In vitro biocompatibility assessment of poly($epsiv;-caprolactone) films using L929 mouse fibroblasts

Serrano, María Concepción; Pagani, Raffaella; Vallet‐Regí, María; Peña, J. L.; Rámila, Ainhoa; Izquierdo, Isabel; Portolés, María Teresa

doi:10.1016/j.biomaterials.2004.01.037

Cited by 261 publications

(157 citation statements)

References 20 publications

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“…The registered effect may be explained by increased redox activity of mitochondria in the presence of PCL. Similar results were also observed by other authors when culturing L929 fibroblasts on PCL thin films for a short period of time [46] or in the presence of polymeric materials [47].…”

Section: Cytotoxicity Evaluationsupporting

confidence: 90%

Synthesis of amphiphilic [PEO(PCL)2] triarm star-shaped block copolymers: a promising system for in cell delivery

Petrova

Kolev

Miloshev

et al. 2012

J Mater Sci: Mater Med

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The paper reports on a simple method of synthesizing [PEO(PCL)(2)] triarm star-shaped copolymers by a combination of Michael-addition type reaction and ring-opening polymerization. A Michael-addition reaction yielded a PEO end-capped by two hydroxyl groups-a [PEO(OH)(2)] macroinitiator-which was used for sequential building of PCL blocks. The macroinitiator and copolymers were analyzed by FTIR, (1)H NMR spectroscopy and SEC. The self-assembly behavior of the copolymers in aqueous media was studied by UV-Vis spectroscopy. The size and morphology of the obtained micelles were determined by TEM. None of the polymers had cytotoxic effects in vitro. Cellular uptake studies showed the accumulation of neutral red loaded micelles in the perinuclear area of human hepatocellular carcinoma cells revealing a cellular uptake associated with macropinocytosis and caveolae mediated endocytosis. The accumulation had a sustained effect over 3 days pointing at the potential application of the copolymers micelles as a drug delivery system.

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Section: Cytotoxicity Evaluationsupporting

confidence: 90%

Synthesis of amphiphilic [PEO(PCL)2] triarm star-shaped block copolymers: a promising system for in cell delivery

Petrova

Kolev

Miloshev

et al. 2012

J Mater Sci: Mater Med

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“…Moreover, a stimulation of mitochondrial redox activity produced by the contact with a polymer was observed [25]. Exposure of cells to various compounds may interfere with their living systems and result in cytotoxic effect [26].…”

Section: Discussionmentioning

confidence: 98%

Oxidative Stress Parameters of L929 Cells Cultured on Plasma-Modified PDLLA Scaffolds

Demirbilek

Karahaliloğlu

et al. 2011

Appl Biochem Biotechnol

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Oxidative stress may produce high level of reactive oxygen species (ROS) following cell exposure to endogenous and exogenous factors. Recent experiments implicate oxidative stress as playing an essential role in cytotoxicity of many materials. The aim of this study was to measure intracellular malondialdehyde (MDA), advanced oxidation protein product (AOPP) levels, and superoxide dismutase (SOD) activities of L929 fibroblasts cultured on PDLLA, polyethylene glycol (PEG), or ethylenediamine (EDA) grafted PDLLA by plasma polymerization method. Cell proliferation on these scaffolds was studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. The study showed that MDA, AOPP levels, and SOD activities in L929 fibroblast cells cultured on all scaffolds were significantly different compared to the control group and each other. The highest MDA (0.42 ± 0.76 nmol/mg protein), AOPP (14.99 ± 4.67 nmol/mg protein) levels, and SOD activities (7.49 ± 3.74 U/mg protein) were observed in cells cultured on non-modified scaffolds; meanwhile, the most cell proliferation was obtained in EDA-modified scaffolds (MDA 0.15 ± 0.14 nmol/mg protein, AOPP 13.12 ± 3.86 nmol/mg protein, SOD 4.82 ± 2.64 U/mg protein). According to our finding, EDA- or PEG-modified scaffolds are potentially useful as suitable biomaterials in tissue engineering.

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“…Presently, the L929 cell line is widely used to evaluate the cytotoxicity of materials (Torabinejad et al 1995;Kaga et al 2001;Serrano et al 2004;Scharnweber et al 2008) based on the requirement of ISO 10993 (International Standards Organization 10993 1992). Testing by this standard, 45S5 Bioglass has been widely reported to possess excellent biocompatibility (Shirtliff & Hench 2003;Wilson et al 2004;Jones & Hench 2006).…”

mentioning

confidence: 99%

Strontium borate glass: potential biomaterial for bone regeneration

Pan

Zhao

Zhang

et al. 2009

J. R. Soc. Interface.

146

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Boron plays important roles in many life processes including embryogenesis, bone growth and maintenance, immune function and psychomotor skills. Thus, the delivery of boron by the degradation of borate glass is of special interest in biomedical applications. However, the cytotoxicity of borate glass which arises with the rapid release of boron has to be carefully considered. In this study, it was found that the incorporation of strontium into borate glass can not only moderate the rapid release of boron, but also induce the adhesion of osteoblast-like cells, SaOS-2, thus significantly increasing the cyto-compatibility of borate glass. The formation of multilayers of apatite with porous structure indicates that complete degradation is optimistic, and the spread of SaOS-2 covered by apatite to form a sandwich structure may induce bone-like tissue formation at earlier stages. Therefore, such novel strontium-incorporated borosilicate may act as a new generation of biomaterial for bone regeneration, which not only renders boron as a nutritious element for bone health, but also delivers strontium to stimulate formation of new bones.

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In vitro biocompatibility assessment of poly($epsiv;-caprolactone) films using L929 mouse fibroblasts

Cited by 261 publications

References 20 publications

Synthesis of amphiphilic [PEO(PCL)2] triarm star-shaped block copolymers: a promising system for in cell delivery

Synthesis of amphiphilic [PEO(PCL)2] triarm star-shaped block copolymers: a promising system for in cell delivery

Oxidative Stress Parameters of L929 Cells Cultured on Plasma-Modified PDLLA Scaffolds

Strontium borate glass: potential biomaterial for bone regeneration

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