In Vitro Autoradiographic Visualization of Guanosine‐5’‐<i>O</i>‐(3‐[<sup>35</sup>S]Thio)triphosphate Binding Stimulated by Sphingosine 1‐Phosphate and Lysophosphatidic Acid

Waeber, Christian; Chiu, Mary L.

doi:10.1046/j.1471-4159.1999.0731212.x

Cited by 38 publications

(34 citation statements)

References 40 publications

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“…Treatment with NEM therefore reveals if members of the G i/o class of G proteins are involved in certain signalling processes. In [ 35 S]GTPgS autoradiography studies, both basal signal and agonist-induced responses are sensitive to NEM, this having been observed for several receptors (Waeber and Chiu, 1999;Laitinen, 2004;Palomäki and Laitinen, 2006). In the present study, pre-treatment with NEM (1 mM) for 30 min clearly decreased the overall labelling observed under basal conditions (Fig.…”

Section: Resultssupporting

confidence: 80%

Neuroanatomical mapping of juvenile rat brain regions with prominent basal signal in [35S]GTPγS autoradiography

Aaltonen¹,

Palomäki²,

Lecklin³

et al. 2008

Journal of Chemical Neuroanatomy

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Section: Resultssupporting

confidence: 80%

Neuroanatomical mapping of juvenile rat brain regions with prominent basal signal in [35S]GTPγS autoradiography

Aaltonen¹,

Palomäki²,

Lecklin³

et al. 2008

Journal of Chemical Neuroanatomy

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“…35 S]GTP␥S autoradiography using S1P has revealed S1P receptor-mediated G-protein activity throughout the brain (Waeber and Chiu, 1999). Of interest in the present study is the apparent overlap in the distribution of S1P and CB 1 receptor-mediated activity in regions including the cerebellum, hippocampus, striatum, and cortex.…”

Section: Discussionmentioning

confidence: 73%

“…CB 2 receptors are located primarily in the immune system, whereas CB 1 receptors are abundant in the CNS and are present in several peripheral organs (Howlett et al, 2002). CB 1 and S1P receptors stimulate G-protein activity in the CNS and are codistributed in many regions (Sim et al, 1995;Waeber and Chiu, 1999). Interest in the S1P receptors has increased because of the therapeutic potential of the immunomodulatory drug FTY720, which targets all of these receptors, except S1P 2 .…”

mentioning

confidence: 99%

Sphingosine and Its Analog, the Immunosuppressant 2-Amino-2-(2-[4-octylphenyl]ethyl)-1,3-propanediol, Interact with the CB₁ Cannabinoid Receptor

Paugh

Cassidy²,

He³

et al. 2006

Mol Pharmacol

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Sphingosine-1-phosphate (S1P) and cannabinoid receptors are G-protein-coupled receptors that mediate the effects of S1P and endocannabinoids, respectively. Cannabinoid receptors also mediate the effects of ⌬ 9 -tetrahydrocannabinol, the primary psychoactive ingredient in marijuana, whereas S1P receptors contribute to the immunosuppressant effects of 2-amino-2-(2-[4-octylphenyl]ethyl)-1,3-propanediol (FTY720). FTY720 is a sphingosine analog that can prevent renal graft rejections and suppress a variety of autoimmune disorders in animal models and clinical trials. We now report that both FTY720 and sphingosine interact with CB 1 but not CB 2 cannabinoid receptors. FTY720 and sphingosine inhibited the binding of the CB 1 -selective antagonist -2) in a concentration-dependent manner, and this antagonist effect was not mimicked by S1P. FTY720 and sphingosine also inhibited activation of extracellular signal-regulated kinases 1 and 2 and Akt by WIN55,212-2 in intact Chinese hamster ovary (CHO) cells expressing CB 1 receptors and attenuated WIN55,212-2-stimulated internalization of a fluorescence-tagged CB 1 receptor in CHO cells. Moreover, both FTY720 and sphingosine produced rightward shifts in the concentration-effect curves of cannabinoid agonists for G-protein activation, indicating that they act as competitive CB 1 antagonists. These results suggest that the CB 1 receptor could be a novel target of FTY720 and that sphingosine could be an endogenous CB 1 antagonist.Sphingosine-1-phosphate (S1P) and cannabinoid receptors belong to the lysolipid family of GPCRs (Toman and Spiegel, 2002). Subtypes for each receptor, S1P 1-5 and CB 1 and CB 2 , have been identified and exhibit unique tissue distributions and functional profiles. There are a number of similarities between S1P and cannabinoid receptors with a notably significant sequence homology of approximately 35% (Toman and Spiegel, 2002). S1P and cannabinoid receptors are actiThese studies were supported by National Institutes of Health grants DA05274, DA14277, and GM43880. Imaging was supported in part by National Institutes of Health grant P30-CA16059.A preliminary version of this work was presented previously in abstract form: Selley DE, Cassidy MP, Milstein S, He HJ, Sim-Selley LJ, Spiegel S, and Paugh SW. ABBREVIATIONS: S1P, sphingosine-1-phosphate; BSA, bovine serum albumin; CNS, central nervous system; ERK, extracellular signal regulated kinase; p-, phosphorylated; GPCR, G-protein-coupled receptor; CHO, Chinese hamster ovary; Pen-Strep, penicillin/streptomycin; FBS, fetal bovine serum; DMEM, Dulbecco's modified Eagle's medium; mCB 1 -CHO, Chinese hamster ovary-K1 cells stably expressing the mouse CB 1 receptor; HEK, human embryonic kidney; hCB 1 -HEK, human embryonic kidney-293 cells stably transfected with the human CB 1 receptor; GFP, green fluorescent protein; rCB 1 -GFP, green fluorescent protein-tagged rat CB 1 receptor; GTP␥S, guanosine

