm formation. The sensitivity and specificity of VITEK-2 in the detection of extended-spectrum beta-lactamase were 79.48% and 80.95%, respectively. Conclusion: The study revealed that Klebsiella species were stronger biofilm producers. Beta lactamase producing isolates do not have a greater ability of biofilm formation.

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“…The therapy deficiency and interfered illness control are remarkably assisted with the production of Enterobacteriaceae (Klebsiella pneumoniae and Escherichia coli) through extended-spectrum beta-lactamases (ESBLs) in human and veterinary medicine [1]. Various ESBL genotypes in E. coli are present in the environment, livestock, wild animals, and poultry [2]; however, the formation of biofilm by the organisms produces some of the cell surface constituents and increases endurance to multiple antimicrobials for promoting their availability in different communities [3].…”

Section: Introductionmentioning

confidence: 99%

“…The removal of planktonic cells results in the progression of circ and colonizes different surfaces. Hydrophobic interactions play important roles in adhering to and forming biofilm [1].…”

Section: Introductionmentioning

confidence: 99%

Interrelationship of Extended Spectrum Beta-Lactamase Producers and Biofilm Formation among the Gram-Negative Bacteria from Tabuk, KSA

Zubair

Mohammad²

2023

Open Access Maced J Med Sci

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AIM: The present study investigates the production of extended-spectrum beta-lactamases (ESBL) and the formation of biofilm among different bacterial pathogens. METHODS: The study conducted prospective analysis on bacteria isolates (Gram-negative) from patients who have diagnosed with infections with bacteria between October 2020 and January 2022. RESULTS: The results showed that there were 53 biofilm producers in Escherichia coli. In contrast, Pseudomonas aeruginosa was observed to have the highest percentage, with 32/40 (80%) isolates being biofilm producers. The least number of isolates were Morganella morganii (n = 2) with two (100%) biofilm producers. The resistance in the biofilm positive isolates was high compared with biofilm negative. About 88% of phenotypic ESBL-positive isolates were biofilm producers, and 97% of cefotaxime-resistant biofilm-positive isolates were genotypic positive for CTX-M, TEM, and SHV genes. CONCLUSION: The present study has shown that protection against antibiotics through mucus production is possible due to bacteria’s reduced metabolic activity and diffusion of antibiotics across the biofilm matrix. In this study, all the bacterial strains of E. coli and Klebsiella pneumoniae were reported to be MDR and competent for establishing biofilm.

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In vitro biofilm formation in ESBL producing Escherichia coli isolates from cage birds

Cited by 6 publications

References 42 publications

Prevalence and Emergence of Extended-Spectrum Cephalosporin-, Carbapenem- and Colistin-Resistant Gram Negative Bacteria of Animal Origin in the Mediterranean Basin

Prevalence and Emergence of Extended-Spectrum Cephalosporin-, Carbapenem- and Colistin-Resistant Gram Negative Bacteria of Animal Origin in the Mediterranean Basin

Extended-spectrum beta-lactamases (ESBLs) detection in some uropathogenic bacteria and their correlation with biofilm formation

Interrelationship of Extended Spectrum Beta-Lactamase Producers and Biofilm Formation among the Gram-Negative Bacteria from Tabuk, KSA

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