In Vitro Behavior of Human Nonfertile Semen in Isoosmotic and Hyperosmotic Media

Pilikian, S.; Moncharmont, P.; Pinatel, M. C.; Guérin, J. F.; Mathieu, C.; Lornage, Jacqueline

doi:10.3109/01485018708988483

Cited by 5 publications

(3 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, the fertilizability of spermatozoa appears to be higher in hypertonic rather than in hypotonic solution in the rabbit [3], mouse, hamster [10] and man [13]. However, little is known regarding the effects of NaCl concentration in fertilization medium on bovine sperm penetration and subsequent in vitro development of bovine embryos.…”

mentioning

confidence: 99%

Improved Monospermic Fertilization and Subsequent Blastocyst Formation of Bovine Oocytes Fertilized In Vitro in a Medium Containing NaCl of Decreased Concentration.

et al. 2002

View full text Add to dashboard Cite

ABSTRACT. The objective of this study was to evaluate whether changes in NaCl concentration in a fertilization medium could improve normal fertilization and preimplantation development of bovine oocytes. In vitro matured bovine oocytes were inseminated with frozenthawed semen for 18 hr in a Tyrode's medium with albumin, lactate and pyruvate (TALP), to which 114 (TALP-114), 96 (TALP-96) or 78 (TALP-78) mM NaCl was added. Presumptive zygotes were cultured for 192 hr in a modified TALP containing 90 mM NaCl, 1.5 mM glucose, 0.3% (w/v) BSA, minimal essential medium (MEM) essential and nonessential amino acids, and insulin-transferrinselenium complex. Lower polyspermy rate was obtained by the insemination in TALP-96 (7.8 ± 2.3%) than by the insemination in TALP-114 (25.6 ± 1.4%), without decrease in male pronucleus (MPN) formation. Fertilization in TALP-78 also yielded decreased polyspermic fertilization (3.8 ± 1.5%), but significant decrease in MPN formation was found (63.1 ± 3.1%). In preimplantation development, more blastocysts developed from oocytes inseminated in TALP-96 (24.1 ± 1.7%) than from oocytes inseminated in TALP-114 (16.8 ± 1.4%). TALP-78, however, did not improve preimplantation development beyond the 8-cell stage compared with TALP-114. Mean cell number of blastocyst was higher when oocytes were fertilized in TALP-96 (137.0 ± 4.5) than in TALP-114 (123.1 ± 5.1) and in ). These results demonstrate that insemination of bovine oocytes in a TALP with decreased NaCl concentration (96 mM) improves blastocyst formation and embryo viability. Decrease in NaCl concentration below 96 mM, however, may delay or inhibit MPN formation, and inhibits subsequent development in vitro. KEY WORDS: bovine, embryo development, in vitro fertilization, osmolarity, sodium chloride.

show abstract

mentioning

confidence: 99%

Improved Monospermic Fertilization and Subsequent Blastocyst Formation of Bovine Oocytes Fertilized In Vitro in a Medium Containing NaCl of Decreased Concentration.

et al. 2002

View full text Add to dashboard Cite

show abstract

“…The penetration rate of human spermatozoa in zona-free hamster oocytes most probably also reflects this efficiency, as these oocytes can only be penetrated by fully acrosome-reacted spermato zoa [1,6]. Incubation of human spermatozoa in media exhibiting osmolalities of 410 [6] and 420 mosm/kg [11] (obtained by adding NaCl to the medium) has been shown to enhance the capacitation process. This was indicated by higher proportions of free-swimming sper matozoa exhibiting an AR and higher penetration rates of zona-free hamster oocytes.…”

Section: Discussionmentioning

confidence: 99%

“…Hyperos motic media have been shown to be even more effective for the capacitation of human spermatozoa as demon strated by higher rates of free-swimming, spontaneously acrosome-reacted spermatozoa and higher penetration rates in the zona-free hamster oocyte test compared to spermatozoa capacitated in normosmotic media [6,11].…”

Section: Introductionmentioning

confidence: 99%

Human in vitro Fertilization Using Spermatozoa Capacitated in Hyperosmotic Media

Dumoulin

Evers

Offermans

et al. 1990

Gynecol Obstet Invest

View full text Add to dashboard Cite

In two separate prospective randomized series of 38 and 16 attempts of human in vitro fertilization (IVF), respectively, the fertilizing abilities of human spermatozoa capacitated in hyperosmotic media and in normosmotic medium were compared. Oocytes from each IVF attempt were divided into two equal groups and inseminated with spermatozoa incubated for 2h in medium with an osmolality of 285 mosm/kg or in hyperosmotic medium of 315 (first experiment) or 345 mosm/kg (second experiment). The fertilizing capacity of the spermatozoa was not affected by incubation in medium of 315 mosm/kg as compared to spermatozoa incubated in medium of 285 mosm/kg. However, the fertilizing capacity of the spermatozoa incubated in medium of 345 mosm/kg was significantly reduced. The present study indicates that there is no advantage in the use of hyperosmotic media of 315 or 345 mosm/kg for capacitation of spermatozoa in human IVF.

show abstract

The Andrology Laboratory in an Assisted Reproductive Technologies Program

MULLER

1992

Journal of Andrology

View full text Add to dashboard Cite

Assuring the highest possible quality of care to the patient is the first goal of the andrology laboratory. Quality control and quality assurance as they apply to the andrology laboratory are outlined, and special problems in quality control for sperm function assays are addressed. An example of the patient review process is presented. Quality of care also depends on the ability of the laboratory to perform appropriate tests of sperm function that can diagnose sperm defects, predict success or failure of in vitro fertilization, and lead to rational therapies for sperm dysfunctions. Methods for, and diagnostic value of, sperm swim‐out, enhanced sperm penetration assays, acrosome reaction assessment, motility analysis of capacitating sperm, and sperm—zona binding assays are reviewed. No single test, other than in vitro fertilization itself, is capable of providing a complete diagnosis or a highly accurate prediction. A battery of sperm function tests, arranged in an algorithm, is presented as a theoretically better approach.

show abstract

In Vitro Behavior of Human Nonfertile Semen in Isoosmotic and Hyperosmotic Media

Cited by 5 publications

References 11 publications

Improved Monospermic Fertilization and Subsequent Blastocyst Formation of Bovine Oocytes Fertilized In Vitro in a Medium Containing NaCl of Decreased Concentration.

Improved Monospermic Fertilization and Subsequent Blastocyst Formation of Bovine Oocytes Fertilized In Vitro in a Medium Containing NaCl of Decreased Concentration.

Human in vitro Fertilization Using Spermatozoa Capacitated in Hyperosmotic Media

The Andrology Laboratory in an Assisted Reproductive Technologies Program

Contact Info

Product

Resources

About