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“…Using S1P-induced 35 S-GTP␥S labeling as a measure of receptor localization, different brain regions have quite variable levels of expression (Sim-Selley et al 2009;Waeber and Chiu 1999). For example, labeling in the cerebellum is high, whereas that in the thalamus is relatively low.…”

Section: Discussionmentioning

confidence: 99%

The Sphingosine 1-Phosphate Receptor, S1PR₁, Plays a Prominent But Not Exclusive Role in Enhancing the Excitability of Sensory Neurons

Xuan

Nicol

2010

Journal of Neurophysiology

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Chi XX, Nicol GD. The sphingosine 1-phosphate receptor, S1PR 1 , plays a prominent but not exclusive role in enhancing the excitability of sensory neurons. J Neurophysiol 104: 2741-2748. First published September 15, 2010 doi:10.1152/jn.00709.2010. Sphingosine 1-phosphate (S1P) through its interaction with a family of G protein-coupled receptors (S1PR) is proving to have a significant impact on the activation of a variety of cell types, most notably those cells mediating the inflammatory response. Previously, we showed that S1P enhanced the excitability of small diameter sensory neurons, and mRNA for S1PR 1-4 was expressed in sensory neurons. These initial findings did not determine which S1PR subtype(s) mediated the increased excitability. Here, we report that exposure to the selective S1PR 1 agonist, SEW2871, produced a significant increase in excitability of some, but not all, sensory neurons. To further examine the role of S1PR 1 , neurons were treated with siRNA targeted to S1PR 1 . siRNA reduced S1PR 1 protein expression by 75% and blocked the sensitization produced by SEW2871, although some neurons remained responsive to subsequent exposure to S1P. Treatment with scramble siRNA did not alter S1PR 1 expression. Recordings from siRNA-and scramble-treated neurons suggested three distinct populations based on their sensitivities to SEW2871 and S1P. Approximately 50% of the neurons exhibited a significant increase in excitability after exposure to SEW2871 and subsequent S1P produced no additional increase; ϳ25% were not affected by SEW2871 but S1P significantly increased excitability; and ϳ25% of the neurons were not sensitized by either SEW2871 or S1P. RT-PCR measurements obtained from single neurons showed that 50% of the small diameter neurons expressed the mRNA for S1PR 1 . These results indicate that S1PR 1 plays a prominent, although not exclusive, role in mediating the enhancement of excitability produced by S1P.

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In Vitro Autoradiographic Visualization of Guanosine‐5’‐O‐(3‐[³⁵S]Thio)triphosphate Binding Stimulated by Sphingosine 1‐Phosphate and Lysophosphatidic Acid

Cited by 38 publications

References 40 publications

Neuroanatomical mapping of juvenile rat brain regions with prominent basal signal in [35S]GTPγS autoradiography

Neuroanatomical mapping of juvenile rat brain regions with prominent basal signal in [35S]GTPγS autoradiography

Sphingosine and Its Analog, the Immunosuppressant 2-Amino-2-(2-[4-octylphenyl]ethyl)-1,3-propanediol, Interact with the CB₁ Cannabinoid Receptor

The Sphingosine 1-Phosphate Receptor, S1PR₁, Plays a Prominent But Not Exclusive Role in Enhancing the Excitability of Sensory Neurons

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In Vitro Autoradiographic Visualization of Guanosine‐5’‐O‐(3‐[35S]Thio)triphosphate Binding Stimulated by Sphingosine 1‐Phosphate and Lysophosphatidic Acid

Cited by 38 publications

References 40 publications

Neuroanatomical mapping of juvenile rat brain regions with prominent basal signal in [35S]GTPγS autoradiography

Neuroanatomical mapping of juvenile rat brain regions with prominent basal signal in [35S]GTPγS autoradiography

Sphingosine and Its Analog, the Immunosuppressant 2-Amino-2-(2-[4-octylphenyl]ethyl)-1,3-propanediol, Interact with the CB1 Cannabinoid Receptor

The Sphingosine 1-Phosphate Receptor, S1PR1, Plays a Prominent But Not Exclusive Role in Enhancing the Excitability of Sensory Neurons

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Product

Resources

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In Vitro Autoradiographic Visualization of Guanosine‐5’‐O‐(3‐[³⁵S]Thio)triphosphate Binding Stimulated by Sphingosine 1‐Phosphate and Lysophosphatidic Acid

Sphingosine and Its Analog, the Immunosuppressant 2-Amino-2-(2-[4-octylphenyl]ethyl)-1,3-propanediol, Interact with the CB₁ Cannabinoid Receptor

The Sphingosine 1-Phosphate Receptor, S1PR₁, Plays a Prominent But Not Exclusive Role in Enhancing the Excitability of Sensory Neurons