In vitro bioactivity and phytochemical analysis of two marine macro-algae

Cyril, Ravi; Lakshmanan, Revathi; Thiyagarajan, Amuthavalli

doi:10.12980/jclm.5.2017j7-124

Cited by 10 publications

(7 citation statements)

References 23 publications

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“…Berdasarkan penelitian yang dilakukan oleh Widowati et al (2014) dan Cyril et al (2017), Gracilaria verrucosa mengandung metabolit sekunder seperti alkaloid, flavonoid, terpenoid serta senyawa bioaktif lainnya. Alkaloid, flavonoid, dan terpenoid merupakan senyawa bioaktif yang dapat berfungsi sebagai antijamur dan antioksidan (Saxena et al, 2013 Lestario et al (2008) yang memperoleh fenolat total sebesar 20,01 mg GAE/ g sampel untuk ekstrak metanol namun lebih besar dari penelitian yang dilakukan oleh Widowati et al (2014) dimana ekstrak Gracilaria verrucosa memiliki kandungan fenolat total sebesar 7,86 mg GAE / g sampel ekstrak metanol.…”

unclassified

Potensi Antioksidan Rumput Laut Gracilaria verrucosa Dari Pantai Gunung Kidul, Yogyakarta

Febrianto¹,

Djunaedi²,

Suryono³

et al. 2019

J. Kel. Trop.

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Gracilaria verrucosa is red algae that has been widely used as an antioxidant. This research was conducted to test antioxidant activity, total phenolic content and bioactive compound of Gracilaria verrucosa obtained from Pok Tunggal Beach and Ngandong Beach, Gunung Kidul, Yogyakarta. Research was carried out by descriptive method. Samples were fresh and taken from the beach, then macerated for 3x24 hours in a methanol solvent. Antioxidant test was carried out by electron transfer method with DPPH 0.1 mM and measurement of antioxidant activity using. Total phenolic contents were measured using the Folin-ciocalteau method using gallic acid standard on 725 nm wavelength. The phytochemical content observated by changing of extract color by reagent. Pigment contents were measured using spectrophotometric methods at wavelengths 636 and 663 (chlorophyll-a and chlorophyll-b) and 480 nm (carotenoids). The results showed that IC50 value extract of Pok Tunggal Beach and Ngandong Beach were 188,53 ppm and 168,76 ppm. Phenolic content of each extract were 16,527 and 17,497 mg GAE / g sample weight). Chlorophyll-a levels were 7,132 and 4,357 mg/g, chlorophyll-b were 8,335 and 5,401 mg/g, carotenoids were 31,625 and 35,494 µmol/g. Gracilaria verrucosa from Ngandong Beach have antioxidant activity.Gracilaria verrucosa merupakan alga merah yang pemanfaatannya sudah banyak dilakukan sebagai antioksidan. Penelitian ini bertujuan untuk mengetahui potensi antioksidan, menghitung kadar fenolat total, dan senyawa bioaktif yang terkandung pada Gracilaria verrucosa yang diperoleh dari Pantai Pok Tunggal dan Pantai Ngandong, Gunung Kidul, Yogyakarta. Penelitian ini dilakukan dengan metode deskriptif. Sampel segar diambil langsung dari pantai, kemudian dimaserasi selama 3x24 jam dalam pelarut metanol. Uji antioksidan dilakukan dengan metode transfer elektron dengan DPPH 0,1 mM dan pengukuran aktivitas antioksidan menggunakan perhitungan nilai IC50. Kadar fenolat total diukur menggunakan metode Folin-ciocalteau dengan asam galat sebagai standar pada panjang gelombang 725 nm. Kandungan fitokimia diuji menggunakan pengamatan perubahan warna ekstrak saat diberikan pereaksi. Kadar pigmen diukur menggunakan metode spektrofotometri pada panjang gelombang 636, 663 (klorofil-a dan klorofil-b) dan 480 nm (karotenoid). Hasil menunjukkan bahwa nilai IC50 ekstrak sampel dari Pantai Pok Tunggal dan Pantai Ngandong berturut-turut adalah 188,53 ppm dan 168,76 ppm. Kadar fenolat masing-masing ekstrak sebesar 16,527 dan 17,497 mg GAE/g berat sampel). Kadar klorofil-a sebesar 7,132 dan 4,357 mg/g, klorofil-b sebesar 8,335 dan 5,401 mg/g, karotenoid sebesar 31,625 dam 35,494 µmol/g. Gracilaria verrucosa dari Pantai Ngandong dan Pantai Pok Tunggal memiliki potensi antioksidan.

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unclassified

Potensi Antioksidan Rumput Laut Gracilaria verrucosa Dari Pantai Gunung Kidul, Yogyakarta

Febrianto¹,

Djunaedi²,

Suryono³

et al. 2019

J. Kel. Trop.

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“…Antioksidan jenis ini disebut sebagai antioksidan alami dan jumlahnya terbatas (Sayuti dan Yenrina, dalam Yadav et al, 2016). Penelitian yang dilakukan oleh Widowati et al, (2014) dan Cyril et al, (2017) bahwa E. spinosum mengandung metabolit sekunder seperti alkaloid, flavonoid, terpenoid serta senyawa bioaktif lainnya. Alkaloid, flavonoid dan terpenoid merupakan senyawa bioaktif yang dapat berfungsi sebagai anti jamur dan antioksidan Saxena et al, (2013).…”

Section: Pendahuluanunclassified

KARAKTERISTIK MUTU SIRUP RUMPUT LAUT (Eucheuma spinosum)

