In vitro assays for characterization of distinct multiple catalytic activities of thyroid peroxidase using LC-MS/MS

Abstract

Graphical abstract

Development and experimental validation of 3D QSAR models for the screening of thyroid peroxidase inhibitors using integrated methods of computational chemistry

“…The well optimized in vitro assay by Tater et al was used to evaluate the TPO inhibitory potential of molecules in the external dataset and the L-Tyr-to-Methyl methionine conversion was observed by LC-MS/MS [ 13 ]. Half maximal inhibitory concentration (IC 50 ), a numerical indicator of test chemical inhibitor potency, was calculated using GraphPad Prism software for N = 3 and reported as a measure of variability for the IC 50 values as tabulated in Table 1 .…”

“…Currently multiple in vitro assays like high-throughput screening (HTPS) assays to screen TPO inhibitors, the catalyst for TH synthesis, LC-MS based method for the characterization of distinct multiple catalytic activities of TPO are reported [ 12 , 13 ]. However, for the screening of large libraries of novel molecules, it is necessary to synthesize and purify the molecules.…”

Dual Signals Electrochemical Biosensor for Point‐of‐Care Testing of Amino Acids Enantiomers

“…Not all chiral enantiomers can play an active role in organisms. Only L‐AA is an essential amino acid that constitutes the protein of the human body [16–18]. Taking tyrosine (Tyr) as an example, L‐Tyr participates in physiological activities of the human body and is accepted as an essential precursor for the synthesis of thyroxine [18–20], epinephrine [21–23], dopamine and other substances [24–26].…”

“…Only L‐AA is an essential amino acid that constitutes the protein of the human body [16–18]. Taking tyrosine (Tyr) as an example, L‐Tyr participates in physiological activities of the human body and is accepted as an essential precursor for the synthesis of thyroxine [18–20], epinephrine [21–23], dopamine and other substances [24–26]. The lack of L‐Tyr will lead to the decline of thyroid function and the generation of various diseases [21, 27–28].…”