In Vitro Assessment of the Susceptibility of Planktonic and Attached Cells of Foodborne Pathogens to Bacteriophage P22-Mediated Salmonella Lysates

Ahn, Juhee; Kim, Song‐Rae; Jung, Lae-Seung; Biswas, Debabrata

doi:10.4315/0362-028x.jfp-13-183

Cited by 16 publications

(10 citation statements)

References 34 publications

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“…Bacteria biofilms are highly resistant to antibiotics because of structural rigidity and reduced metabolic activity, which can cause serious persistent and chronic infections (16). Previous study reported that the bacterial cell components, including extracellular DNA, can promote biofilm formation (17). In this study, the phage-induced bacterial lysates, however, did not have a great influence on the induction of biofilm formation by S. aureus in combination.…”

Section: Resultsmentioning

confidence: 54%

Role of phage-antibiotic combination in reducing antibiotic resistance in Staphylococcus aureus

Kim

Ding

et al. 2016

Food Sci Biotechnol

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This study was designed to evaluate the effect of phage-antibiotic synergy in reducing antibiotic resistance. The initial numbers of treated with ciprofloxacin, phages, and combination were significantly reduced by 3.47, 4.62, and 5.75 log CFU/mL, respectively, at the early 12 h of incubation. The combination treatment most effectively inhibited the growth of, showing more than 4 log reduction in 18 h of incubation at 37°C. The significant reduction in biofilm formation by was observed at the combination treatment (3.91 log). Ciprofloxacin-treated cells became resistant to both ciprofloxacin and phage, showing the mutant frequencies of 27% and 25%, respectively, whereas no antibiotic- and phage-resistant cells were observed at the combined treatment of ciprofloxacin and phages. These results provide useful information for reducing the risk of antibiotic resistance in human and food animals.

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Section: Resultsmentioning

confidence: 54%

Role of phage-antibiotic combination in reducing antibiotic resistance in Staphylococcus aureus

Kim

Ding

et al. 2016

Food Sci Biotechnol

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“…Above all, phages only infect bacteria and are not harmful to humans, making them safe for application in the clinic and food products ( 24 ). A recent study found that phages have high efficiency in reducing and controlling bacterial biofilms on various surfaces, such as those produced by Pseudomanas aeruginosa, Salmonella, E. coli , and Listeria monocytogenes ( 25 – 28 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Lytic Phage Flora With a Broad Host Range Against Multidrug-Resistant Escherichia coli and Evaluation of Its Efficacy Against E. coli Biofilm Formation

Jiang

et al. 2022

Front. Vet. Sci.

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Escherichia coli is a gram-negative bacterium that is distributed widely throughout the world; it is mainly found in contaminated food, the poultry industry, and animal feces. The emergence of antibiotic-resistant E. coli poses a threat to human and animal health, which has led to renewed interest in phage-based therapy. E. coli biofilm control and prevention are of great importance. In this study, the isolated phages Flora and KM18 were found to belong to the family Myoviridae; the optimal preservation buffer was pH = 6~7, and the phage genome sizes were 168,909 (Flora) and 168,903 (KM18) bp. Phage Flora had a broader lytic spectrum than KM18. Phage Flora had a better antibiofilm effect than kanamycin sulfate in high-concentration E. coli cultures. A combination of the phage Flora and kanamycin sulfate showed better antibiofilm effects than Flora or kanamycin sulfate alone in low-concentration E. coli cultures. These characteristics can serve as a guideline for the selection of effective candidates for phage therapy, in this case antibiotic-resistant E. coli control in the poultry industry.

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“…In addition, phages only infect bacteria and are not harmful to humans, making them a safe application in clinical and food products [25]. A recent study found that phages are highly effective at reducing and controlling bacterial biofilms on various surfaces formed by species such as Escherichia coli, Salmonella sp., Pseudomonas aeruginosa, and Listeria monocytogenes [26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of a Lytic Staphylococcus aureus Phage WV against Staphylococcus aureus Biofilm

Jiang

et al. 2021

Intervirology

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Background: Staphylococcus aureus is a Gram-positive, pathogenic bacterium that causes a wide range of symptoms in humans and can form biofilm, which is a multicellular community of microorganisms that attaches to nonbiological and biological surfaces. Methods: Here, we aimed to isolate and characterize an S. aureus phage and examine the bactericidal activity alone and in conjunction with streptomycin treatment. Results: We isolated a virulent phage, WV, from a slaughterhouse in Jiangsu, China. This strain belonged to the family Myoviridae and presented a genome size of 141,342 bp. The optimal pH of the preservation buffer was 6–7, optimal growth temperature was 37°C, and optimal multiplicity of infection was 0.01. Phage WV can sterilize most clinical strains of S. aureus that had been isolated from clinical patients in the First People’s Hospital of the Yunnan Province. Against low-concentration S. aureus culture, streptomycin demonstrated a greater antibiofilm effect than that of phage WV. By contrast, in high-concentration S. aureus culture, phage WV demonstrated greater antibiofilm effect than that of streptomycin. The use of phage WV and streptomycin together had a substantially greater overall antibiofilm effect than that achieved using either component alone. Conclusion: This study provides strong evidence for the effectiveness of phage application for the reduction of S. aureus biofilm growth and suggests that phages can be considered as a viable alternative to antibiotics in clinical settings.

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In Vitro Assessment of the Susceptibility of Planktonic and Attached Cells of Foodborne Pathogens to Bacteriophage P22-Mediated Salmonella Lysates

Cited by 16 publications

References 34 publications

Role of phage-antibiotic combination in reducing antibiotic resistance in Staphylococcus aureus

Role of phage-antibiotic combination in reducing antibiotic resistance in Staphylococcus aureus

Characterization of the Lytic Phage Flora With a Broad Host Range Against Multidrug-Resistant Escherichia coli and Evaluation of Its Efficacy Against E. coli Biofilm Formation

Isolation and Characterization of a Lytic <b><i>Staphylococcus aureus</i></b> Phage WV against <b><i>Staphylococcus aureus</i></b> Biofilm

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