In vitro binding interaction of atorvastatin with calf thymus DNA: multispectroscopic, gel electrophoresis and molecular docking studies

Mirzaei-Kalar, Zeinab

doi:10.1016/j.jpba.2018.08.033

Cited by 47 publications

(10 citation statements)

References 57 publications

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“…The closed‐loop supercoiled DNA (Form I) moves closer to the front through the gel due to its tight structure, and the linear material (Form III) moves at an intermediate rate. The open‐loop notch‐type DNA (Form II) moves slowly due to its loose structure, and its movement towards the positive electrode is suppressed . As shown in Figure (d), under the same conditions, 2b can convert pBR322 from Form I to Form II, and the degree of DNA fracture increased with increasing concentration of 2b .…”

Section: Resultsmentioning

confidence: 87%

“…The activity depends The open-loop notch-type DNA (Form II) moves slowly due to its loose structure, and its movement towards the positive electrode is suppressed. [59][60][61] As shown in Figure 3 (d), under the same conditions, 2b can convert pBR322 from Form I to Form II, and the degree of DNA fracture increased with increasing concentration of 2b. Greater concentrations of 2b resulted in greater degrees of DNA fragmentation.…”

Section: Gel Electrophoresismentioning

confidence: 99%

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Microwave assisted synthesis of disubstituted benzyltin arylformylhydrazone complexes: anticancer activity and DNA‐binding properties

Jiang

Zhou

Liu

et al. 2019

Applied Organom Chemis

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Eight disubstituted benzyltin complexes, i.e., {[R(O)C=N‐N=C (Me)COO]R'2Sn(CH3OH)}n (1a and 2b), {[R(O)C=N‐N=C (Me)COO]R'2Sn(CH3OH)}2 (1b and 1d) and {[R(O)C=N‐N=C (Me)COO]R'2Sn}n (1c, 2a, 2c, and 2d) (R = C4H3O‐, C4H3S‐, p–t‐Bu‐C6H4‐ or p‐MeO‐C6H4‐; R' = o‐Cl‐C6H4CH2‐ or o‐Me‐C6H4CH2‐), were prepared from the reaction of arylformylhydrazine, pyruvic acid and disubstituted benzyltin dichloride with microwave irradiation. All complexes were characterized by FT‐IR spectroscopy, 1H, 13C and 119Sn NMR spectroscopy, HRMS, elemental analysis, X‐ray single‐crystal diffraction and TGA. The in vitro antitumour activities of all complexes were evaluated by an MTT assay against three human cancer cell lines (NCI‐H460, HepG2, and MCF7). 2b exhibited strong antitumour activity on HepG2 cells and was expected to be a suitable platform for further chemical optimization to develop as anticancer therapeutics. The DNA binding of 2b was studied by UV–visible absorption spectrometry, fluorescence competitive assays, viscosity measurements and gel electrophoresis. Molecular docking was used to predict the binding between 2b and DNA, and the results show that 2b can become embedded in the double helix of DNA and cleave DNA.

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Section: Resultsmentioning

confidence: 87%

Section: Gel Electrophoresismentioning

confidence: 99%

Microwave assisted synthesis of disubstituted benzyltin arylformylhydrazone complexes: anticancer activity and DNA‐binding properties

Jiang

Zhou

Liu

et al. 2019

Applied Organom Chemis

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“…replacement of dye form the DNA helix is based on the principle that the drug is expected to bind at the same position where the dye was bound. 37,38 Therefore, the FI dye-ctDNA system is expected to have some change on the addition of drug that is supposed to bind similarly. Ethidium bromide is a well-known probe dye used in the investigation of intercalative binding analysis.…”

Section: Competitive Displacement Assaymentioning

confidence: 99%

A Spectroscopic, Thermodynamic and Molecular Docking Study of the Binding Mechanism of Dapoxetine with Calf Thymus DNA

et al. 2020

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Dapoxetine is a selective serotonin reuptake inhibitor, used to treat premature ejaculation in men. Dapoxetine may interact with the DNA and hence this study investigated dapoxetine and calf thymus DNA (ctDNA) binding interaction. The interaction study of ligands to DNA is of importance in the development of molecular probes and therapeutic agents. Spectroscopic techniques including spectrofluorometry and spectrophotometry were employed to study this interaction. Fluorescence studies indicated a static quenching mechanism between dapoxetine and ctDNA. Groove binding was suggested as the mode of interaction between dapoxetine and ctDNA based on UV absorption, circular dichroism (CD) spectroscopy, iodide quenching and molecular docking studies. The studies conducted at three different temperatures 298, 303 and 310 K indicated a strong binding interaction at higher temperatures. Thermodynamic studies conducted indicated involvement of hydrophobic interaction between ctDNA and dapoxetine and were entropy-driven. Ethidium bromide probe study suggested that dapoxetine does not bind to ctDNA in an intercalative fashion. Iodide quenching studies further proved the non-intercalative binding of ctDNA with dapoxetine. Ionic strength studies conducted ruled out the electrostatic binding mechanism between ctDNA and dapoxetine. Molecular docking analysis performed for the dapoxetine with calf thymus DNA (ctDNA) interaction and confirmed minor groove binding of dapoxetine to ctDNA. The study helped to reveal the binding interaction mechanism between dapoxetine and ctDNA.

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“…Electrophoresis was conducted for approximately 30 min at 120 V (DYY-6C, Beijing Liuyi Co. Ltd., Beijing, China). DNA bands in the gel were imaged under a UV transilluminator [41].…”

Section: Protective Effect Against Dna Damagementioning

confidence: 99%

DNA Binding Characteristics and Protective Effects of Yellow Pigment from Freshly Cut Yam (Dioscorea opposita)

Zhao

Zhao²,

Ma³

et al. 2020

Molecules

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Yam yellow pigments (YP) are natural pigments formed during the storage of freshly cut yam (Dioscorea opposita) under certain conditions. The interaction of YP with calf thymus DNA (ctDNA) and its protective effect against DNA oxidative damage were investigated using multiple spectroscopic techniques, competitive binding experiments, viscosity measurements, and gel electrophoresis. Results showed that YP participated in intercalative binding with ctDNA. YP exhibited a protective effect against hydroxyl-induced DNA damage, which was attributed to the high hydroxyl radical scavenging activity of YP. Our findings improve our understanding of the mechanism of interaction between YP and ctDNA, and provide a theoretical basis for the application of YP in the food and drug industry.

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In vitro binding interaction of atorvastatin with calf thymus DNA: multispectroscopic, gel electrophoresis and molecular docking studies

Cited by 47 publications

References 57 publications

Microwave assisted synthesis of disubstituted benzyltin arylformylhydrazone complexes: anticancer activity and DNA‐binding properties

Microwave assisted synthesis of disubstituted benzyltin arylformylhydrazone complexes: anticancer activity and DNA‐binding properties

A Spectroscopic, Thermodynamic and Molecular Docking Study of the Binding Mechanism of Dapoxetine with Calf Thymus DNA

DNA Binding Characteristics and Protective Effects of Yellow Pigment from Freshly Cut Yam (Dioscorea opposita)

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