In Vitro Apoptosis Triggering in the BT-474 Human Breast Cancer Cell Line by Lyophilised Camel's Milk

Hasson, Sidgi S.; Al-Busaidi, Juma Zaid; Al-Qarni, Zahra; Rajapakse, Situsekara M W W B; Al-Bahlani, Shadia; Idris, Mohamed A.; Sallam, Talal A.

doi:10.7314/apjcp.2015.16.15.6651

Cited by 16 publications

(17 citation statements)

References 40 publications

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“…In agreement, previous studies also showed that camel milk induced apoptosis of MCF7 cells 6 and other cell lines, including HepG2, Hela, and BT-474, mainly through a similar molecular pathway. 6 , 10 , 28 This proapoptotic stimulatory effect for camel milk may be attributed to high content of lactoferrin and α-lactalbumin 10 ; however, to date the actual mediator(s) for camel milk involved in apoptosis has not elucidated yet. Lactoferrin inhibits proliferation in colorectal cancer cells through activation of Fas-dependent apoptotic pathway 29 ; however, in breast cancer cells, bovine milk–derived lactoferrin growth inhibitory effect was modulated by cell cycle arrest at the G1 and S phases, rather than by apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Effect of Camel Milk and Its Exosomes on MCF7 Cells In Vitro and In Vivo

Badawy

El-Magd

AlSadrah³

2018

Integr Cancer Ther

117

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Background/Objectives: In the Middle East, people consume camel milk regularly as it is believed to improve immunity against diseases and decrease the risk for cancer. Recently, it was noted that most of the beneficial effects of milk come from their nanoparticles, especially exosomes. Herein, we evaluated the anticancer potential of camel milk and its exosomes on MCF7 breast cancer cells (in vitro and in vivo) and investigated the possible underlying molecular mechanism of action. Methods/Results: Administration of camel milk (orally) and its exosomes (orally and by local injection) decreased breast tumor progression as evident by (a) higher apoptosis (indicated by higher DNA fragmentation, caspase-3 activity, Bax gene expression, and lower Bcl2 gene expression), (b) remarkable inhibition of oxidative stress (decrease in MDA levels and iNOS gene expression); (c) induction of antioxidant status (increased activities of SOD, CAT, and GPX), (d) notable reduction in expression of inflammation-(IL1b, NFκB), angiogenesis-(VEGF) and metastasis-(MMP9, ICAM1) related genes; and (e) higher immune response (high number of CD+4, CD+8, NK1.1 T cells in spleen). Conclusions: Overall, administration of camel milk–derived exosomes showed better anticancer effect, but less immune response, than treatment by camel milk. Moreover, local injection of exosomes led to better improvement than oral administration. These findings suggest that camel milk and its exosomes have anticancer effect possibly through induction of apoptosis and inhibition of oxidative stress, inflammation, angiogenesis and metastasis in the tumor microenvironment. Thus, camel milk and its exosomes could be used as an anticancer agent for cancer treatment.

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Section: Discussionmentioning

confidence: 99%

Therapeutic Effect of Camel Milk and Its Exosomes on MCF7 Cells In Vitro and In Vivo

Badawy

El-Magd

AlSadrah³

2018

Integr Cancer Ther

117

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“…Camel milk has been reported to possess anti-cancer activity as proved by a few of the in vitro and in vivo studies (Korashy et al, 2012a;Korashy et al, 2012b;Habib et al, 2013;Alhaider et al, 2014;Hasson et al, 2015). Lyophilized camel milk has been shown to inhibit growth and proliferation of many cancer cell types such as human breast cancer BT-474, laryngeal HE-p2 (Hasson et al, 2015), human hepatoma HepG2 (Korashy et al, 2012b) and murine hepatoma Hepa 1c1c7 cells (Korashy et al, 2012a). In addition camel milk protein 'lactoferrin' has been shown to inhibit the proliferation of human colorectal cancer HCT 116 cells by exerting antioxidant and DNA damage activities (Habib et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…It is being traditionally believed that drinking camel milk would help to fight against various human ailments including cancers, however, this proclaimed health benefits of the camel milk is not well recognized due to limited scientific study reports. A few of the in vitro studies have reported the inhibitory effects of camel milk on cancer cells exerted through the mechanism of apoptosis (Korashy et al, 2012b;Hasson et al, 2015) and antioxidant activities (Habib et al, 2013). Some of the in vivo studies have also shown the potent inhibitory effects of camel milk on pro-inflammatory, pro-angiogenic and pro-fibrogenic cytokines (Alhaider et al, 2014) as well as the ability of camel milk to modulate the expression of known cancer-activating gene and cancer-protective genes at the transcriptional and posttranscriptional levels (Korashy et al, 2012a).…”

