In vitro artificial skin engineering by decellularized placental scaffold for secondary skin problems of meningomyelocele

Long-term preservation effects on biological properties of acellular placental sponge patches

“…The excellent cell adhesion and distribution were observed under SEM. Some investigators [38][39][40] reported the biocompatibility of the placenta-derived scaffolds for human foreskin fibroblasts, human epidermal keratinocytes, human amniotic membrane-derived stem cells [3] and mesenchymal cells [40].…”

Progress in developing decellularized bioscaffolds for enhancing skin construction

“…39 After decellularization, the acellular HAM retains all major structural components of the matrix and is biocompatible. 44 The cultured HAM-LSE is firm enough, with multilayered epidermis formation and epidermal stratification. 40 It has been demonstrated that the acellular HAM can be utilized as a feeder layer for some stem cells with induction of neuronal differentiation.…”

“…105 The construction of living skin equivalents (LSEs) using acellular HAM can be achieved by activation with platelet rich plasma, 41 and seeding keratinocytes on the epithelial side and fibroblasts on the chorionic side 42,43 or seeding mesenchymal stem cells, 106 even donor's own placental stem cells. 44 The cultured HAM-LSE is firm enough, with multilayered epidermis formation and epidermal stratification. After grafting onto full-thickness skin wounds of nude mice, HAM-LSE exhibits good morphology and good mechanical properties.…”

Desenvolvimento de >i<scaffolds>/i< de quitosana/gelatina/xerogel de tanino-glutaraldeído para aplicação em engenharia de tecidos

“…AHMADIAN; KARBASI, 2022), uma vez que são estruturas tridimensionais de suporte que mimetizam as propriedades e funções de tecidos biológicos, permitindo o desenvolvimento das células (VARSHNEY; KUMAR; KAMBLE, 2023), através da adesão, proliferação e migração celular, levando a formação de novos tecidos (DHANIA et al, 2022). Podem ser classificados de acordo com sua origem em derivados naturais (polímeros naturais), derivados sintéticos (polímeros sintéticos) e acelulares/descelularizados (originados de tecidos humanos ou animais tratados para eliminar células e antígenos, mas preservando sua estrutura) (SOMUNCU et al, 2019).…”