In vitro assessment of artificial saliva formulations on initial enamel erosion remineralization

Ionta, Franciny Querobim; Mendonça, Fernanda Lyrio; Oliveira, Gabriela Cristina de; Alencar, Catarina Ribeiro Barros de; Honório, Heitor Marques; Magalhães, Ana Carolina; Ríos, Daniela

doi:10.1016/j.jdent.2013.11.009

Cited by 82 publications

(85 citation statements)

References 34 publications

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“…In contrast to the results of the present study, a recent study by Ionta et al [2014] found differences in the rehardening potential of various artificial saliva formulations and water. This study did not use a de-and remineralization protocol, but focused on the remineralization of erosively softened enamel (citric acid, pH 2.5, 15 s) after 2 h of storage time.…”

Section: Discussioncontrasting

confidence: 99%

Artificial Saliva Formulations versus Human Saliva Pretreatment in Dental Erosion Experiments

et al. 2016

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The aim of this study was to evaluate the erosion-preventive effect of different artificial saliva formulations and human saliva in vitro compared to human saliva in situ. In the in vitro experiment, bovine enamel and dentin specimens were stored in artificial saliva (4 different formulations, each n = 20), deionized water (n = 20) or human saliva (n = 6 enamel and dentin specimens/volunteer) for 120 min. In the in situ experiment, each of the 6 enamel and dentin specimens was worn intraorally by 10 volunteers for 120 min. The specimens were then eroded (HCl, pH 2.6, 60 s). Half of the specimens were subjected to microhardness analysis (enamel) and the determination of calcium release into the acid (enamel and dentin), while the other half were again placed in the respective medium or worn intraorally, respectively, for 120 min before a second erosion was performed. Knoop microhardness of enamel and the calcium release of enamel and dentin into the acid were again determined. Statistical analysis was conducted by two-way repeated-measures ANOVA or two-way ANOVA (α = 0.05). Enamel microhardness was not significantly different between all test groups after the first and the second erosive challenge, respectively. Enamel calcium loss was significantly lower in situ compared to the in vitro experiment, where there was no significant difference between all test groups. Dentin calcium loss was significantly lower than deionized water only after the first and than all except one artificial saliva after the second erosion. Under the conditions of this experiment, the use of artificial saliva formulations and human saliva in vitro does not reflect the intraoral situation in dental erosion experiments adequately.

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Section: Discussioncontrasting

confidence: 99%

Artificial Saliva Formulations versus Human Saliva Pretreatment in Dental Erosion Experiments

et al. 2016

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“…In the present study, the concentration of porcine mucin in the artificial saliva (2.7 g/L) was according to the formulation of Klimek et al [1982]. A similar amount was also used in previous studies [Cheaib and Lussi, 2011;Ionta et al, 2014]. This concentration is higher than the physiological concentration of mucin in vivo [Carpenter et al, 2014]; however, the rehardening effect did not differ from that one without mucin, in accordance with previous studies [Ionta et al, 2014].…”

Section: Discussionsupporting

confidence: 80%

“…SHi, initial superficial hardness; SHd, hardness after erosive demineralization; SHf, final hardness after the effect of types of saliva or water; %SHR, hardness recovery = [(SHf -SHd)/(SHi -SHd)] × 100. There are some conflicting results regarding the effect of mucin on enamel remineralization [Kapsimalis et al, 1966;Vissink et al, 1985;Nieuw Amerongen et al, 1987;Kielbassa et al, 2001Kielbassa et al, , 2005Hara et al, 2008;Ionta et al, 2014]. A high concentration of this glycoprotein increases the viscosity of the artificial saliva compared to human saliva, which may decrease the mineral diffusion [Vissink et al, 1985;Hara et al, 2008].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Mucin in Artificial Saliva on Erosive Rehardening and Demineralization

et al. 2017

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The effect of mucin in artificial saliva on rehardening (RE-experiment) and inhibition (DE-experiment) of erosion was evaluated. The treatment groups were: artificial saliva with mucin, artificial saliva without mucin, human saliva, and water. For the RE-experiment, after immersion of enamel blocks in citric acid (4 min), hardness was measured and blocks were subjected to treatment for 2 h. For the DE-experiment, sound blocks were subjected to treatment for 2 h and immersed in citric acid (4 min). Percentages of hardness recovery (RE) and loss (DE) were analyzed (ANOVA/Tukey's test). The salivas promoted similar rehardening, but only the saliva with mucin was similar to human saliva with regard to enamel protection against erosion.

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“…Other popular physiological solutions comprise Hanks' balanced salt solution (HBSS), which contains almost similar inorganic salts and glucose (Hanks and Wallace 1949;Shibata et al 2004;Marques et al 2003a;Mareci et al 2010), Eagle's minimum essential medium (MEM) and its variation Dulbecco's modified Eagle's medium (DMEM), which contain numerous bioorganic (alanine, aspartic acid, glycine, biotin, vitamin C, folic acid, riboflavin) and inorganic (CaCl 2 , KCl, NaCl, NaH 2 PO 4 ) components (Meuleman et al 2006;Touny et al 2011;Coelho et al 2000;Mandel and Tas 2010;Rohanová et al 2014), phosphate-buffered saline (PBS) that contains only inorganic (CaCl 2 , MgCl 2 , KCl, KH 2 PO 4 , NaCl, NaH 2 PO 4 ) components (Gao et al 2006;Lichtenauer et al 2011). Furthermore, artificial saliva Ionta et al 2014;Okulus et al 2014), synthetic urine (Assimos 2013Dbira et al 2015) and simulated milk ultrafiltrate (SMUF) (Jenness and Koops 1962;Spanos et al 2007;Gao et al 2010a, b) solutions are available. They contain both bioorganic (e.g., xanthan gum or sodium carboxymethylcellulose, sorbitol, etc.)…”

Section: Biomimetic Crystallization Of Capomentioning

confidence: 99%

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

Dorozhkin¹

2017

Morphologie

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In vitro assessment of artificial saliva formulations on initial enamel erosion remineralization

Cited by 82 publications

References 34 publications

Artificial Saliva Formulations versus Human Saliva Pretreatment in Dental Erosion Experiments

Artificial Saliva Formulations versus Human Saliva Pretreatment in Dental Erosion Experiments

The Effect of Mucin in Artificial Saliva on Erosive Rehardening and Demineralization

Calcium orthophosphates (CaPO 4 ): Occurrence and properties

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