In vitro antitrypanosomal and antileishmanial activity of plants used in Benin in traditional medicine and bio-guided fractionation of the most active extract

Béro, Joanne; Hannaert, Véronique; Châtaigné, Gabrielle; Hérent, Marie‐France; Quetin-Leclercq, Joëlle

doi:10.1016/j.jep.2011.07.022

Cited by 83 publications

(63 citation statements)

References 16 publications

(21 reference statements)

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“…The diversity of triterpenes is highly associated with their broad range of pharmacological effects, and different studies have already shown that these compounds present multispecies action against Leishmania sp. (Gnoatto et al., 2008, Bero et al., 2011, Begum et al., 2014). Recently, it was demonstrated that ursolic acid (UA) displayed activity against promastigote and amastigote forms of L. (L.) amazonensis , L. (V.) braziliensis , L. (L.) chagasi , and L. (L.) major (Passero et al., 2011, Begum et al., 2014, Yamamoto et al., 2014, Yamamoto et al., 2015), suggesting that UA presents multispectral action.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic effect of ursolic acid in experimental visceral leishmaniasis

Jesus

Fragoso

Yamamoto

et al. 2017

International Journal for Parasitology: Drugs and Drug Resistan

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Leishmaniasis is an important neglected tropical disease, affecting more than 12 million people worldwide. The available treatments are not well tolerated and present diverse side effects in patients, justifying the search for new therapeutic compounds. In the present study, the therapeutic potential and toxicity of ursolic acid (UA), isolated from the leaves of Baccharis uncinella C. DC. (Asteraceae), were evaluated in experimental visceral leishmaniasis. To evaluate the therapeutic potential of UA, hamsters infected with L. (L.) infantum were treated daily during 15 days with 1.0 or 2.0 mg UA/kg body weight, or with 5.0 mg amphotericin B/kg body weight by intraperitoneal route. Fifteen days after the last dose, the parasitism of the spleen and liver was stimated and the main histopathological alterations were recorded. The proliferation of splenic mononuclear cells was evaluated and IFN-γ, IL-4, and IL-10 gene expressions were analyzed in spleen fragments. The toxicity of UA and amphotericin B were evaluated in healthy golden hamsters by histological analysis and biochemical parameters. Animals treated with UA had less parasites in the spleen and liver when compared with the infected control group, and they also showed preservation of white and red pulps, which correlate with a high rate of proliferation of splenic mononuclear cells, IFN-γ mRNA and iNOS production. Moreover, animals treated with UA did not present alterations in the levels of AST, ALT, creatinine and urea. Taken together, these findings indicate that UA is an interesting natural compound that should be considered for the development of prototype drugs against visceral leishmaniasis.

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Section: Introductionmentioning

confidence: 99%

Therapeutic effect of ursolic acid in experimental visceral leishmaniasis

Jesus

Fragoso

Yamamoto

et al. 2017

International Journal for Parasitology: Drugs and Drug Resistan

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“…The bisnortriterpenes from Salacia madagascariensis (Celastraceae); isoiguesterin (55) (57) Me H oleanolic acid (58) Keetia leucantha (synonym: Plectronia leucantha Krause) is a West African tree of the Rubiaceae, used to treat a variety of infections, including parasitic infections. 136,137 Ursolic acid (57) and oleanolic acid (58), along with other constituents were isolated from the leaves of this plant. An investigation of the antitrypanosomal activities of essential oil, the dichloromethane extract and isolated compounds on T. b. brucei bloodstream forms (Tbb BSF) and procyclic forms (Tbb PF) 138 showed that ursolic acid (57) and oleanolic acid (58) were the most bioactive tested compounds.…”

Section: Triterpenoidsmentioning

confidence: 99%

Compounds from African Medicinal Plants with Activities against Protozoal Diseases: Schistosomiasis, Trypanosomiasis and Leishmaniasis

