In vitro Assessment of Total Bioactive Contents, Antioxidant, Anti-Alzheimer and Antidiabetic Activities of Leaves Extracts and Fractions of Aloe vera

Bendjedid, Samira; Djelloul, Radia; Tadjine, Aicha; Bensouici, Chawki; Boukhari, Abbes

doi:10.12982/cmujns.2020.0031

Cited by 3 publications

(3 citation statements)

References 36 publications

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“…These findings can be explained by the existence of chemicals in the extracts that are responsible for the inhibition of the AChE [ 4 ] and particularly by the ethanolic extract’s flavonoids. Our results agree with those of the investigations conducted by Teyeb et al [ 35 ].…”

Section: Resultsmentioning

confidence: 99%

“…These biomolecules have significant importance in reducing oxidative stress, which could destroy the biological mechanisms in the human body [ 3 ]. In reality, the preference for natural substances is also a consequence of the lack of adverse effects where numerous studies have shown that natural compounds counteract the negative effects of pharmaceutical treatments, implying that these natural molecules have greater medicinal value [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

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Ultrasound-Assisted Extraction, LC–MS/MS Analysis, Anticholinesterase, and Antioxidant Activities of Valuable Natural Metabolites from Astragalus armatus Willd.: In Silico Molecular Docking and In Vitro Enzymatic Studies

Lekmine

Bendjedid²,

Benslama

et al. 2022

Antioxidants

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The Astragalus armatus Willd. plant’s phenolic constituent extraction and identification were optimized using the ultrasound-assisted extraction (UAE) method and the LC–MS/MS analysis, respectively. Additionally, cupric reducing antioxidant capacity (CUPRAC), beta carotene, reducing power, DMSO alcalin, silver nanoparticle (SNP)-based method, phenanthroline, and hydroxyl radical tests were utilized to assess the extract’s antioxidant capacity, while the neuroprotective effect was examined in vitro against acetylcholinesterase enzyme. This study accurately estimated the chemical bonding between the identified phenolic molecules derived from LC–MS/MS and the AChE. The extract was found to contain sixteen phenolic substances, and rosmarinic, protocatechuic, and chlorogenic acids, as well as 4-hydroxybenzoic, hyperoside, and hesperidin, were the most abundant substances in the extract. In all antioxidant experiments, the plant extract demonstrated strong antioxidant activity and a significant inhibitory impact against AChE (40.25 ± 1.41 μg/mL). According to molecular docking affinity to the enzyme AChE, the top-five molecules were found to be luteolin, quercetin, naringenin, rosmarinic acid, and kaempferol. Furthermore, these tested polyphenols satisfy the essential requirements for drug-like characteristics and Lipinski’s rule of five. These results highlight the significance of the A. armatus plant in cosmetics, as food additives, and in the pharmaceutical industry due to its rosmarinic and chlorogenic acid content.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ultrasound-Assisted Extraction, LC–MS/MS Analysis, Anticholinesterase, and Antioxidant Activities of Valuable Natural Metabolites from Astragalus armatus Willd.: In Silico Molecular Docking and In Vitro Enzymatic Studies

Lekmine

Bendjedid²,

Benslama

et al. 2022

Antioxidants

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“…Phenolic compounds are a main class of secondary metabolites in medicinal plants. The beneficial effects of phenolic compounds is attributed to the antioxidant activity that mainly due to the capacity of scavenging free radicals, donating hydrogen atoms, electrons, or chelate metal cations [Bendjedid, et al, 2020]. Gardenia carinata Wallich., belonging to the family Rubiaceae, is native from Malaysia and Indonesia and it has been found in the southern part of Thailand.…”

Section: Introductionmentioning

confidence: 99%

Chemical Constituents Antioxidant and Antibacterial Activities of the Leaves and Flowers from Gardenia carinata Wallich

Warinthip¹,

Liawruangrath²,

Natakankitkul³

et al. 2023

NLSC

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The essential oil of the leaves was isolated by hydrodistillation and the ethanolic extract of the flowers from Gardenia carinata Wallich. were analyzed for the first time using GC and GC-MS. For the leaves essential oil: Seventeen compounds were detected and eleven compounds were identified, corresponding to 71.10% of the total chromatographical oil compounds. The major constituents were phytol (13.80%), 3-hexene-1-ol, benzoate (12.50%), vitispirane (8.00%), hexadecanal (7.90%), β-damascenone (3.90%) and (E,E)-α-farnesene (3.80%). For the flowers extract: Seventeen compounds were identified, constituting 37.5% of the total chromatographical components. The typical compounds were heneicosane (9.70%), ethyl linolenate (5.70%), squalene (5.70%), 4-allylphenol (5.40%), stigmasterol (2.10%), n-docosane (1.60%), lupenone (1.40%), hexadecanoic acid (0.90%) and compesterol (0.70%). The leaves essential oil showed antibacterial activity against both Gram positive S. aureus with the most potent activity at 18.7 µg/disc, and Gram negative E. coli with an activity of 17.6 µg/disc. The oil was not active against Gram negative P. aeruginosa. The antioxidant activity of the leave essential oil and the ethanolic flower extract were evaluated by using the DPPH and ABTS radical scavenging assay. The leave essential oil showed medium antioxidant activity with the IC50 value of 15.24 ± 0.08 and 10.88 ± 0.05 mg/mL. The ethanolic flower extract possessed high antioxidant activity with the IC50 value of 3.68 ± 0.03 and 2.53 ± 0.03 mg/mL. Keywords: Gardenia carinata, GC-MS, Leave essential oil and ethanolic flowers extract, Antibacterial and antioxidant activities.

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Bioactive Analysis of Antibacterial Efficacy and Antioxidant Potential of Aloe barbadensis Miller Leaf Extracts and Exploration of Secondary Metabolites Using GC–MS Profiling

Palaniyappan

Sridhar

Arumugam

et al. 2023

Appl Biochem Biotechnol

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In vitro Assessment of Total Bioactive Contents, Antioxidant, Anti-Alzheimer and Antidiabetic Activities of Leaves Extracts and Fractions of Aloe vera

Cited by 3 publications

References 36 publications

Ultrasound-Assisted Extraction, LC–MS/MS Analysis, Anticholinesterase, and Antioxidant Activities of Valuable Natural Metabolites from Astragalus armatus Willd.: In Silico Molecular Docking and In Vitro Enzymatic Studies

Ultrasound-Assisted Extraction, LC–MS/MS Analysis, Anticholinesterase, and Antioxidant Activities of Valuable Natural Metabolites from Astragalus armatus Willd.: In Silico Molecular Docking and In Vitro Enzymatic Studies

Chemical Constituents Antioxidant and Antibacterial Activities of the Leaves and Flowers from Gardenia carinata Wallich

Bioactive Analysis of Antibacterial Efficacy and Antioxidant Potential of Aloe barbadensis Miller Leaf Extracts and Exploration of Secondary Metabolites Using GC–MS Profiling

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