In vitro assessment for cytotoxicity screening of new antimalarial candidates

Mechanistic insight into the synergistic antimicrobial potential of Fagonia indica Burm.f. extracts with cefixime

“…The nauplii were treated with various concentrations of plant extract for 24 h. The number of motile nauplii was counted to assess the extract’s effectiveness. It is simple to use, reasonably priced and requires very little test material ( Espíndola et al, 2022 ). The assay was performed at a MIC value of 300 μg/ml (all extracts) and FICI value of 150 + 100 μg/ml (extract + cefixime) with serial dilutions.…”

Synthesis and biological evaluation of novel hybrid compounds bearing pyrazine and 1,2,4-triazole analogues as potent antitubercular agents

“…These cellular entities were cultivated in 96-well flat-bottomed microtiter plates utilizing DMEM supplemented with 10% heat-inactivated fetal calf serum (FBS) and 1% antibiotic–antimycotic 100× solution. 28 Subsequently, they were housed in an incubator maintained at a temperature of 37 °C, 95% humidity, and 5% CO 2 concentration for 24 hours. The cellular entities were then exposed to these distinct drug concentrations and subjected to an additional incubation period of 72 hours.…”

Tendências Biotecnológicas Sustentáveis para Fins de Saúde Única

“…A integração dos testes de citotoxicidade e a avaliação do potencial terapêutico desempenham um papel essencial na aplicação dos metabólitos para o avanço das novas terapias; esses procedimentos fornecem informações experienciais para garantir a segurança e eficácia de novos compostos, contribuindo para orientar as decisões na pesquisa farmacêutica e biomédica (Espíndola et al, 2022;Garcia-Hernando et al, 2020). À medida que ocorre a progressão no desenvolvimento de terapias mais avançadas e personalizadas, a aplicação dessas técnicas continua sendo fundamental para impulsionar a inovação nas áreas das ciências da saúde.…”