Cv¹,

Ntie‐Kang²,

Sh.³

et al. 2018

Preprint

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An absolute configuration of 5R seems to decrease activity of the compound IntroductionProtozoa may be considered as microscopic, essentially single-celled, eukaryotic organisms that are free-living or parasitic (obtaining their food by eating other organisms or their products) in nature. Parasitic protozoal diseases continue to be a cause of considerable morbidity and mortality globally. 1,2Parasitic protozoal diseases include malaria, 3,4 trypanosomiasis (African sleeping sickness and Chagas disease), 5-7 leishmaniasis 8 and schistosomiasis. 9,10 They threaten almost one-third of the world's population, the most numerous incidents being recorded in over 100 Tropical and developing countries and territories, Figure 1. 11,12 Malaria, for example, was reported by the WHO, to be responsible for approximately 214 million sickness cases and 438,000 deaths globally in 2017. 13 The African region recorded the most death-related cases, especially amongst infants below the age of 5 and pregnant women. Schistosomiasis, caused by parasites of the Schistosoma genus are responsible for about 200 million sickness cases and about 280,000 death-related incidents annually worldwide.9,10,14 Only one drug (praziquantel) has been proven to be effective in the treatment of human schistosomiasis, with no vaccine available or in development so far.15-21 Serious concerns about drug selectivity and resistance were raised in 2013 when over 30 million people were treated in Sub-Saharan Africa. 20 Moreover, observed resistance and reduced efficiency of praziquantel in laboratory strains have prompted the search for alternative therapeutic strategies. Trypanosomiasis, which represents several diseases caused by parasites of the genus Trypanosoma, is also of interest. 5,27,29 This disease, which is much arguably the most important disease of man and domesticated animals, accounts for over 8 million reported annual cases globally, especially in the tropical regions of Latin America and Africa. 30,31 Although the present number of cases seems negligible on a worldwide scale, great socioeconomic effects on the endemic areas by this disease are forecast if inadequate attention (both at the communal, national, and international levels) is not given. 7,29,[32][33][34] Leishmaniasis is caused by parasites of the Leishmania type, which is also transmitted by certain types of sandflies. 35,36 The diseases are reported by the WHO to be responsible for about 1 million new cases leading to approximately 30,000 deaths annually on a global scale. The major cause is linked to environmental changes A R T I C L E I N F O A B S T R A C TArticle history:English abstract: Parasitic diseases continue represent a threat on a global scale, particularly among the poorest countries in the world. This is particularly because of the absence of vaccines, and in some cases, resistance against available drugs, currently being used for their treatment. In this review emphasis is laid on natural products and scaffolds from African medicinal plants (AMPs) for lead drug di...

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“…In these days ten million people suffer from cutaneous leishmaniasis. One in twenty individuals worldwide is suffered from cutaneous leishmaniasis [7]. …”

Section: Introductionmentioning

confidence: 99%

In VitroAntimicrobial and Antiprotozoal Activities, Phytochemical Screening and Heavy Metals Toxicity of Different Parts ofBallota nigra

Ullah

Ahmad

Ayaz

2014

BioMed Research International

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The study was done to assess the phytochemicals (flavonoids, terpenoids, saponins, tannin, alkaloids, and phenol) in different parts (root, stem, and leaves) of Ballota nigra and correlated it to inhibition of microbes (bacteria and fungi), protozoan (Leishmania), and heavy metals toxicity evaluation. In root and stem flavonoids, terpenes and phenols were present in ethanol, chloroform, and ethyl acetate soluble fraction; these were found to be the most active inhibiting fractions against all the tested strains of bacteria, fungi, and leishmania. While in leaves flavonoids, terpenes, and phenols were present in ethanol, chloroform, and n-butanol fractions which were the most active fractions against both types of microbes and protozoan (leishmania) in in vitro study. Ethanol and chloroform fractions show maximum inhibition against Escherichia coli (17 mm). The phytochemical and biological screenings were correlated with the presence of heavy metals in selected plant Ballota nigra. Cr was found above permissible value (above 1.5 mg/kg) in all parts of the plant. Ni was above WHO limit in B. nigra root and leaves (3.35 ± 1.20 mg/kg and 5.09 ± 0.47 mg/kg, respectively). Fe was above permissible value in all parts of B. nigra (above 20 mg/kg). Cd was above permissible value in all parts of the plant (above 0.3 mg/kg). Pb was above WHO limit (above 2 mg/kg) in all parts of Ballota nigra.

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In vitro antitrypanosomal and antileishmanial activity of plants used in Benin in traditional medicine and bio-guided fractionation of the most active extract

Cited by 83 publications

References 16 publications

Therapeutic effect of ursolic acid in experimental visceral leishmaniasis

Therapeutic effect of ursolic acid in experimental visceral leishmaniasis

Compounds from African Medicinal Plants with Activities against Protozoal Diseases: Schistosomiasis, Trypanosomiasis and Leishmaniasis

In VitroAntimicrobial and Antiprotozoal Activities, Phytochemical Screening and Heavy Metals Toxicity of Different Parts ofBallota nigra

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