In vitro bioassays for the study of endocrine-disrupting food additives and contaminants

Connolly, Lisa; Ropstad, Erik; Verhaegen, Steven

doi:10.1016/j.trac.2010.10.009

Cited by 41 publications

(15 citation statements)

References 81 publications

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“…The method utilizes laser to fluorescently induce the antibodies that is specifically bound to analyte. Similarly to Endotect TM the evanescent response around the glass fiber is obtained and quantitatively measured [35,42].…”

Section: Endotect Tmmentioning

confidence: 99%

See 1 more Smart Citation

Endocrine Disrupting Compounds – Problems and Challenges

Kudłak¹,

Szczepańska²,

Owczarek³

et al. 2015

Emerging Pollutants in the Environment - Current and Further Implications

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In this chapter, information about some of the estrogenic compounds and their environmental fate and biological influence can be found. Special attention is paid to the review of the analytical approaches used at the stages of detection and determination of Endocrine Disrupting Compounds (EDCs) in the environmental samples. Also, a brief characterization of both cellular and non-cellular bioassays is presented. The discovery of micropollutants occurring in the environment resulted in new methodologies being put into the analytical practice. These methodologies are developed in two different directions. The first is based on methodological solutions designed to detect, identify, and determine xenobiotics that occur in various environmental samples. For this purpose, instrumental methods such as gas and liquid chromatography with mass spectrometry detection are usually used. The second approach is to put into the analytical practice the new bioanalytical methodologies. These methodologies allow the estimation of the sample endocrine potential, but they do not provide the information about which of the sample ingredient is responsible for causing the toxic effect. These results can be the basis for estimating the endocrine potential of the environment exhibited by certain species. Moreover, bioanalytical techniques may be supplementary to the techniques of quantitative and qualitative determination of EDCs.

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Section: Endotect Tmmentioning

confidence: 99%

“…For example, the estrogen gene can be introduced to the yeast cells from human, fish, or other species genome. In such case, the term of estrogen equivalent concentration (EEC) finds its application in the form of the formula [42]:…”

Section: Biological Cellular Tests Used To Assess the Endocrine Potenmentioning

confidence: 99%

Endocrine Disrupting Compounds – Problems and Challenges

Kudłak¹,

Szczepańska²,

Owczarek³

et al. 2015

Emerging Pollutants in the Environment - Current and Further Implications

View full text Add to dashboard Cite

show abstract

“…However, results can be confounded by cell expression of other receptors (such as AR and glucocorticoid receptor) that induce non-specific cell proliferation. The assay relies on cell growth and, therefore, it can take days to produce results [48]. Thus, it is not really feasible for use in sport doping laboratories.…”

Section: Androgen Bioassaysmentioning

confidence: 99%

In Vitro Androgen Bioassays as a Detection Method for Designer Androgens

Cooper

McGrath

Heather

2013

Sensors

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Androgens are the class of sex steroids responsible for male sexual characteristics, including increased muscle mass and decreased fat mass. Illicit use of androgen doping can be an attractive option for those looking to enhance sporting performance and/or physical appearance. The use of in vitro bioassays to detect androgens, especially designer or proandrogens, is becoming increasingly important in combating androgen doping associated with nutritional supplements. The nutritional sports supplement market has grown rapidly throughout the past decade. Many of these supplements contain androgens, designer androgens or proandrogens. Many designer or proandrogens cannot be detected by the standard highly-sensitive screening methods such as gas chromatography-mass spectrometry because their chemical structure is unknown. However, in vitro androgen bioassays can detect designer and proandrogens as these assays are not reliant on knowing the chemical structure but instead are based on androgen receptor activation. For these reasons, it may be advantageous to use routine androgen bioassay screening of nutraceutical samples to help curb the increasing problem of androgen doping.

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“…However, for screening purposes, assays based on the detection of bioactivity are advantageous [21][22][23] because they have the ability to biologically detect both known and unknown EDs as well as their low-level mixtures. One such assay that shows great potential is the reporter gene assay (RGA) [24].…”

Section: Introductionmentioning

confidence: 99%

Validation and application of reporter gene assays for the determination of estrogenic and androgenic endocrine disruptor activity in sport supplements

et al. 2012

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Previously developed estrogen and androgen mammalian reporter gene assays (RGAs) were assessed for their potential use as a quantitative screening method in the detection of estrogenic and androgenic endocrine disruptors (EDs) in sport supplements. The validation of both RGAs coupled with dispersive solid phase extraction (dSPE) was performed in accordance with European Commission Decision EC/2002/6579 for biological screening methods. Decision limits (CCα) and detection capabilities (CCβ) were established for both the estrogen and androgen RGAs. All samples were compliant with CCα and CCβ in both bioassays. Recovery rates were 96 % for 17β-estradiol and 115 % for dihydrotestosterone as obtained in their corresponding RGA. Both estrogens and androgens were stable in samples for more than 3 weeks, when stored at -20 °C. Specificity, good repeatability (coefficients of variation (CV), 12-25 %), reproducibility and robustness of both bioassays were also observed. Four different ED modes of action were determined for estrogens and androgens in 53 sport supplements, using the validated RGAs. This study revealed that 89 % of the investigated sport supplements contained estrogenic EDs and 51 % contained androgenic compounds. In conclusion, both bioassays are suitable for sport supplement screening of estrogenic and androgenic EDs.

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In vitro bioassays for the study of endocrine-disrupting food additives and contaminants

Cited by 41 publications

References 81 publications

Endocrine Disrupting Compounds – Problems and Challenges

Endocrine Disrupting Compounds – Problems and Challenges

In Vitro Androgen Bioassays as a Detection Method for Designer Androgens

Validation and application of reporter gene assays for the determination of estrogenic and androgenic endocrine disruptor activity in sport supplements

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