Anharullah

Haslianti²,

Asyik³

2021

Fish, Protech

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The aim of this study was to determine the quality characteristics of seaweed Syrup (Eucheuma spinosum). This study used a Completely Randomized Design (CRD) consisting of 3 treatments, namely: P1 = 100 gr seaweed: 200 gr sugar: 3.5 L water: CMC 0.3 gr, P2 = 120 gr seaweed: 200 granulated sugar gr: water 3,5 L: CMC 0,3 gr, P3 = Seaweed 140 gr: sugar 200 gr: water 3,5 L: CMC 0,3 gr: with three times replications. Data from observations were analyzed using ANOVA (Analysis of Variance) at a level of 95%, if there is a significant difference (P> 0.05), further tests were carried out with the DMRT (Duncan Multiple Range Test) tests at a 95% confidence level. The results obtained showed that the concentration of seaweed (E. spinosum), water, and taste did not significantly affect the aroma and taste of seaweed syrup. The best treatment was in the treatment of P1 with a scent value of 3.46 with a rather like category, and P1 for a taste of 3.46 with a somewhat like category. The chemical value of seaweed syrup is the value of water content ranging from 38.96% -43.21%, ash content ranges from 0.82-1.34%. Keywords: Syrup, seaweed (Eucheuma spinosum), chemistry and sensoryABSTRAKTujuan penelitian ini adalah untuk mengetahui Karakteristik Mutu Sirup Rumput Laut (Eucheuma spinosum). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 3 perlakuan yaitu : P1 = Rumput laut 100 gr : gula pasir 200 gr : air 3,5 L : CMC 0,3 gr, P2 = Rumput laut 120 gr : gula pasir 200 gr: air 3,5 L : CMC 0,3 gr, P3 = Rumput laut 140 gr : gula pasir 200 gr : air 3,5 L : CMC 0,3 gr : dan ulangan sebanyak tiga kali. Data hasil pengamatan dianalisa menggunakan ANOVA (Analysis of Variance) pada taraf 95%, apabila terdapat beda nyata (P>0,05) maka dilakukan uji lanjut dengan uji DMRT (Duncan Multiple Range Test) pada taraf kepercayaan 95%. Hasil penelitian yang diperoleh menunjukkan bahwa Kosentrasi rumput laut (E. Spinosum), air dan rasa berpengaruh tidak nyata terhadap aroma dan rasa sirup rumput laut. Perlakuan terbaik terdapat pada perlakuan P1 dengan nilai aroma sebesar 3,46 dengan kategori agak suka, dan P1 rasa 3,46 dengan kategori agak suka. Nilai kimia sirup rumput laut yaitu nilai kadar air berkisar 38,96%-43,21%, kadar abu berkisar 0,82-1,34%.Kata kunci: Sirup, rumput laut (Eucheuma spinosum), kimia dan sensori

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“…12 The polarity, concentration, and physiochemical characteristics of solvents will affect the activity of bioactive compounds present in the seaweed. 13 A study done in Jakarta, Indonesia performed phytochemical analysis on seaweed G. verrucosa which also comes from the same habitat studied here. The phytochemical result was positive for saponin and triterpenoid.…”

Section: Cytotoxic Activity Of Seaweeds G Verrucosamentioning

confidence: 99%

In vitro Evaluation of Seaweed Gracilaria verrucosa for Cytotoxic Activity against Cervical HeLa Cells

Dewi

Arsianti²,

Zagloel

et al. 2018

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Background: Seaweed macroalgae of Gracilaria verrucosa has been known to have a potent anticancer activity, however the cytotoxicity against cervical cancer has not been explored further. Objective: This study aims to utilize Indonesia's marine resource which is focused on seaweed macroalgae G. verrucosa as a future anti-cervical cancer agent. Materials and Method: Seaweed G. verrucosa originated from Labuan Aji beach, Nusa Tenggara Barat, Indonesia, extracted, macerated, and fractionated into four organic solvents of different polarity, consisting of hexane, ethyl acetate, chloroform and ethanol. Then, the macroalgae extracts are diluted into 8 different concentrations. Afterwards, in vitro anticancer activity evaluation of hexane, ethyl acetate, chloroform and ethanol extracts of G. verrucosa against cervical HeLa cells were conducted by MTT cell proliferation assay. Triplo mechanism is also applied in this study to increase the accuracy of the results. The anticancer activity is measured using IC 50 value. Results: The four concentrated extracts G. verrucosa showed cytotoxicity against cervical HeLa cells. The greatest anticancer activity is depicted by hexane extract with an IC 50 of 14.94 µg/mL, followed by chloroform (IC 50 15.74 µg/mL), ethyl acetate (IC 50 16.18 µg/mL), and ethanol (IC 50 19.43 µg/mL). Conclusion: Our results clearly indicate that hexane, ethanol, chloroform, and ethyl acetate extracts of seaweed G. verrucosa can be further developed to be anti-cervical cancer agents, with hexane extract displaying the greatest cytotoxic effect.

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In vitro bioactivity and phytochemical analysis of two marine macro-algae

Cited by 10 publications

References 23 publications

Potensi Antioksidan Rumput Laut Gracilaria verrucosa Dari Pantai Gunung Kidul, Yogyakarta

Potensi Antioksidan Rumput Laut Gracilaria verrucosa Dari Pantai Gunung Kidul, Yogyakarta

KARAKTERISTIK MUTU SIRUP RUMPUT LAUT (Eucheuma spinosum)

In vitro Evaluation of Seaweed Gracilaria verrucosa for Cytotoxic Activity against Cervical HeLa Cells

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