Section: Introductionmentioning

confidence: 99%

Anticancer Activity of Camel Milk via Induction of Autophagic Death in Human Colorectal and Breast Cancer Cells

Krishnankutty

Iskandarani

Therachiyil

et al. 2018

Asian Pac J Cancer Prev

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Background/Objective: Camel milk is traditionally known for its human health benefits and believed to be a remedy for various human ailments including cancer. The study was aimed to evaluate the inhibitory effects of commercially available camel milk on cancer cells and its underlying mechanism(s). Materials and Methods: Two cell lines: colorectal cancer HCT 116 and breast cancer MCF-7 were cultured with different doses of camel milk. The effects of camel milk on cell death were determined by MTT assay, viability by trypan blue exclusion assay and migration by in vitro scratch assay. The mechanism was elucidated by western blotting and confocal microscopy was used to confirm autophagy. Results: Camel milk significantly reduced proliferation, viability as well as migration of both the cells. The accumulation of LC3-II protein along with reduction in expression of p62 and Atg 5-12, the autophagy proteins implied induction of autophagy. The (GFP)-LC3 puncta detected by confocal microscopy confirmed the autophagosome formation in response to camel milk treatment. Conclusion: Camel milk exerted antiproliferative effects on human colorectal HCT 116 and breast MCF-7 cancer cells by inducing autophagy.

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“…Lyophilized camel milk, primarily through the instigation of either the intrinsic and extrinsic apoptotic pathways, suppressed BT-474 human breast cancer cell growth and proliferation. The lyophilized camel milk also induced the caspase-3 mRNA, activity level and the initiation of DR4 in BT-474 cells [ 113 ]. Furthermore, the regulation of oxidative stress genetic signatures, heme-oxygenase-1 and reactive oxygen species production in BT-474 cells in response to lyophilized camel milk was reported by Hasson and his co-workers [ 113 ].…”

Section: Camel Milk As a Therapeutic Target In Cancer Treatmentmentioning

confidence: 99%

Research Development on Anti-Microbial and Antioxidant Properties of Camel Milk and Its Role as an Anti-Cancer and Anti-Hepatitis Agent

Khan

Xiao

et al. 2021

Antioxidants

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Camel milk is a rich source of vitamin C, lactic acid bacteria (LAB), beta-caseins and milk whey proteins, including lactoferrin, lysozyme, lactoperoxidase, alpha-lactalbumin and immunoglobulin. The lactoferrin plays a key role in several physiological functions, such as conferring antioxidant, anti-microbial and anti-inflammatory functions in cells. Similarly, the camel milk alpha-lactalbumin has shown greater antioxidative activity because of its higher antioxidant amino acid residues. The antioxidant properties of camel milk have also been ascribed to the structural conformation of its beta-caseins. Upon hydrolysis, the beta-caseins lead to some bioactive peptides having antioxidant activities. Consequently, the vitamin C in camel milk has a significant antioxidant effect and can be used as a source of vitamin C when the climate is harsh. Furthermore, the lysozyme and immunoglobulins in camel milk have anti-microbial and immune regulatory properties. The LAB isolated from camel milk have a protective role against both Gram-positive and -negative bacteria. Moreover, the LAB can be used as a probiotic and may restore the oxidative status caused by various pathogenic bacterial infections. Various diseases such as cancer and hepatitis have been associated with oxidative stress. Camel milk could increase antiproliferative effects and regulate antioxidant genes during cancer and hepatitis, hence ameliorating oxidative stress. In the current review, we have illustrated the anti-microbial and antioxidant properties of camel milk in detail. In addition, the anti-cancer and anti-hepatitis properties of camel milk have also been discussed.

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In Vitro Apoptosis Triggering in the BT-474 Human Breast Cancer Cell Line by Lyophilised Camel's Milk

Cited by 16 publications

References 40 publications

Therapeutic Effect of Camel Milk and Its Exosomes on MCF7 Cells In Vitro and In Vivo

Therapeutic Effect of Camel Milk and Its Exosomes on MCF7 Cells In Vitro and In Vivo

Anticancer Activity of Camel Milk via Induction of Autophagic Death in Human Colorectal and Breast Cancer Cells

Research Development on Anti-Microbial and Antioxidant Properties of Camel Milk and Its Role as an Anti-Cancer and Anti-Hepatitis Agent

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