In vitro assessment of a novel, hypothermically stored amniotic membrane for use in a chronic wound environment

McQuilling, John P.; Vines, Jeremy B.; Mowry, Katie C.

doi:10.1111/iwj.12748

Cited by 40 publications

(55 citation statements)

References 81 publications

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“…Proteomic analysis of dACM was conducted as described in detail elsewhere . Briefly, a custom quantitative multiplex enzyme‐linked immunosorbent assay (ELISA) proteomics microarray (Raybiotech Inc, Norcross, Georgia) was used to quantify growth factors and cytokines within dACM.…”

Section: Methodsmentioning

confidence: 99%

“…Cell proliferation experiments were conducted as previously described in detail . Briefly, fibroblasts and keratinocytes were cultured with 50%, 25%, and 10% (vol/vol) dACM CM for 14 days (fibroblasts) or 10 days (keratinocytes) under standard conditions.…”

Section: Methodsmentioning

confidence: 99%

“…Clinical studies of placental membranes have demonstrated improved outcomes in a variety of wound types, including: acute wounds, DFUs, and venous leg ulcers . Placental tissues are processed in various ways, including: cryopreservation, hypothermic storage, and dehydration . Composition and processing alters the proteomic composition and may consequently affect how the grafts function …”

Section: Introductionmentioning

confidence: 99%

“…There are several beneficial properties that placental membranes natively possess that may support wound healing. In addition to acting as a protective physical covering, placental membranes have been reported to contain a wide variety of growth factors and cytokines, which have been shown to promote beneficial cellular processes in vitro …”

Section: Introductionmentioning

confidence: 99%

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Characterisation of dehydrated amnion chorion membranes and evaluation of fibroblast and keratinocyte responses in vitro

McQuilling

Kammer

Kimmerling

et al. 2019

International Wound Journal

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The purpose of this study is to characterise the composition of a dehydrated amnion and chorion graft and investigate how factors released from this graft interact with cells important to the wound microenvironment using in vitro models. Characterisation was completed by proteomic analysis of growth factors and cytokines, evaluation of matrix components and protease inhibition, immunohistochemistry, and in vitro release of key growth factors and cytokines. To evaluate the effect of released factors on cells found within the microenvironment, in vitro assays including: cell proliferation, migration, gene expression, protein production, and intracellular pathway activation were used; additionally, responses of fibroblasts in the context of inflammation were measured. We found that released factors from dehydrated amnion/chorion membranes (dACM) stimulated cell proliferation, migration, and altered gene and protein expression profiles of cells important for wound repair in vitro. When cells were cultured in the presence of pro‐inflammatory cytokines, the addition of releasate from dACM resulted in an altered production of cytokines, including a reduction of pro‐inflammatory regulated on activation, normal T cell expressed and secreted (RANTES). In sum, the results presented here characterise the components of dACM, and in vitro studies were used to evaluate interactions of dACM with cell types important in wound healing.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characterisation of dehydrated amnion chorion membranes and evaluation of fibroblast and keratinocyte responses in vitro

McQuilling

Kammer

Kimmerling

et al. 2019

International Wound Journal

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show abstract

“…Placental‐derived tissues have been shown to support repair of several tissue types, including bone, tendon, and skin . They also have known angiogenic properties, often attributed to the presence of angiogenic growth factors within the matrix . Although numerous studies have shown angiogenic properties of placental tissues in a variety of models, relatively little work has been done to characterize the potential mechanisms through which these tissues may promote angiogenesis.…”

Section: Introductionmentioning

confidence: 99%

A mechanistic evaluation of the angiogenic properties of a dehydrated amnion chorion membrane in vitro and in vivo

McQuilling

Burnette

Kimmerling

et al. 2019

Wound Repair Regeneration

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Angiogenesis is essential for the successful repair of tissues; however, in many chronic conditions, angiogenesis is inhibited. Placental tissues have been shown to illicit an angiogenic response both in vitro and in vivo, and the angiogenic properties of these tissues likely contribute to observed clinical outcomes. Although there is some work describing the angiogenic effects of these tissues, comparatively little has been done to determine the possible mechanisms responsible for this effect. The purpose of this study was to conduct a thorough evaluation of a commercially available dehydrated amnion chorion membrane to better understand how these tissues may promote angiogenesis. The proteomic content of this tissue was evaluated using a high throughput proteomic microarray, and then the effects of these grafts were evaluated in vivo using subcutaneous gelfoam sponge implants containing conditioned media (CM) from the graft. Human microvascular endothelial cells were then used to determine how released factors effect migration, proliferation, gene expression, and protein production in vitro. Finally, to elucidate potential signaling‐pathways through which tissue‐derived factors act to induce pro‐angiogenetic phenotypes in endothelial cells in vitro, we performed a global analysis of both serine/threonine and tyrosine kinase activity. Kinomic and proteomic data were then combined to generate protein–protein interaction networks that enabled the identification of multiple growth factors and cytokines with both pro‐ and anti‐angiogenetic properties. In vivo, the addition of CM resulted in increased CD31 and αSMA staining and increases in pro‐angiogenic gene expression. In vitro, CM resulted in significant increases in endothelial proliferation, migration, and the expression of granulocyte‐macrophage colony‐stimulating factor, hepatocyte growth factor, and transforming growth factor beta‐3. Integrated kinomic analysis implicated ERK1/2 signaling as the primary pathway activated following culture of endothelial cells with dehydrated amnion/chorion membrane (dACM) CM. In conclusion, dACM grafts triggered pro‐angiogenic responses both in vitro and in vivo that are likely at least partially mediated by ERK1/2 signaling.

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Biological characterization of dehydrated amniotic membrane allograft: Mechanisms of action and implications for wound care

et al. 2020

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There is a growing clinical demand in the wound care market to treat chronic wounds such as diabetic foot ulcers. Advanced cell and tissue-based products (CTPs) are often used to address challenging chronic wounds where healing has stalled. These products contain active biologics such as growth factors and cytokines as well as structural components that support and stimulate cell growth and assist in tissue regeneration. This study addresses the in vitro biologic effects of a clinically available dehydrated amniotic membrane allograft (DAMA). The broad mechanism of action results from DAMA's biologic composition that leads to stimulation of cell migration cell proliferation, and reduction of pro-inflammatory cytokines. Results show that DAMA possesses growth factors and cytokines such as EGF, FGF, PDGFs, VEGF, TGF-β, IL-8, and TIMPs 1 and 2. Furthermore, in vitro experiments demonstrate that DAMA stimulates cell proliferation, cell migration, secretion of collagen type I, and the reduction of pro-inflammatory cytokines IL-1β, IL-6, and TNF-α. This study findings are consistent with the clinical benefits previously published for DAMA and other CTPs in chronic wounds suggesting that the introduction of DAMA to nonhealing, complex wounds helps to improve the wound milieu by providing essential structural components, cytokines, and growth factors to create an appropriate environment for wound healing.

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In vitro assessment of a novel, hypothermically stored amniotic membrane for use in a chronic wound environment

Cited by 40 publications

References 81 publications

Characterisation of dehydrated amnion chorion membranes and evaluation of fibroblast and keratinocyte responses in vitro

Characterisation of dehydrated amnion chorion membranes and evaluation of fibroblast and keratinocyte responses in vitro

A mechanistic evaluation of the angiogenic properties of a dehydrated amnion chorion membrane in vitro and in vivo

Biological characterization of dehydrated amniotic membrane allograft: Mechanisms of action and implications for wound care

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