In Vitro Assembly of Virus-Like Particles and Their Applications

Le, Dang Thi Thanh; Müller, Kristian M.

doi:10.3390/life11040334

Cited by 54 publications

(50 citation statements)

References 150 publications

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“…The capsid self-assembly of VLPs is a spontaneous natural process by which highly ordered structures arise from the interactions between protein monomers, also known as building blocks. The self-association between building blocks is facilitated by a thermodynamic equilibrium based on van der Waals, hydrogen bonding, hydrophobic, and electrostatic interactions during the nucleation and growth phases [ 40 , 41 ]. As a result, VLPs can adopt different structural arrangements, such as helical, icosahedral, spherical, or complex shapes [ 42 , 43 ] ( Figure 1 ).…”

Section: Key Concepts About Virus-like Particles (Vlps)mentioning

confidence: 99%

Virus-like Particles: Fundamentals and Biomedical Applications

Mejía-Méndez

Vázquez-Duhalt²,

Hernández

et al. 2022

IJMS

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Nanotechnology is a fast-evolving field focused on fabricating nanoscale objects for industrial, cosmetic, and therapeutic applications. Virus-like particles (VLPs) are self-assembled nanoparticles whose intrinsic properties, such as heterogeneity, and highly ordered structural organization are exploited to prepare vaccines; imaging agents; construct nanobioreactors; cancer treatment approaches; or deliver drugs, genes, and enzymes. However, depending upon the intrinsic features of the native virus from which they are produced, the therapeutic performance of VLPs can vary. This review compiles the recent scientific literature about the fundamentals of VLPs with biomedical applications. We consulted different databases to present a general scenario about viruses and how VLPs are produced in eukaryotic and prokaryotic cell lines to entrap therapeutic cargo. Moreover, the structural classification, morphology, and methods to functionalize the surface of VLPs are discussed. Finally, different characterization techniques required to examine the size, charge, aggregation, and composition of VLPs are described.

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Section: Key Concepts About Virus-like Particles (Vlps)mentioning

confidence: 99%

Virus-like Particles: Fundamentals and Biomedical Applications

Mejía-Méndez

Vázquez-Duhalt²,

Hernández

et al. 2022

IJMS

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“…The characteristics, advantages, limitations, and yield ranges of the production systems are compared in Table 1 [ 41 , 45 ]. The correct folding and assembly of VLPs is a complex process, which is highly dependent on the structure of viral proteins, the expression system, and physical parameters, such as, for instance, pH, ionic strength, and temperature [ 39 , 46 , 47 ]. Three methods of cell culture are commonly used for VLPs production, including batch, fed-batch, and continuous cultivation.…”

Section: Various Systems Used To Produce Vlpsmentioning

confidence: 99%

Can Virus-like Particles Be Used as Synergistic Agent in Pest Management?

Deshayes

Gosselin-Grenet

Ogliastro

et al. 2022

Viruses

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Among novel strategies proposed in pest management, synergistic agents are used to improve insecticide efficacy through an elevation of intracellular calcium concentration that activates the calcium-dependent intracellular pathway. This leads to a changed target site conformation and to increased sensitivity to insecticides while reducing their concentrations. Because virus-like particles (VLPs) increase the intracellular calcium concentration, they can be used as a synergistic agent to synergize the effect of insecticides. VLPs are self-assembled viral protein complexes, and by contrast to entomopathogen viruses, they are devoid of genetic material, which makes them non-infectious and safer than viruses. Although VLPs are well-known to be used in human health, we propose in this study the development of a promising strategy based on the use of VLPs as synergistic agents in pest management. This will lead to increased insecticides efficacy while reducing their concentrations.

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“…The discrete "inner and outer" surfaces of VLPs provide a unique platform for concealing drug payloads within a VLP while modifying the outer surface for targeting. Therefore, VLPs offer a unique opportunity to combine benefits of the multiple surfaces and supramolecular self-assembly of liposomes with the polyvalency and robustness of polymers, while also coming in a variety of shapes and sizes that provide a unique versatility over most other materials [41]. They are so diverse that their innate substructures have therapeutic applications for a multitude of cancer treatment such as chemotherapy, phototherapy [42], immunotherapy [43] and combinations with imaging.…”

Section: Figurementioning

confidence: 99%

Virus Like Particles: A Self-Assembled Toolbox for Cancer Therapy

Shahrivarkevishahia

Hagge

Brohlin

et al. 2022

Preprint

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Nanoparticle-based therapeutics have been applied in a broad range of clinical and pre-clinical applications from diagnosis to treatment for cancer. A wide range of synthetic and naturally occurring materials such as polymers, metal oxides, silicate, liposomes, and carbon nanotubes have been developed to overcome key barriers in small molecule therapeutics including intracellular trafficking, cell/tissue targeting, poor biodistribution, and low efficiency. Virus like particles (VLPs)—engineered and non-infectious self-assembling systems based on viral nanostructures—are new approach toward overcoming these limitations, as they are a protein-based nanomaterial that closely mimics the highly symmetrical and polyvalent conformation of viruses while lacking the viral genomes. Their innate biocompatibility, biodegradability, monodispersity, mild immunogenicity, and safety combined with the capacity to chemically modify the interior and exterior surfaces of these systems offer scientists a highly customizable tool to design and engineer multi-component therapeutic agents. In this review, we discuss how these systems have been used in a wide array of cancer treatments including phototherapy, immunotherapy, gene therapy, and chemotherapy.

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In Vitro Assembly of Virus-Like Particles and Their Applications

Cited by 54 publications

References 150 publications

Virus-like Particles: Fundamentals and Biomedical Applications

Virus-like Particles: Fundamentals and Biomedical Applications

Can Virus-like Particles Be Used as Synergistic Agent in Pest Management?

Virus Like Particles: A Self-Assembled Toolbox for Cancer Therapy

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