In vitro assessment of hepatoprotective agents against damage induced by acetaminophen and CCl4

Abstract

BackgroundIn vitro bioassays are important in the evaluation of plants with possible hepatoprotective effects. The aims of this study were to evaluate the pretreatment of HepG2 cells with hepatoprotective agents against the damage induced by carbon tetrachloride (CCl4) and paracetamol (APAP).MethodsAntioxidative activity was measured using an assay to measure 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging. The in vitro hepatotoxicity of CCl4 and APAP, and the cytotoxic and hepatoprotective properties of silymarin (SLM), silybinin (SLB), and silyphos (SLP) were evaluated by measuring cell viability; activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH); total antioxidant capacity (TAOxC); and reduced glutathione (GSH), superoxide dismutase (SOD), and lipid peroxidation (malondialdehyde (MDA) levels).ResultsOnly SLB and SLM showed strong antioxidative activity in the DPPH assay (39.71 ± 0.85 μg/mL and 14.14 ± 0.65 μg/mL, respectively). CCl4 induced time- and concentration-dependent changes. CCl4 had significant effects on cell viability, enzyme activities, lipid peroxidation, TAOxC, and SOD and GSH levels. These differences remained significant up to an exposure time of 3 h. APAP induced a variety of dose- and time-dependent responses up to 72 h of exposure. SLM, SLB, and SLP were not cytotoxic. Only SLB at a concentration of 100 μg/mL or 150 μg/mL significantly decreased the enzyme activities and MDA level, and prevented depletion of total antioxidants compared with CCl4.ConclusionsCCl4 was more consistent than APAP in inducing cell injury. Only SLB provided hepatoprotection. AST, LDH, and MDA levels were good markers of liver damage.

Hepatoprotective Effect of Steroidal Glycosides From Dioscorea villosa on Hydrogen Peroxide-Induced Hepatotoxicity in HepG2 Cells

“…Therefore, this study was aimed to investigate the hepatoprotective effect of the constituents of D. villosa against H 2 O 2 -induced cytotoxicity and oxidative stress in HepG2 cell line. HepG2 cell line is well reported to be involved in the metabolism ( Kim et al, 2011 ) and have been proven to be a good model system for cytotoxicity assessment and cyto-protective potential of various compounds against the toxic substances ( González et al, 2017 ).…”

Comparison of microRNA expressions for the identification of chemical hazards in in vivo and in vitro hepatic injury models

“…Our studies demonstrated that ALT, AST and LDH activity could be variable in in vitro models. Enzyme activity in biofluids can be affected by the dose and duration of exposure to toxicants (González et al, ; Kia et al, ). Nevertheless, most miRNAs except for miR‐122 and miR‐193a were significantly elevated in the supernatants of HepG2 cell cultures consistent with cell viability (Table ).…”

Dietary exposure of deoxynivalenol affected cytochrome P450 and growth related-gene expression via DNA methylation in piglet liver

“…Herein, DON at 1 mg/kg elevated the ALT and GGT levels, suggesting that DON may destroy the liver cell membrane, leading toleakage of enzymes from injured hepatocytes [54,55]. However, compared with the 1 mg/kg DON group, DON at 3 mg/kg decreased serum levels of ALT, AST and LDH, especially AST and LDH, suggesting that 3 mg/kg DON may lead to massive necrosis of liver cells or acute hepatitis, basically resulting in depletion of transaminase in the liver tissue [56,57]. In addition, DON signi cantly increased the level of serum GLO, suggesting that DON may cause an in ammatory response and immune system disorders in piglet liver [58].…”