In Vitro Assay of Ethanolic Heat Reflux Extract of<i>Nicotiana tabacum</i>L. var Virginia Against Nosocomial Bacteria Pathogen

Pramono, Andri; Fauzantoro, Ahmad; Hidayati, Irma Rizki; Hygea, Arina; Puspita, O. S.; Muktamiroh, Hikmah; Simanjuntak, Kristina; Gozan, Misri

doi:10.1088/1742-6596/970/1/012021

Cited by 10 publications

(5 citation statements)

References 3 publications

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“…The in vitro antibacterial activity of developed hydrogels was determined by Kirby-Bauer diffusion [ 25 ]. P. aeruginosa GIM1.551, S. aureus ATCC 25923, P. suis PM-29, and S. epidermidis LY8 were cultured for 12 h at 37°C in Tryptic Soy Broth (TSB; Qingdao Hope Bio-Technology Co., Ltd., China) liquid medium, the concentration of the bacterial suspension was further adjusted to 0.5 McFarland turbidity, and then 200 μL of bacterial suspension was uniformly transferred to the TSB solid medium.…”

Section: Methodsmentioning

confidence: 99%

Sustained release of alginate hydrogel containing antimicrobial peptide Chol-37(F34-R) in vitro and its effect on wound healing in murine model of Pseudomonas aeruginosa infection

Shi,

Dong,

Chen

et al. 2023

J Vet Sci

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Background Antibiotic resistance is a significant public health concern around the globe. Antimicrobial peptides exhibit broad-spectrum and efficient antibacterial activity with an added advantage of low drug resistance. The higher water content and 3D network structure of the hydrogels are beneficial for maintaining antimicrobial peptide activity and help to prevent degradation. The antimicrobial peptide released from hydrogels also hasten the local wound healing by promoting epithelial tissue regeneration and granulation tissue formation. Objective This study aimed at developing sodium alginate based hydrogel loaded with a novel antimicrobial peptide Chol-37(F34-R) and to investigate the characteristics in vitro and in vivo as an alternative antibacterial wound dressing to treat infectious wounds. Methods Hydrogels were developed and optimized by varying the concentrations of crosslinkers and subjected to various characterization tests like cross-sectional morphology, swelling index, percent water contents, water retention ratio, drug release and antibacterial activity in vitro , and Pseudomonas aeruginosa infected wound mice model in vivo . Results The results indicated that the hydrogel C proved superior in terms of cross-sectional morphology having uniformly sized interconnected pores, a good swelling index, with the capacity to retain a higher quantity of water. Furthermore, the optimized hydrogel has been found to exert a significant antimicrobial activity against bacteria and was also found to prevent bacterial infiltration into the wound site due to forming an impermeable barrier between the wound bed and external environment. The optimized hydrogel was found to significantly hasten skin regeneration in animal models when compared to other treatments in addition to strong inhibitory effect on the release of pro-inflammatory cytokines (interleukin-1β and tumor necrosis factor-α). Conclusions Our results suggest that sodium alginate -based hydrogels loaded with Chol-37(F34-R) hold the potential to be used as an alternative to conventional antibiotics in treating infectious skin wounds.

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Section: Methodsmentioning

confidence: 99%

Sustained release of alginate hydrogel containing antimicrobial peptide Chol-37(F34-R) in vitro and its effect on wound healing in murine model of Pseudomonas aeruginosa infection

Shi,

Dong,

Chen

et al. 2023

J Vet Sci

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“…The antibacterial activity of Chol-37(F34-R) was detected using the Kirby-Bauer diffusion method [ 40 ]. Four bacterial strains were used as experimental strains: L. monocytogenes CICC21634 cultured with BHI, P. aeruginosa GIM1.551 cultured with TSB, S. aureus ATCC25923, and S. typhimurium SL1344 cultured with LB medium.…”

