In-vitro Assessment of Carbendazim and Copper oxychloride cytotoxicity on HaCaT and HepG2 human cell lines

Abstract

The objective of present study was to evaluate the cytotoxic effect of two fungicides i.e. carbendazim and copper oxychloride on human cell lines HaCaT [keratinocyte] and HepG2 [hepatoma cell line]. The cytotoxic study was done by following the standard MTT [3-[4,5-dimethylthiazol-2-yl]-2,5-diphehyltetrazolium bromide] assay along with standard drug. The MTT assay results showed that these two fungicides showed concentration dependent cytotoxicity, upon incubation for 24 hours with concentration ranging 20-450 µg/ml and IC50 values were determined. The cell viability of HaCaT cells decreased from 100% to 35.86% with increase in carbendazim concentration 20-450 µg/ml. Whereas HepG2 cells were sensitive towards Carbendazim treatment with the decrease in viability up to 30.98% at 450 μg/ml. Copper oxychloride was more lethal, causing total cell death of HaCaT cells at 350 μg/ml, and HepG2 at 450 μg/ml and the present work correlate this toxicity caused by these two fungicides on human skin and liver. The study u nveils the cytotoxic effects of these fungicides on the skin as well as on the liver indicating the long term exposure to these compounds may lead to deleterious effects. Further study is needed on to identify the mechanism and pathway involved in the cytotoxic activity of these fungicides on HaCaT and HepG2 cell lines.