In vitro assessment of dermatological activity potential of Achillea clypeolata Sm. in H2O2-treated human dermal fibroblasts

Uncovering chemical profiles, biological potentials, and protection effect against ECM destruction in H2O2‐treated HDF cells of the extracts of Stachys tundjeliensis

“…To test the inhibition activity of ethanol, ethanol/water, and water extracts obtained from S. tundjeliensis on collagenase, elastase, and hyaluronidase skin enzymes, the time-dependent inhibition activity of the extracts at 1 mg/mL concentration was measured. [67][68][69] In Figure 9a, time-dependent anti-collagenase activity results of EtOH extract of S. tundjeliensis are presented. A total of 250 µg/mL of EGCG was used as a positive control.…”

“…and demonstrate the effect of S. tundjeliensis on MMPs that may change accordingly. In this context, the ROS mechanism was activated by applying 75 µmol/L of H 2 O 2 to HDF cells for 2 h. [46,67] On the other hand, 250 µg/mL of EGCG was applied to H 2 O 2 -induced HDF cells for 48 h as a positive control. [46,67] In the gel image showing MMP-2 and MMP-9 activity in Figure 10a, active MMPs broke down their substrates in their respective areas and formed white lytic bands.…”

“…[46,67,94] To trigger the ROS-driven molecular mechanism in HDF cells, the chemical component hydrogen peroxide (H 2 O 2 ), which we have previously used and whose concentration we determined in the same cell line, was used. [46,67] The ROS mechanism was activated by applying 75 µmol/L of H 2 O 2 to HDF cells for 2 h. On the other hand, commercially available 250 µg/mL of EGCG, which is known to have a ROS scavenging effect, was applied to H 2 O 2 -induced HDF cells for 48 h and the cells in this group were determined as a positive control. [46,67] HDF cells that were not treated with H 2 O 2 were called control HDF groups.…”

“…[46,67] The ROS mechanism was activated by applying 75 µmol/L of H 2 O 2 to HDF cells for 2 h. On the other hand, commercially available 250 µg/mL of EGCG, which is known to have a ROS scavenging effect, was applied to H 2 O 2 -induced HDF cells for 48 h and the cells in this group were determined as a positive control. [46,67] HDF cells that were not treated with H 2 O 2 were called control HDF groups.…”

“…At each determined time point, the WST-1 reagent was diluted 1:10 and applied to the cells for 1 h, and then quantitative analysis was performed at 570 nm to determine its effects on cell proliferation colourimetrically. [46,67,[92][93][94] 4.10 Detection of cellular ROS level with 2′,7′dichlorofluorescein diacetate (DCFDA)…”