Section: Methodsmentioning

confidence: 99%

Enhancing the antibacterial activity of antimicrobial peptide PMAP-37(F34-R) by cholesterol modification

et al. 2020

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Background The problem of increasing resistance against conventional antibiotics has drawn people’s attention. Therefore, the development of novel antibacterial agents with effective and safe therapeutic effects is imminent. Antimicrobial peptides (AMPs) are considered a promising class of antibacterial agents due to their broad antibacterial spectrum. Results In this study, on the basis of our previously studied peptide PMAP-37(F34-R), a novel antimicrobial peptide Chol-37(F34-R) was developed by N-terminal cholesterol modification to increase hydrophobicity. We observed that the N-terminal cholesterol-modified Chol-37(F34-R) showed higher antimicrobial activity than PMAP-37(F34-R) in vitro. Chol-37(F34-R) also exhibited effective anti-biofilm activity and may kill bacteria by improving the permeability of their membranes. Chol-37(F34-R) exerted high stability in different pH, salt, serum, and boiling water environments. Chol-37(F34-R) also showed no hemolytic activity and substantially low toxicity. Furthermore, Chol-37(F34-R) exhibited good potency of bacteria eradication and promoted wound healing and abscess reduction in infected mice. Meanwhile, in S. aureus ATCC25923-infected peritonitis model, Chol-37(F34-R) exhibited an impressive therapeutic effect by reducing the decrease in systemic bacterial burden and alleviating organ damage. Conclusions Our findings suggested that the N-terminal cholesterol modification of PMAP-37(F34-R) could improve antibacterial activity. Chol-37(F34-R) displayed excellent bactericidal efficacy and impressive therapeutic effect in vivo. Thus, Chol-37(F34-R) may be a candidate for antimicrobial agents against microbial infection in the clinic.

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“…To investigate the thermal stability of PMAP‐37 and its analogs, agar well diffusion was performed as reported previously (Pramono et al, ). Briefly, PMAP‐37 and its analogs were boiled for 0, 10, 20, 30, 40, 50, 60, 90, and 120 min, and filter paper containing the peptide that was boiled for different times was then prepared.…”

Section: Methodsmentioning

confidence: 99%

“…The antibacterial activity of PMAP‐37 and its analogs were detected using the Kirby‐Bauer diffusion method (Pramono et al, ). The ten bacterial strains listed in Section 2.1 were used as experimental strains, with L. monocytogenes CICC21533 was cultured with BHI, and other strains were cultured with Luria‐Bertani medium.…”

Section: Methodsmentioning

confidence: 99%

Enhancing the antibacterial activity of PMAP‐37 by increasing its hydrophobicity

et al. 2019

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With increasing resistance against conventional antibiotics, there is an urgent need to discover novel substances to replace antibiotics. This need provides an opportunity for the development of antimicrobial peptides (AMPs). To develop new AMPs with effective and safe therapeutic effects, two PMAP‐37 analogs called PMAP‐37(R13‐I) and PMAP‐37(K20/27‐I) were designed to increase hydrophobicity. Antimicrobial susceptibility testing and animal infection models were used to assess their antibacterial activity. The results showed that the minimal inhibitory concentrations of PMAP‐37(R13‐I) were lower than those of PMAP‐37 for two gram‐negative strains. Compared with PMAP‐37, PMAP‐37(K20/27‐I) not only inhibited the growth of most bacterial strains, but also exhibited antibacterial activity against Shigella flexneri CICC21534. In addition, PMAP‐37(K20/27‐I) exhibited pH and thermal stability. PMAP‐37(R13‐I) had a therapeutic effect only in mice infected with Salmonella typhimurium SL1344. However, PMAP‐37(K20/27‐I) exhibited the therapeutic effects, whether in the clinical symptoms, the tissue lesions, or the tissue bacterial loads and the survival rates in mice infected with Staphylococcus aureus ATCC25923 or S. typhimurium SL1344. Therefore, PMAP‐37(K20/27‐I) can be used as a substitute for antibiotics against infection with bacterial strains.

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In Vitro Assay of Ethanolic Heat Reflux Extract ofNicotiana tabacumL. var Virginia Against Nosocomial Bacteria Pathogen

Cited by 10 publications

References 3 publications

Sustained release of alginate hydrogel containing antimicrobial peptide Chol-37(F34-R) in vitro and its effect on wound healing in murine model of Pseudomonas aeruginosa infection

Sustained release of alginate hydrogel containing antimicrobial peptide Chol-37(F34-R) in vitro and its effect on wound healing in murine model of Pseudomonas aeruginosa infection

Enhancing the antibacterial activity of antimicrobial peptide PMAP-37(F34-R) by cholesterol modification

Enhancing the antibacterial activity of PMAP‐37 by increasing its hydrophobicity

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